FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.mivirus.NODE_23707.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.mivirus.NODE_23707.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531
Sequences flagged as poor quality0
Sequence length22-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGGCATCAATGATCGACTTGTAGCAGG499.227871939736348No Hit
TGGCCCCACTGTCCAACTTGTTCTCTCCCT315.83804143126177No Hit
TGAGGACTTTGATGGCTGTGTACGCTAT173.2015065913371No Hit
TCTGAGAGTGACCACGAACGTTATTCGTT163.0131826741996233No Hit
TCCTTGGATTTTGACTGCTCTGTCTAACC163.0131826741996233No Hit
ATGGCCCCACTGTCCAACTTGTTCTCTCCCT152.824858757062147No Hit
TCCATTGATAGGACATGGCCCCACTGTCCAAC132.4482109227871938No Hit
TGAGGACTTTGATGGCTGTGTACGCTATAT132.4482109227871938No Hit
TATTCGTTGTTGCGGGCATGATGGACTG132.4482109227871938No Hit
TCTGAAGTACTGTCGCTCTGAGAGTGA112.0715630885122414No Hit
TCCTTGGATTTTGACTGCTCTGTCTAACCT101.8832391713747645No Hit
TATGGCATCAATGATCGACTTGTAGCAGGT101.8832391713747645No Hit
TGAGGACTTTGATGGCTGTGTACGCTATA101.8832391713747645No Hit
TATGGCATCAATGATCGACTTGTAGCAG91.694915254237288No Hit
TCGACTTGTAGCAGGTAAGGGCGCTTCCGG91.694915254237288No Hit
TGTTTCCTCCAATGCGTGATGCTGCGTT61.1299435028248588No Hit
TTCAACGTCTCTTCGCCAATGACCCATA61.1299435028248588No Hit
TCAAAATCCATTGATAGGACATGGCCCCAC61.1299435028248588No Hit
TACTTGTCGTCTCTAATTTAGGTGTTGGT61.1299435028248588No Hit
TCAATGATCGACTTGTAGCAGGTAAGGGC50.9416195856873822No Hit
TTATTCGTTGTTGCGGGCATGATGGACT50.9416195856873822No Hit
TCATGTCTTGTATGTAACCACCGGTGATA50.9416195856873822No Hit
TTATTCGTTGTTGCGGGCATGATGGACTG50.9416195856873822No Hit
TATTCGTTGTTGCGGGCATGATGGACTGT50.9416195856873822No Hit
TCCATTGATAGGACATGGCCCCACTGTCGG50.9416195856873822No Hit
TGCCCACTGTCCAACTTGTTCTCTCCCTT40.7532956685499058No Hit
TTTATGGCATCAATGATCGACTTGTAGCAG40.7532956685499058No Hit
TGATGGACTGTTTGTTGTTGTGGTTGTC40.7532956685499058No Hit
TAATCCACCACCATTGCATATTTAATTATT40.7532956685499058No Hit
TGAGAGTGACCACGAACGTTATTCGTTGTT40.7532956685499058No Hit
TGAGGACTTTGATGGCTGTGTACGCTATAA40.7532956685499058No Hit
TCGACTTGTAGCAGGTAAGGGCGCTTCCGGC40.7532956685499058No Hit
ATGGCCCCACTGTCCAACTTGTTCTCTCCCTT40.7532956685499058No Hit
TCCGGCCGCTTGATCGAGGGTCACCTTCAATTTAA40.7532956685499058No Hit
TAAGGGCGCTTCCGGCCGCTTGATCGAGG30.5649717514124294No Hit
TTACAAAAGTTTTGGGCTTCGTCAGGAT30.5649717514124294No Hit
TGCGGGCATGATGGACTGTTTGTTGTTGT30.5649717514124294No Hit
TCATAGAAGCTTTCACCCTTCCAGATATC30.5649717514124294No Hit
TGCTGCGTTACAAAAGTTTTGGGCTTCGT30.5649717514124294No Hit
TGATGAGGACTTTGATGGCTGTGTACGCTAAA30.5649717514124294No Hit
TAAGGGCGCTTCCGGCCGCTTGATCGAG30.5649717514124294No Hit
TGATCGACTTGTAGCAGGTAAGGGCGCT30.5649717514124294No Hit
TCTGAAGTACTGTCGCTCTGAGAGTG30.5649717514124294No Hit
TCAAGCTGTCCATCAAAATCCATTGATAGG30.5649717514124294No Hit
TATTCGTTGTTGCGGGCATGATGGAC30.5649717514124294No Hit
TTATGGCATCAATGATCGACTTGTAGCAG30.5649717514124294No Hit
TTCACCCTTCCAAATATCACTTCAAGCTG30.5649717514124294No Hit
TTCGTTGTTGCGGGCATGATGGACTGTT30.5649717514124294No Hit
TCTTGTATGTAACCACCGGTGATATGG30.5649717514124294No Hit
TTGTATCCTGTAGATCGTACAATCCCCA30.5649717514124294No Hit
AATTCAACGTCTCTTCGCCAATGACCCAT30.5649717514124294No Hit
TCCGGCCGCTTGATCGAGGGTCACCTTCATCA30.5649717514124294No Hit
GAGGACTTTGATGGCTGTGTACGCTATAT20.3766478342749529No Hit
TCTGAAGTACTGTCGCTCTGAGAGTGC20.3766478342749529No Hit
TACATGTTTCCTCCAATGCGTGATGCTGCG20.3766478342749529No Hit
TGCGTGATGCTGCGTTACAAAAGTTTTGGGC20.3766478342749529No Hit
TGCATATTTCGTCATAGCTTTCGTCGTTT20.3766478342749529No Hit
TTGATGAGGACTTTGATGGCTGTGTACG20.3766478342749529No Hit
TACATAATCCACCACCATTGCATATTTAATT20.3766478342749529No Hit
TGACCACGAACGTTATTCGTTGTTGCGGGC20.3766478342749529No Hit
TGATGGACTGTTTGTTGTTGTGGTTGT20.3766478342749529No Hit
TGCATATTTCGTCATAGCTTTCGTCGTTTT20.3766478342749529No Hit
TCACCCTTCCAGATATCACTTCAAGCTGTC20.3766478342749529No Hit
TGGCATCAATGATCGACTTGTAGCAGG20.3766478342749529No Hit
TGTCCAACTTGTTCTCTCCCTTCTTGATG20.3766478342749529No Hit
TCGTCTCTAATTTAGGTGTTGGTCTTTT20.3766478342749529No Hit
TATCCTGTAGATCGTACAATCCCCAAGTT20.3766478342749529No Hit
TGTCTTGTATGTAACCACCGGTGATATGG20.3766478342749529No Hit
TCTGAAGTACTGTCGCTCTGAGAGTGACCAC20.3766478342749529No Hit
TGATGAGGACTTTGATGGCTGTGTACGC20.3766478342749529No Hit
TCAATGATCGACTTGTAGCAGGTAAGG20.3766478342749529No Hit
TGGCCCCACTGTCCAACTTGTTCTCTCCCTT20.3766478342749529No Hit
TAGACACTCATGTCTTGTATGTAACCACCGGT20.3766478342749529No Hit
TCTTCGCCAATGACCCATATTTAGCACCT20.3766478342749529No Hit
TCCTCCAATGCGTGATGCTGCGTTACAAAAG20.3766478342749529No Hit
TCGACTTTTAGCAGGTAAGGGCGCTTCCGG10.18832391713747645No Hit
TACATGTTTCCTCCAATGCGTGATGCTGCT10.18832391713747645No Hit
TATTTCGTCATAGCTTTCGTCGTTTGT10.18832391713747645No Hit
TACAAAAATTCAACGTCTCTTCGCCAATGACCC10.18832391713747645No Hit
TACCCAAGCTCCTTGGATTTTGACTGCTC10.18832391713747645No Hit
TGTAGCAGGTAAGGGCGCTTCCGGCCGC10.18832391713747645No Hit
ATGGCCCCACTGTCCAACTTGTTCTCTCC10.18832391713747645No Hit
CCACCGGTGATATGGTTATAATTCTGTAC10.18832391713747645No Hit
TCGTCATAGCTTTCGTCGTTTGTTGTAC10.18832391713747645No Hit
TCTGAAGTACTGTCGCTCTGAGAGTGACCACGAAC10.18832391713747645No Hit
TCTGGAAGATTACCCAAGCTCCTTGGAT10.18832391713747645No Hit
GGCGCTTCCGGCCGCTTGATCGAGGGTCACC10.18832391713747645No Hit
TCAACGTCTCTTCGCCAATGACCCATATT10.18832391713747645No Hit
TGTTGCGGGCATGATGGACTGTTTGTTG10.18832391713747645No Hit
TTTCACCCTTCCAGATATCACTTCAAGCTG10.18832391713747645No Hit
TGGACTGTTTGTTGTTGTGGTTGTCGATA10.18832391713747645No Hit
TCATAGAAGCTTTCACCCTTCCAGATATCAC10.18832391713747645No Hit
ATCACTTCAAGCTGTCCATCAAAATCCATT10.18832391713747645No Hit
TTGTTGCGGGCATGATGGACTGTTTGTTG10.18832391713747645No Hit
TCGTTGTTGCGGGCATGATGGACTGTTT10.18832391713747645No Hit
ATGGCCCCACTGTCCAACTTGTTCTCTCCC10.18832391713747645No Hit
TTCCGGCCGCTTGATCGAGGGT10.18832391713747645No Hit
TAGAAGCTTTCACCCTTCCAGATATCACT10.18832391713747645No Hit
TTGTAGCAGGTAAGGGCGCTTCCGGCCGCA10.18832391713747645No Hit
TATGGTTATAATTCTGTACATACAGTGGT10.18832391713747645No Hit
TACGTTGTTGCGGGCATGATGGACTGTT10.18832391713747645No Hit
TGCCCACTGTCCAACTTGTTCTCTCCCTTC10.18832391713747645No Hit
TACAAAAATTCAACGTCTCTTCGCCAATGA10.18832391713747645No Hit
TCGCTCTGAGAGTGACCACGAACG10.18832391713747645No Hit
TCACCCTTCCAAATATCACTTCAAGCTG10.18832391713747645No Hit
TCTGAAGTACTGTCGCTCTGAGAGTGAA10.18832391713747645No Hit
TTTATGGCATCAATGATCGACTTGTAGCA10.18832391713747645No Hit
TCGCTCTGAGAGTGACCACGAACGTTAT10.18832391713747645No Hit
ATGGCATCAATGATCGACTTGTAGCA10.18832391713747645No Hit
TTTTATGGCATCAATGATCGACTTGTAGC10.18832391713747645No Hit
TCCAAGCTGTCCATCAAAATCCATTGATA10.18832391713747645No Hit
TTCGTTGTTGCGGGCATGATGGACTGT10.18832391713747645No Hit
TGGCCCCACTGTCCAACTTGTTCTCTCC10.18832391713747645No Hit
TCATGTCTTGTATGTAACCACCGGTGA10.18832391713747645No Hit
TAAGGGCGCTTCCGGCCGCTTGATCGAGGGTCA10.18832391713747645No Hit
TTCACCCTTCCAAATATCACTTCAAGCTGTCC10.18832391713747645No Hit
TACATGTTTCCTCCAATGCGTGATGCTGCGT10.18832391713747645No Hit
GAGGACTTTGATGGCTGTGTACGCTATA10.18832391713747645No Hit
TGCCCACTGTCCAACTTGTTCTCTCCCT10.18832391713747645No Hit
TGGCCCCACTGTCCAACTTGTTCTCTCCCG10.18832391713747645No Hit
CCCAAGCTCCTTGGATTTTGACTGCT10.18832391713747645No Hit
CCTTGGATTTTGACTGCTCTGTCTAACC10.18832391713747645No Hit
TCTGAAGTACTGTCGCTCTGAGAGTGACC10.18832391713747645No Hit
TCTCTTCGCCAATGACCCATATTTAGCACC10.18832391713747645No Hit
GGCCCCACTGTCCAACTTGTTCTCTCCC10.18832391713747645No Hit
TAGACACTCATGTCTTGTATGTAACCACCGG10.18832391713747645No Hit
CTCCTTGGATTTTGACTGCTCTGTCTAACA10.18832391713747645No Hit
CCAAGCTGTCCATCAAAATCCATTGATAG10.18832391713747645No Hit
TGAGGACTTTGATGGCTGTGTACGCTATATT10.18832391713747645No Hit
TGGCCCCACTGTCCAACTTGTTCTTTCCCTT10.18832391713747645No Hit
TCGTCATAGCTTTCGTCGTTTGTTGTACT10.18832391713747645No Hit
TCACTTCAAGCTGTCCATCAAAATCCATT10.18832391713747645No Hit
TTGATAGGACATGGCCCCACTGTC10.18832391713747645No Hit
TGATGGACTGTTTGTTGTTGTGGTTGTCT10.18832391713747645No Hit
TCGACTTGTAGCAGGTAAGGGCGCTTCCA10.18832391713747645No Hit
TCGACTTGTAGCAGGTAAGGGCGCTTCCG10.18832391713747645No Hit
TGATGAGGACTTTGATGGCTGTGTACGCTATT10.18832391713747645No Hit
TAGTACATGTTTCCTCCAATGCGTGATGC10.18832391713747645No Hit
TCGTCATAGCTTTCGTCGTTTGTTGTACTA10.18832391713747645No Hit
TGCGGCGTTACAAAAGTTTTGGGCTTCGT10.18832391713747645No Hit
TATTTCGTCATAGCTTTCGTCGTTTGTT10.18832391713747645No Hit
TGGACTGTTTGTTGTTGTGGTTGTCGAT10.18832391713747645No Hit
CTCATGTCTTGTATGTAACCACCGGTGAT10.18832391713747645No Hit
TGTATCCTGTAGATCGTACAATCCCCAAGT10.18832391713747645No Hit
TCCAACTTGTTCTCTCCCTTCTTGATGA10.18832391713747645No Hit
TTCAACGTCTCTTCGCCAATGACCCATAT10.18832391713747645No Hit
TATTCGTTGTTGCGGGCATGATGGACT10.18832391713747645No Hit
TTCACCCTTCCAGATATCACTTCAAGCTG10.18832391713747645No Hit
CTGGAAGATTACCCAAGCTCCTTGGAT10.18832391713747645No Hit
TCATACAAAAATTCAACGTCTCTTCGCCAAT10.18832391713747645No Hit
TTGTAGCAGGTAAGGGCGCTTCCGGC10.18832391713747645No Hit
TAACCACCGGTGATATGGTTATAATTCTGTACAAC10.18832391713747645No Hit
TATGGCATCAATGATCGACTTGTAGCA10.18832391713747645No Hit
TCTTCGCCAATGACCCATATTTAGCACCTT10.18832391713747645No Hit
AAATTCAACGTCTCTTCGCCAATGACCCA10.18832391713747645No Hit
TATTCGTTGTTGCGGGCATGATGGACTGTT10.18832391713747645No Hit
TAGCAGGTAAGGGCGCTTCCGGCCGCT10.18832391713747645No Hit
ATTCGTTGTTGCGGGCATGATGGACTGT10.18832391713747645No Hit
ATGGCCCCACTGTACAACTTGTTCTCTCCCT10.18832391713747645No Hit
TTCAACGTCTCTTCGCCAATGACCCATATTT10.18832391713747645No Hit
AATTCAACGTCTCTTCGCCAATGACCCATA10.18832391713747645No Hit
TGTCCAACTTGTTCTCTCCCTTCTTGATGAAAAA10.18832391713747645No Hit
CGACTTGTAGCAGGTAAGGGCGCTTCCGG10.18832391713747645No Hit
TGTTTCCTCCAATGCGTGATGCTGCGTTAC10.18832391713747645No Hit
TCTGAAGTACTGTCGCTCTGAGAGT10.18832391713747645No Hit
CCCCACTGTCCAACTTGTTCTCTC10.18832391713747645No Hit
CCATTGATAGGACATGGCCCCACTGTCG10.18832391713747645No Hit
TCCATTGATAGGACATGGCCCCACTGTCCAA10.18832391713747645No Hit
TCACCCTTCCAAATATCACTTCAAGCTGTC10.18832391713747645No Hit
TCAACGTCTCTTCGCCAATGACCCATA10.18832391713747645No Hit
TGATGAGGACTTTGATGGCTGTGTACGCTC10.18832391713747645No Hit
ATGGCATCAATGATCGACTTGTAGCAGGT10.18832391713747645No Hit
ACCCTTCCAAATATCACTTCAAGCTGTCC10.18832391713747645No Hit
TCTGAAGTACTGTCGCTCGGAGAGTGACCACGAAC10.18832391713747645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)