FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orthomyxovirus.NODE_284.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orthomyxovirus.NODE_284.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5608
Sequences flagged as poor quality0
Sequence length16-35
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACGAGGAACACCTTACTCAGGAGGA321857.38231098430813No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAGC1903.3880171184022827No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAA1121.9971469329529243No Hit
GAGGAACGAGGAACACCTTACTCAGGAGG661.1768901569186876No Hit
AACCACTGGCTGCTTTCTCAAATCGTCGAG611.087731811697575No Hit
TGGCACTCTTGTTGAGATTCTCGGATGAG571.0164051355206847No Hit
TCAAATCGTCGAGGACGCACTAGTAAGC560.9985734664764622No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAGG480.8559201141226819No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGC430.7667617689015691No Hit
TCGACATAGCCCTTAACATTTACAACCACT420.7489300998573466No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAG410.7310984308131241No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAGC390.6954350927246791No Hit
TCAAATCGTCGAGGACGCAGTAGTAAGC340.6062767475035664No Hit
AGGAACGAGGAACACCTTACTCAGGAGGA310.5527817403708988No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAG290.5171184022824536No Hit
TCGTCGAGGTCGCACTAGTAAGCAATACT250.4457917261055635No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAGT220.39229671897289586No Hit
TTCTCAAATCGCCGAGGACGCACTAGTAAGC200.3566333808844508No Hit
TGGCACTCTTGTGGAGATTCTCGGATGAG200.3566333808844508No Hit
CCACTGGCTGCTTTCTCAAATCGTCGAGG200.3566333808844508No Hit
TAGCTGCTTTCTCAAATCGTCGAGGACGC200.3566333808844508No Hit
TCCCAAATCGTCGAGGACGCACTAGAAAGC190.3388017118402282No Hit
TCCGACTCTCGTCTTATTCTGTACCTTCCT190.3388017118402282No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTC180.3209700427960057No Hit
TGTAATCATCTTCAAGTGCTGGGCGCTCC160.28530670470756064No Hit
TAACATTTACAACCACTGGCTGCTTTCTC160.28530670470756064No Hit
TTGTAATCATCTTCAAGTGCTGGGCGCTCC150.2674750356633381No Hit
TCAAATCGTCGAGGTCGTGATAGAAACCA140.24964336661911554No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAGC130.23181169757489303No Hit
TCAAATCGTCGAGGACGCACTAGTAAG120.21398002853067047No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAG120.21398002853067047No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAAAG110.19614835948644793No Hit
TGCTTTCTCAAATCGTCGAGGACGCACT110.19614835948644793No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAG110.19614835948644793No Hit
TTGTAATCATCTTCAAGTGCTGGGCGCTC100.1783166904422254No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGCA100.1783166904422254No Hit
TACATGCAGCCCATTTGGCCTTCCCGCCAGC100.1783166904422254No Hit
TTGGCTCTGTTGGCTGGCGTCGAGATGGT100.1783166904422254No Hit
TGCATGCAGCCCATTTGGCCTTCCCGCCAGC90.16048502139800286No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAAA90.16048502139800286No Hit
TCGGGAATTCTCCCTCGCCAAAGCCGGAT90.16048502139800286No Hit
TTAACATTTACAACCACTGGCTGCTTTCTC90.16048502139800286No Hit
CTGGCTGCTTTCTCAAATCGTCGAGGACGC80.14265335235378032No Hit
TGCTTTCTCAAATCGTCGAGGTCGTGAT80.14265335235378032No Hit
GAGGAACGAGGAACACCTTACTCAGGAG80.14265335235378032No Hit
TCTGTACGGAAAGGTTGGCGGGTTGCCGGC80.14265335235378032No Hit
TCGTCGAGGACGCAGTAGTAAGCAATACTT80.14265335235378032No Hit
TCCCGATCCACCAATCTAGAGCCTCTCTCT80.14265335235378032No Hit
TTATCAGCGTGGTGTGTGGCGGTGCCTGC80.14265335235378032No Hit
TGTAATGTGAGAGAATGAGCCTGGAGCGCC80.14265335235378032No Hit
TATGTTTGCACCCAACCCGATTGATGAC80.14265335235378032No Hit
TTCTCAAATCGTCGAGGTCGTGATAGAAAC70.12482168330955777No Hit
TGTGATAGAAGACTCGAAGGTACCACTTCC70.12482168330955777No Hit
TGATACTCGAAGAACACTTTCTCCCTCCCT70.12482168330955777No Hit
CTCAAATCGCCGAGGACGCACTAGTAAGC70.12482168330955777No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAGGT70.12482168330955777No Hit
TACCACTTCCGACTCTCGTCTTATTCTGT70.12482168330955777No Hit
TCTCAAATCCTCGAGGACGCACTAGTAAGC70.12482168330955777No Hit
TCTCAAATCGTCGAGGTCGTGATAGAAACC70.12482168330955777No Hit
TAACTTTCCGCCCCGCCCTTAGCAAAAG70.12482168330955777No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAG70.12482168330955777No Hit
TTTGTCAGCGTGGTGTGTGGCGGTGCCT70.12482168330955777No Hit
TGAGGAGACTCTTGATGACAGATTTGCCT70.12482168330955777No Hit
TGAGGAACGAGGAACACCTTACTCAGGAGGA70.12482168330955777No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAG70.12482168330955777No Hit
TCCCAAATCGTCGAGGTCGTGATAGAAACC70.12482168330955777No Hit
TCTCAAATCGTCGAGGTCGTGATAGAAAC70.12482168330955777No Hit
TAACATTTACAACTGGCTGCTTTCCCAAA60.10699001426533523No Hit
TTTGGCCTTCCCGCCAGCCCATCCACCTT60.10699001426533523No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGGA60.10699001426533523No Hit
TCCCCTTTTGGAAGTTTGAACATGCCCTAGA60.10699001426533523No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGGT60.10699001426533523No Hit
TCAAATCGCCGAGGACGCACTAGTAAGC60.10699001426533523No Hit
TTGGCTGGCGTCGAGATGGTGATGAGGA60.10699001426533523No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAG60.10699001426533523No Hit
TGGCTGCTTTCTCAAATCGTCGAGGTCGT60.10699001426533523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)