FastQCFastQC Report
Wed 31 Mar 2021
S33.15-35nt.orthomyxovirus.NODE_62727.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.orthomyxovirus.NODE_62727.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length26-33
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTCACCAATGACGCCCGATGCGACAGC1113.095238095238097No Hit
TAAATCTCTGACATCACTTGGTAAGCGGT67.142857142857142No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAG44.761904761904762No Hit
TGAACCTCCTTGTAGCTCTTCTTTAGCACC44.761904761904762No Hit
TTTTGAAAGGACCTCTGGAATCTTTGACT33.571428571428571No Hit
TCATAAATCTCTGACATCACTTGGTAAGC33.571428571428571No Hit
TATTGTGGAGCCAGGCAGATATTTCCCA33.571428571428571No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTA33.571428571428571No Hit
TACTTGGGCACAGTGTTCACCAATGACGCC33.571428571428571No Hit
TTATTGTGGAGCCAGGCAGATATTTCCCA22.380952380952381No Hit
TCACTTGGTAAGCGGTTGCAGAAGGCACG22.380952380952381No Hit
TAGTAGTATGGGGTTTTTTTAAAGGT22.380952380952381No Hit
TCTGAACCTCCTTGTAGCTCTTCTTTAGCACC22.380952380952381No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGT22.380952380952381No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGG22.380952380952381No Hit
TCTAGCTTAGCCCTTTCTCCGTGTTTGATA22.380952380952381No Hit
TTGGCATATTAAAAGGGGCTTTTTGGAAAC22.380952380952381No Hit
TCTTTTTCTTTTCTTCTCCGCCTATGCTT22.380952380952381No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGGAT11.1904761904761905No Hit
TCACTTGGTAAGCGGTTGCAGAAGGCAC11.1904761904761905No Hit
TCTTTGACTCTCTTCTGAACCTCCTT11.1904761904761905No Hit
TTTTCATAAATCTCTGACATCACTTGGTA11.1904761904761905No Hit
TGCGATCTAGCTTAGCCCTTTCTCCGTGT11.1904761904761905No Hit
AGCTCTTCTTTAGCACCATTATTTCATC11.1904761904761905No Hit
TCACCAATGACGCCCGATGCGACAGCGAAAT11.1904761904761905No Hit
TCTTCCCCAAATTCTTTTCATAAATCTCTG11.1904761904761905No Hit
TAGCTCTTCTTTAGCACCATTATTTCATC11.1904761904761905No Hit
TGTTCACCAATGACGCCCGATGCGACAG11.1904761904761905No Hit
GTTCACCAATGACGCCCGATGCGACAG11.1904761904761905No Hit
CTAGCTTAGCCCTTTCTCCGTGTTTGATA11.1904761904761905No Hit
TCTAGCTTAGCCCTTTCTCCGTGTTTGAT11.1904761904761905No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGGA11.1904761904761905No Hit
TACACTGTAGATGATGATATTATTGGAGG11.1904761904761905No Hit
TCTTTGACTCTCTTCTGAACCTCCTTGT11.1904761904761905No Hit
TGCTTATTGTGGAGCCAGGCAGATATTTC11.1904761904761905No Hit
TCTGAACCTCCTTGTAGCTCTTCTTTAGCACCA11.1904761904761905No Hit
TCTGGAATCTTTGACTCTCTTCTGAACCT11.1904761904761905No Hit
TATTGTGGAGCCAGGCAGATATTTCCCAAA11.1904761904761905No Hit
TAGTTACAGTGACATTCTTTTTGAAAGGACCTC11.1904761904761905No Hit
TAGCTTAGCCCTTTCTCCGTGTTTGATA11.1904761904761905No Hit
AGTAGTATGGGGTTTTTTTAAAGGTA11.1904761904761905No Hit
TGTTCACCAATGACGCCCGATGCGAC11.1904761904761905No Hit
TGATGATATTATTGGAGGCACACACGAC11.1904761904761905No Hit
TTATTATGTTCGGAGAGGCTATGGCCCTGC11.1904761904761905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)