FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orthomyxovirus.NODE_2459.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orthomyxovirus.NODE_2459.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCTTCTGGCACAACGACACTGGCTACTG1434.359756097560975No Hit
TCACGAACACCTCTGTAGATAGCAGCGAG1123.414634146341464No Hit
TAGCAGCGAGACACGAACCGGCGACATGG962.9268292682926833No Hit
TCCGATTGACGGCTTCGCTTCTGGCACAAC902.7439024390243905No Hit
TAGCAGCGAGACACGAACCGGCGACATGGC812.4695121951219514No Hit
TAAACTGGCAGCATCGGTCGTACTTTGATC461.402439024390244No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAAC401.2195121951219512No Hit
TGTTTCCCTCCCCACCCACTCTTTTCCACT391.1890243902439024No Hit
TAAGTATGATGTATACCTTGGGTGGAGCC351.0670731707317074No Hit
TTCACGAACACCTCTGTAGATAGCAGCGA320.975609756097561No Hit
TAAGGGCTTCCACGAACCTCAGCTTTGCCT320.975609756097561No Hit
TTCACGAACACCTCTGTAGATAGCAGCGAG290.8841463414634145No Hit
TCAAAACCTCCCTCTATTGTAGATTTGAGC280.853658536585366No Hit
TCATTTCTCGAGAGCGCTTGTTTGGGGG270.8231707317073171No Hit
TGATGAACTTTTTGATCTAAACTGGCAGC260.7926829268292683No Hit
TTCCGGACACCATGAACTTCATATCCACCT220.6707317073170732No Hit
TAGATAGCAGCGAGACACGAACCGGCGAC210.6402439024390244No Hit
TGATAAAGGACGGTAGACCGTGATGGGA210.6402439024390244No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAACA200.6097560975609756No Hit
TCTGGGAGAGGACTCTCCGTTCGACCGCC190.5792682926829268No Hit
TAAACATGCACCCCGGTACTTTTCCTTGC190.5792682926829268No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAA190.5792682926829268No Hit
TACTTCGATCAACTCTGACAGGATCAGTC190.5792682926829268No Hit
TCTGGGAGAGGACTCTCCGTTCGACCGCCT180.548780487804878No Hit
TCAAACGGTTTAGTTGCAGTAGGGGTTT170.5182926829268293No Hit
TCTTACTTCGATCAACTCTGACAGGATCAG160.4878048780487805No Hit
TGTCCAAGTCTTAATTCCTTGAGTCGGAGCC150.45731707317073167No Hit
TCCGGACACCATGAACTTCATATCCACCTC150.45731707317073167No Hit
TTGTAAGTGCGCACTAGCTCCTCTGAAGTT150.45731707317073167No Hit
TCCCTTCCACTGCCAGTGAGTTAACCAGTT150.45731707317073167No Hit
TAAGTGCGCACTAGCTCCTCTGAAGTTGG140.426829268292683No Hit
GACACAAACCGGCGACATGGCACTCATAAGC140.426829268292683No Hit
TCCTAGGCGACACCGTTTGGCAACGGGC140.426829268292683No Hit
TGTTGCAGAAAGAAACTCTGTCGCCGAACCT140.426829268292683No Hit
TCTGTCGTTTCGTATAAACATGCACCCCGG140.426829268292683No Hit
TAGATAGCAGCGAGACACGAACCGGCG130.39634146341463417No Hit
TCCACGAACCTCAGCTTTGCCTCTTCGGCAGT130.39634146341463417No Hit
TGATAAAGGACGGTAGACCGTGATGGGAA120.3658536585365854No Hit
TAGCAGCGAGACACAAACCGGCGACATGG120.3658536585365854No Hit
TTCAAAATCCCTCGCACCCTGTCCTTGTC120.3658536585365854No Hit
TGGGCCCAAATTTCTGGAGGATGTTGATC120.3658536585365854No Hit
TTAGACTTGTTTCCCTCCCCACCCACTCT120.3658536585365854No Hit
TTTGACTTCAATCAATGACTGCCTCCCCT110.3353658536585366No Hit
TCCGATTGACGGCTTCGCTTCTGGCACA110.3353658536585366No Hit
TACTTCGATCAACTCTGACAGGATCAGTCT110.3353658536585366No Hit
TCTGGCCTGTGGTTGTGGTTGTTGTTGT110.3353658536585366No Hit
TACGTGATGACGAGCTTCCAGGCCCAGAGT110.3353658536585366No Hit
TCCGGACACCATGAACTTCATATCCACCTCC110.3353658536585366No Hit
TATCCTAGGCGACACCGTTTGGCAACGGGC110.3353658536585366No Hit
TCAAAATCCCTCGCACCCTGTCCTTGTCTT110.3353658536585366No Hit
TCCAGTCCCTTCCACTGCCAGTGAGTTAAC110.3353658536585366No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAACAA100.3048780487804878No Hit
TCCCCTAGGTCTAAGTGTTGATAGAATCT100.3048780487804878No Hit
TTTCCGGACACCATGAACTTCATATCCACCT100.3048780487804878No Hit
CCTCCGATTGACGGCTTCGCTTCTGGCACA100.3048780487804878No Hit
TGCACCCCGGTACTTTTCTTTGCACTGAAAC100.3048780487804878No Hit
TGTGAGCTCGAGCTTTGTCAATCTGACAGC100.3048780487804878No Hit
GACACGAACCGGCGACATGGCACTCATATGC100.3048780487804878No Hit
TCCGATTGACGGCTTCGCTTCTGGCACAA100.3048780487804878No Hit
TTCAAACGGTTTAGTTGCAGTAGGGGTT90.274390243902439No Hit
TCATTTCTCGAGAGCGCTTGTTTGGGGGC90.274390243902439No Hit
TGAAGACGGTGCCCCGGAAGACAGTGCGT90.274390243902439No Hit
TCAGTCTTACTTCGATCAACTCTAACAGGT90.274390243902439No Hit
TCTGTAGATAGCAGCGAGACACGAACCGGC90.274390243902439No Hit
TGTTTCAGAAAGAAACTCTGTCGCCGAACCT90.274390243902439No Hit
CTCCGATTGACGGCTTCGCTTCTGGCACA90.274390243902439No Hit
TATACCTTGGGTGGAGCCGGAGCGCACTG90.274390243902439No Hit
TCACGAACACCTCTGTAGATAGCAGCGA90.274390243902439No Hit
TGATGAACTTCTTGATCTAAACTGGCAGC90.274390243902439No Hit
TGAAGACGGTGCCCCGGAATACAGTGCGT80.24390243902439024No Hit
TCTTACTTCGATCAACTCTGACGGGATCAG80.24390243902439024No Hit
AACCTGGGTGGAACAAGTAAACTGAAGTCT80.24390243902439024No Hit
TGGGCCCAAATTTCTGGAGGATGTCGATCA80.24390243902439024No Hit
CCTCCGATTGACGGCTTCGCTTCTGGCAC80.24390243902439024No Hit
TTTGGCAACGGGCGCCTAGTTTAAGGGCTT80.24390243902439024No Hit
TTCAAAATCCCTCGCACCCTGTCCTTGTCT80.24390243902439024No Hit
TTTTACTTCGATCAACTCTGACAGGATCAG80.24390243902439024No Hit
CTCCGATTGACGGCTTCGCTTCTGGCACAAC80.24390243902439024No Hit
TAAACATGCACCCCGGTACTTTTCTTTGCAC70.2134146341463415No Hit
CACGACACACTCCCCATGATTTATGGCTG70.2134146341463415No Hit
TTGGGAACGGGCGCCTAGTTTGAGGGCTT70.2134146341463415No Hit
TCCCTCGCACCCTGTCCTTGTCTTTATGTT70.2134146341463415No Hit
TCCACGAACCTCAGCTTTGCCTCTTCGG70.2134146341463415No Hit
TACGAACCTCAGCTTTGCCTCTTCGGCAGT70.2134146341463415No Hit
TACGTGATGACGAGCTTCCAGGCCCAGAG70.2134146341463415No Hit
TCCAAAACCACGACACACTCCCCATGATTT70.2134146341463415No Hit
TAGCAGCGAGACACGAACCGGCGACATGGCAC70.2134146341463415No Hit
TGTAGATAGCAGCGAGACACGAACCGGCG70.2134146341463415No Hit
TCCCCATGATTTATGGCTGACACTGTCCCATC70.2134146341463415No Hit
CACGAACACCTCTGTAGATAGCAGCGAG70.2134146341463415No Hit
ATAGCAGCGAGACACAAACCGGCGACATGG60.1829268292682927No Hit
TCAATCAATGACTGCCTCCCCTTCTTAAGA60.1829268292682927No Hit
TAAGGGCTTCCACGAACCTCAGCTTTGCC60.1829268292682927No Hit
TGTTGCAGAAAGAAACTCTGTCGCCGAACC60.1829268292682927No Hit
TCCACGAACCTCAGCTTTGCCTCTTCGGC60.1829268292682927No Hit
TTCAGCCTGGTTGCCTGGCCACCCTCAGAA60.1829268292682927No Hit
TAGCACCCTGTCCTTGTCTTTATGTTGGC60.1829268292682927No Hit
TCTTACTTCGATCAACTCTGACAGGATCAGTC60.1829268292682927No Hit
TAAGAAGAACTTTCCGGACACCATGAACT60.1829268292682927No Hit
TAGTCTGATATGTTCGATTAGTGTCGAGT60.1829268292682927No Hit
TACCTTGGGTGGAGCCGGAGCGCACTGG60.1829268292682927No Hit
TAAGACATTCCAGTCCCTTCCACTGCCAGT60.1829268292682927No Hit
TATTCATTTCTCGAGAGCGCTTGTTTGGG60.1829268292682927No Hit
TGTTTCACGAACACCTCTGTAGATAGCAGC60.1829268292682927No Hit
TCGCTTCTGGCACAACGACACTGGCTACTG60.1829268292682927No Hit
CACGAACCTCAGCTTTGCCTCTTCGGCAGT60.1829268292682927No Hit
TTCCAGTCCCTTCCACTGCCAGTGAGTTAAC60.1829268292682927No Hit
TCAAAATCCCTCGCACCCTGTCCTTGTCT60.1829268292682927No Hit
TGATGAACTTCTTGATCTAAACTGGCAGGA60.1829268292682927No Hit
TCTTACTTCGATCAACTCTGACGGGATCAGT60.1829268292682927No Hit
TTGACGGCTTCGCTTCTGGCACAACGACAC50.1524390243902439No Hit
TGACGGCTTCGCTTCTGGCACAACGACAC50.1524390243902439No Hit
TACTTCTTCGGCAGTGTCTGGCCTGTGGT50.1524390243902439No Hit
TAGCAGCGAGACACAAACCGGCGACATGGC50.1524390243902439No Hit
TCAGAAAGAAACTCTGTCGCCGAACCTCC50.1524390243902439No Hit
TCAACTCTGACGGGATCAGTCTTACTTCGATCAAC50.1524390243902439No Hit
TCCAAATTCGCTTCTTGTAGCCCACTCTT50.1524390243902439No Hit
CGCACTGGGCCCAAATTTCTGGAGGATGTC50.1524390243902439No Hit
TACGTGATGACGAGCTTCCAGTCCCAGAG50.1524390243902439No Hit
TCAGTCTTACTTCGATCAACTCTGACGGG50.1524390243902439No Hit
TCTTACTTCGATCAACTCTGACAGGATCAGT50.1524390243902439No Hit
TTCAAACGGTTTAGTTGCAGTAGGGGTTT50.1524390243902439No Hit
TACTGGCAGCATCGGTCTTACTTCGATCAAC50.1524390243902439No Hit
TTCCACGAACCTCAGCTTTGCCTCTTCCT50.1524390243902439No Hit
TTCCCCTAGGTCTAAGTGTTGATAGAATC50.1524390243902439No Hit
TCACATTCAGCCTGGTTGCCTGGCCACCCT50.1524390243902439No Hit
TTCGCTTCTTGTAGCCCACTCTTTTTCGGA50.1524390243902439No Hit
TCCAAAACCACGACACACTCCCCATGATT50.1524390243902439No Hit
TCATATCCACCTCCTCTCCCAACTTAGACT50.1524390243902439No Hit
TCTGTCGCCGAACCTCCGATTGACGGCTTC50.1524390243902439No Hit
CCTCCGATTGACGGCTTCGCTTCTGGCACAA50.1524390243902439No Hit
TAAACATGCACCCCGGTACTTTTCTTTGCA50.1524390243902439No Hit
TCATAGCCTCTCCAACCTTCACCAGGAAA50.1524390243902439No Hit
TTTCCGGACACCATGAACTTCATATCCACC50.1524390243902439No Hit
TCATAGCCTCTCCAACCTTCACCAGGAAAG50.1524390243902439No Hit
TCGGCAGTGTCTGGCCTGTGGTTGTGGTT50.1524390243902439No Hit
TGTTGATAAAGGACGGTAGACCGTGATGG50.1524390243902439No Hit
TTTCTCGAGAGCGCTTGTTTGGGGGCATC50.1524390243902439No Hit
TAGCAGCGAGACACGAACCGGCGACATG50.1524390243902439No Hit
TAAGTGCGCACTAGCTCCTCTGAAGTTGGT50.1524390243902439No Hit
TACTTCGATCAACTCTGACGGGATCAGTCT50.1524390243902439No Hit
TGCAGTCATTCACGACGTGCCAGTGGTCGT40.12195121951219512No Hit
TGAGCTCGAGCTTTGTCAATCTGACAGGAT40.12195121951219512No Hit
TTTTGACTTCAATCAATGACTGCCTCCCC40.12195121951219512No Hit
TTTGACACAAGCTGTTAGCGGGCCCAGACT40.12195121951219512No Hit
TTCAGAAAGAAACTCTGTCGCCGAACCTCC40.12195121951219512No Hit
TTCGGCAGTGTCTGGCCTGTGGTTGTGGT40.12195121951219512No Hit
TCCGATTGACGGCTTCGCTTCTGGCAC40.12195121951219512No Hit
TGCACAAGCTGTGGGTTCTTACTTATGAGT40.12195121951219512No Hit
TTGGGCCATGGACGACTGTACATCCGGT40.12195121951219512No Hit
AAGGGCTTCCACGAACCTCAGCTTTGCCT40.12195121951219512No Hit
TGAAACATTATCTGGGAGAGGACTCTCCGTT40.12195121951219512No Hit
CACCCCGGTACTTTTCCTTGCACTGAAACAA40.12195121951219512No Hit
TCTGTCGTTTCGTATAAACATGCACCCCGGT40.12195121951219512No Hit
TTCCGGACACCATGAACTTCATATCCACC40.12195121951219512No Hit
TGTAAGTGCGCACTAGCTCCTCTGAAGTT40.12195121951219512No Hit
TAGATAGCAGCGAGACACAAACCGGCG40.12195121951219512No Hit
TTCCACGAACCTCAGCTTTGCCTCTTCGG40.12195121951219512No Hit
TTCGACAACACCGTTCATATCCCTCTTGT40.12195121951219512No Hit
TTTTACTTCGATCAACTCTGACAGGATCAGTC40.12195121951219512No Hit
TTCGCTTCTGGCACAACGACACTGGCTACT40.12195121951219512No Hit
TCCAAGTCTTAATTCCTTGAGTCGGAGCCC40.12195121951219512No Hit
TAGCACCCTGTCCTTGTCTTTATGTTGG40.12195121951219512No Hit
TCCAAGTCTTAATTCCTTGAGTCGGAGCC40.12195121951219512No Hit
TCCAGTCCCAGAGTGCTCACATTCAGCCTG40.12195121951219512No Hit
ACCTGGGTGGAACAAGTAAACTGAAGTCT40.12195121951219512No Hit
TTCTGCCCGCTACTAACGTTTGATATGTTT40.12195121951219512No Hit
TCCCATCGTCATAGCCTCTCCAACCTTC40.12195121951219512No Hit
TACCTTGGGTGGAGCCGGAGCGCACTG40.12195121951219512No Hit
TCCCTCGCACCCTGTCCTTGTCTTTATGT40.12195121951219512No Hit
TCGTATAAACATGCACCCCGGTACTTTTC40.12195121951219512No Hit
TGTCGATCAAAACCTCCCTCTATTGTAGA40.12195121951219512No Hit
CACGAACCTCAGCTTTGCCTCTTCGGCAGTA40.12195121951219512No Hit
TGAACTTCATATCCACCTCCTCTCCCAACT40.12195121951219512No Hit
TCAAAACCTCCCTCTATTGTAGATTTGAGCATAGA40.12195121951219512No Hit
TGCACCCCGGTACTTTTCTTTGCACTGAAACA40.12195121951219512No Hit
TCGGCAGTGTCTGGCCTGTGGTTGTGGT40.12195121951219512No Hit
TACTTCGATCAACTCTGACAGGATCAGTT40.12195121951219512No Hit
CACGAACACCTCTGTAGATAGCAGCGAGA40.12195121951219512No Hit
TTGGGAACGGGCGCCTAGTTTGAGGGCTTC40.12195121951219512No Hit
TAGATAGGTCCTGGGCTGATGTGAGCT40.12195121951219512No Hit
TAAGTGAACGAGATAAGAGGGCTCATAAC40.12195121951219512No Hit
TCCACGAACCTCAGCTTTGCCTCTTCCGGC40.12195121951219512No Hit
TTGATAAAGGACGGTAGACCGTGATGGG40.12195121951219512No Hit
TCTTGTAAGACATTCCAGTCCCTTCCACTGC40.12195121951219512No Hit
TACGAACCTCAGCTTTGCCTCTTCGGCAGTA40.12195121951219512No Hit
TACGAACCTCAGCTTTGCCTCTTCGGCAG40.12195121951219512No Hit
TCTGGCACAACGACACTGGCTACTGGTGT40.12195121951219512No Hit
TCAATGGCAAGTCCGTTCTTGTGGGCATC40.12195121951219512No Hit
TTCAGGGTGCTGAGATGAATTGAAATGGA40.12195121951219512No Hit
TCTCTCGCACCCTGTCCTTGTCTTTATGT40.12195121951219512No Hit
TCTGGCCTGTGGTTGTGGTTGTTGTTGTT40.12195121951219512No Hit
TAAGAAGAACTTTCCGGACACCATGAACTT40.12195121951219512No Hit
TTGGCAACGGGCGCCTAGTTTAAGGGCTT40.12195121951219512No Hit
TGAACTTCTTGATCTAAACTGGCAGGATC40.12195121951219512No Hit
TCTGTAGATAGCAGCGAGACACAAACCGGC40.12195121951219512No Hit
TACGTGTCCTTTCTGCCCGCTACTAACGT40.12195121951219512No Hit
TCCACGAACCTCAGCTTTGCCTCTTCG40.12195121951219512No Hit
TACGTGATGACGAGCTTCCAGTCCCAGAGT40.12195121951219512No Hit
TGCCTCTTCGGCAGTGTCTGGCCTGTGGT40.12195121951219512No Hit
AGCGAGACACAAACCGGCGACATGGCACTC40.12195121951219512No Hit
TCTCTCGCACCCTGTCCTTGTCTTTATGTT40.12195121951219512No Hit
CGTATAAACATGCACCCCGGTACTTTTCC40.12195121951219512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)