FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14072391
Sequences flagged as poor quality0
Sequence length15-35
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACCTCGGGCTGTACGGGACTACCCGG5872214.172858755843268No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCT4596413.2662608649802296No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCT3127472.222415508494612No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCC1512671.0749203884400311No Hit
GCTAATAACTCTACCGATTGACGTCACCCGCAGAA802390.5701873974365834No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAGACTC783520.5567781622895498No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAGACTCA692730.49226176276654054No Hit
GCGAAGATGGCGGAATCGGTAGACGCGCTAGC684700.4865555540632718No Hit
AACGACCTCGGGCTGTACGGGACTACCCGG658880.46820757041216376No Hit
CTCGGTAGTATAGTGGTCAGTATCCCCGCCT656130.4662533893494005No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCC605650.4303817311500227No Hit
TCCTCGTAGTATAGTGGTCAGTATCCCCGCCT590840.41985757786292316No Hit
TAGACTGTACCCCCTCTGGTCTAATGGACCGC559220.3973880486976236No Hit
GTCCGTCGGCCCCTGGC526730.37430028770519524No Hit
CTTTAACACTGAGGCGTGATGACGAGTC438570.31165279588948314No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAGA434050.3084408328335959No Hit
CGCCGGTCGTGGCTGGGAACC379490.26966988054837304No Hit
GGGGCTGATTTTGGATTCGACGAAATTT375660.26694823928641553No Hit
GCCTCCGGGCGGGACCTC371880.2642621285892355No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTCGACTG335240.23822533072027346No Hit
CTCCGGGCGGGACCTCGAATCGTC332560.23632089244819873No Hit
TAGACTGTACCCCCCCTGGTCTAATGGACCGT325830.2315384784291454No Hit
GAAGGCGACCCCCTGGACGAAGACTGACGCTC322260.22900159610403092No Hit
TAGACTGTACCCCCTCTGGTCTAATGGACCGCT298440.21207483504402347No Hit
GCTAATAACTCTACCGATTGACGTCACCCGCAGA294070.20896946368246877No Hit
AAGGAACTCCTCATACCCGTTGG290510.20643968747030977No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAG288780.20521032992900778No Hit
AACGCGAAAAACCTTACCTACTCTTGACATCC287670.204421551390947No Hit
ACACCATCGGATGAACCCAGACGGGA278990.19825344534557063No Hit
TCTTTAACACTGAGGCGTGATGACGAGTC276760.19666878215649353No Hit
TTAAAGTGGTACGCGAGCTGGGTTTAGAAC275370.1956810324556786No Hit
AGGCCTCACACCATCGGATGAACCCAGACGGGA273720.1945085238180207No Hit
TCCTCGGTAGTATAGTGGTCAGTATCCCCGCCTG273260.19418164262206755No Hit
TCGTTTCCCGGACGATGCACCA269670.19163054807104207No Hit
ACGTTTCGGGTCACGTACATCCGAGGGTCGGT253200.1799267800333291No Hit
ACGTACATCCGAGGGTCGGT250160.17776652169485624No Hit
GCATCTTTGGTTCAGTGGTAGAATGCTCGCCT246790.17537176162885185No Hit
AATCACGGAATTCTTGAACCAAGCGC238750.16965844681262765No Hit
ACGAAAGTAGAAGTATACGGTGTGACGCCTGCCC236380.1679742980421735No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAAC228310.1622396648870828No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCGGC227680.1617919797708861No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGAGAC226350.16084686674780427No Hit
GCCGGTCGTGGCTGGGAACC226230.16076159339233823No Hit
GGGGCTATAGCTCAGACGGGAGAGCGCCT225320.16011493711338748No Hit
AACGCGAAAAACCTTACCTACTCTTGACATCCA223390.15874345731297546No Hit
GCCGGTCGTGGCTGGGAACCACC214090.15213477226435793No Hit
CGCCGGTCGTGGCTGGGAACCACC213030.15138152429107463No Hit
ATGTCCTGCCGGTGGCGGTTCGGTCTGGTC211430.1502445462181942No Hit
GTCCGTCGGCCCCTG209670.14899387033802572No Hit
GTAATGATGGCCAGGCTGTCTCCACCCGA208060.14784978615218977No Hit
ACGTAACGTTGGCGTC205750.14620827405946865No Hit
TTTAACACTGAGGCGTGATGACGAGTC204820.14554740555460688No Hit
GATAAACCGGAGGAAGGCGGGGATAACGTCAAGTC204000.14496470429225566No Hit
CGCCGGTCGTGGCTGGGAACCAC193820.1377306813035539No Hit
GTCCGTCGGCCCCTGG191600.13615312422743228No Hit
ACGACCTCGGGCTGTACGGGACT191470.13606074475901075No Hit
AACGCGAAAAACCTTACCTACTCTTGACA188520.13396444143713743No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCGG187220.1330406467529221No Hit
ATGCAAGTCGAGCGGCAGCGGGGAGT185150.131569681371133No Hit
TTAGGCCTCACACCATCGGATGAACCCAGACGGGA184270.13094434343104877No Hit
GGGGACAGAACTAGGAAGCAACTTAAAAGC177960.12646038615612656No Hit
TAGGCCTCACACCATCGGATGAACCCAGACGGGA176930.12572845652170977No Hit
AATTGAGTGTAGGCGACGACAATACCAGGAGTCCC176180.12519549805004707No Hit
GCCTCCGGGCGGGACCTCGAATCGTC172400.12250938735286704No Hit
GCGGTCCGGGTGATGTCCTCTTCGGCG172320.122452538449223No Hit
GCATCGTTGGTTCAGTGGTAGAATGCTCGCCTGCC169420.12039176569212723No Hit
ACGACCTCGGGCTGTACGGGACTACCC163420.11612809791882558No Hit
TCGACTCCTTCTGCCGGCACCA162910.11576568615809496No Hit
AACGCGAAAAACCTTACCTACTCTTGACATCCAGA153460.10905040941514488No Hit
ATCACGGAATTCTTGAACCAAGCGCAAGCG149750.10641404150865336No Hit
AAGTCTCGTCCCAGGCACCA143480.10195850868555316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)