FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.mivirus.NODE_8525.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.mivirus.NODE_8525.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373
Sequences flagged as poor quality0
Sequence length16-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGAGAACTTTGACTGCGCTATAGGCT16812.235979606700656No Hit
TATGTATTTCCTCCTATCCTTGCGGCGGC584.224326292789512No Hit
TCTTACCGATTGTCTTGTCAGCTTCTTCAGC392.8404952658412235No Hit
TTCACGAGAACTTTGACTGCGCTATAGGCT372.69482884195193No Hit
TAAGCAGACTCCACAGCTGGTAGATGGGC352.5491624180626364No Hit
TATTGTCTTCAGTCCCTTCTCCAAGCACC332.4034959941733427No Hit
TCAGCAGCTGTAGCATCTATTGTTCGAATGGC302.1849963583394025No Hit
TGAGAGAATTTGGAAACCAGTTGGGCGC271.9664967225054626No Hit
TCCTTGCGGCGGCGTTACAGAAGCTCTGTGC251.820830298616169No Hit
TGAGAGAATTTGGAAACCAGTTGGGCGCA221.602330662782229No Hit
TGGGAATCCTACAGCCTTGAGGTCGGCT161.1653313911143481No Hit
TGTGAAATCTCCCGTACATCAGCCTGACACT161.1653313911143481No Hit
CACGTCCAGGGCACAGAGCATTATTGAAAT161.1653313911143481No Hit
TGTGAAATCTCCCGTACATCAGCCTGACAC151.0924981791697013No Hit
CACGAGAACTTTGACTGCGCTATAGGCT151.0924981791697013No Hit
TCACGAGAACTTTGACTGCGCTATAGGC141.0196649672250546No Hit
TATTTAGCCAAATCAGAGCCAAGTCCCATT141.0196649672250546No Hit
TTTCACGAGAACTTTGACTGCGCTATAGGC130.9468317552804079No Hit
TAGTCTATTGTCTTCAGTCCCTTCTCCA120.8739985433357611No Hit
TGGGACCATAGGAGAGGGCTGAGATACAGCT120.8739985433357611No Hit
TACCGATTGTCTTGTCAGCTTCTTCAGCC110.8011653313911145No Hit
TCCTAAATTCCCCTGATTTAAGTACTGCT110.8011653313911145No Hit
TGTTTCACGAGAACTTTGACTGCGCTATA110.8011653313911145No Hit
TAACGTAGGATGATATGGCTCGGAAAGTA110.8011653313911145No Hit
TTGTTGTGAAATCTCCCGTACATCAGCCT90.6554989075018208No Hit
TAGTCTATTGTCTTCAGTCCCTTCTCCAA90.6554989075018208No Hit
TCTTCTGGTCGCGTAGTGGCTCTTCTCCC80.5826656955571741No Hit
TTGGGCGCAAACGTTTTGGGCTGGGAATCC80.5826656955571741No Hit
TAGGGATCGTGTAGTCTATTGTCTTCAGTC80.5826656955571741No Hit
TTGGCTCCCTTATGTTTCACGAGAACTTTGAC80.5826656955571741No Hit
TTCACGAGAACTTTGACTGCGCTATAGGCTA70.5098324836125273No Hit
TGCACTACCAGCGGCCTGATCTAATGTCACC70.5098324836125273No Hit
TGTAGTCTATTGTCTTCAGTCCCTTCTCC60.43699927166788055No Hit
TGGGACCATAGGAGAGGGCTGAGATACAGC60.43699927166788055No Hit
TATGGCTCGGAAAGTATGTCCAATGACTT60.43699927166788055No Hit
GGCGCAAACGTTTTGGGCTGGGAATCCTAC60.43699927166788055No Hit
TATTTAGCCAAATCAGAGCCAAGTCCCAT50.3641660597232338No Hit
TCACGAGAACTTTGACTGCGGTATAGGCT50.3641660597232338No Hit
TTCTTCTGGTCGCGTAGTGGCTCTTCTCT50.3641660597232338No Hit
TGTGAAATCTCCCGTACATCAGCCTGAC50.3641660597232338No Hit
TTCACGAGAACTTTGACTGCGCTATAGGC50.3641660597232338No Hit
TGGCTCGGAAAGTATGTCCAATGACTTGTT50.3641660597232338No Hit
TTCGGCCACAAGGATAGGGATCGTGTAGTC50.3641660597232338No Hit
TTAGCCAAATCAGAGCCAAGTCCCAT50.3641660597232338No Hit
TGTCTGGCCGTGTTGGGATCCCTTTGTAG50.3641660597232338No Hit
TAGATCTCACGACACCTAAATTACGGGCG50.3641660597232338No Hit
TCCCATTGTCTCCAGGGTTACTTGCCTCCC50.3641660597232338No Hit
TCTCCAAGCACCGACCCACTGATACAGTA50.3641660597232338No Hit
TTGGGCGCAAACGTTTTGGGCTGGGAA50.3641660597232338No Hit
TTAACGTAGGATGATATGGCTCGGAAAGTA50.3641660597232338No Hit
TACCGATTGTCTTGTCAGCTTCTTCAGCCAC50.3641660597232338No Hit
TCGAGAAGCCTCCGATTCCCTCACTGAG50.3641660597232338No Hit
TCACGAGAACTTTGACTGCGCTATAGG50.3641660597232338No Hit
TCAGCCACTAAAAAGCACTCATCCGGTATAAG50.3641660597232338No Hit
TTGTCTTCAGTCCCTTCTCCAAGCACCGAC40.29133284777858703No Hit
TCATCAGGATAAAGAGCAAATATAACCGCC40.29133284777858703No Hit
TTCGGCCACAAGGATAGGGATCGTGTAGT40.29133284777858703No Hit
TCTGGTCGCGTAGTGGCTCTTCTCCCAC40.29133284777858703No Hit
TTGGCAACAGGCGGGGTTGTGACTGAACT40.29133284777858703No Hit
CACGAGAACTTTGACTGCGCTATAGGCTA40.29133284777858703No Hit
CCAGGGCACAGAGCATTATTGAAATTGT40.29133284777858703No Hit
TCCAGGGCACAGAGCATTATTGAAATTGTC40.29133284777858703No Hit
TGAGACTTACTCTAACGCCGCTACAGG40.29133284777858703No Hit
TGTCTTCAGTCCCTTCTCCAAGCACCGAC40.29133284777858703No Hit
TCCTATCCTTGCGGCGGCGTTACAGAAGC40.29133284777858703No Hit
TTTGGCAACAGGCGGGGTTGTGACTGAA40.29133284777858703No Hit
TGGGAATCCTACAGCCTTGAGGTCGG40.29133284777858703No Hit
TAGATGGGCTAACCCACAGTGTGGAC40.29133284777858703No Hit
TACATATTTCCTCCTATCCTTGCGGCGGC40.29133284777858703No Hit
TGGGACCATAGGAGAGGGCTGAGATACAGT40.29133284777858703No Hit
TTAGCCAAATCAGAGCCAAGTCCCATTGTC40.29133284777858703No Hit
TGGTAGATGGGCTAACACACAGTGTGGACAT40.29133284777858703No Hit
TCACGAGAACTTTGACTGCGCTATAGGCTC40.29133284777858703No Hit
TCTCGCATTGCCATTCTTTTTGTAGAAGCT40.29133284777858703No Hit
TGCACTACCAGCGGCCTGATCTAATGTC40.29133284777858703No Hit
TTGGCGAACAGATCTTTTGCGTTACTCTCAT40.29133284777858703No Hit
TCCACAAAATATTTTTAAAATACCATCGCTG40.29133284777858703No Hit
TTCTGTCTGTAGATCTCACCTGGATTCCT40.29133284777858703No Hit
TGGGTTCTTCTGGTCGCGTAGTGGCTCTT30.21849963583394028No Hit
TCTCCCGTACATCAGCCTGACACTCATCGG30.21849963583394028No Hit
TTCTTCTGGTCGCGTAGTGGCTCTTCTCCC30.21849963583394028No Hit
TGTTGTGAAATCTCCCGTACATCAGCCT30.21849963583394028No Hit
TCCCCTGATTTAAGTACTGCTGTTTCTGT30.21849963583394028No Hit
TCCCCTGATTTAAGTACTGCTGTTTCTGTC30.21849963583394028No Hit
TATAGACCGACTCTTTCTCTGGGCATGTC30.21849963583394028No Hit
TCGAGAAGCCTCCGATTCCCTCACTGAGT30.21849963583394028No Hit
CACGTCCAGGGCACAGAGCATTATTGAAA30.21849963583394028No Hit
TAGTCTATTGTCTTCAGTCCCTTCTCCAAG30.21849963583394028No Hit
TCCAGGGCACAGAGCATTATTGAAATTGT30.21849963583394028No Hit
TGAGCAGAAGCAGGGACCCTGATGTCAGC30.21849963583394028No Hit
TGGGCGCAAACGTTTTGGGCTGGGAATCCT30.21849963583394028No Hit
TTGGGCGCAAACGTTTTGGGCTGGGAAT30.21849963583394028No Hit
TCAGCAGGTGGTGTGAATGTATCGATGAT30.21849963583394028No Hit
TGCACTACCAGCGGCCTGATCTAATGTCAC30.21849963583394028No Hit
TTGAGAGAATTTGGAAACCAGTTGGGCGC30.21849963583394028No Hit
TGTCTTCAGTCCCTTCTCCAAGCACCGA30.21849963583394028No Hit
TCTCCAAGCACCGACCCACTGATACAGTAC30.21849963583394028No Hit
TTTTATAGCCGGTAGATCTCACGACACCT30.21849963583394028No Hit
TAGATCTCACGACACCTAAATTACGGGAG30.21849963583394028No Hit
TGGGAATCCTACAGCCTTGAGGTCGGCTC30.21849963583394028No Hit
TTGGGCGCAAACGTTTTGGGCTGGGAT30.21849963583394028No Hit
TCACGAGAACTTTGACTGCGCTATAGGCG30.21849963583394028No Hit
TCGAGAAGCCTCCGATTCCCTCACTG30.21849963583394028No Hit
TATTTTTATAGCCGGTAGATCTCACGAC30.21849963583394028No Hit
TCGGTAATTTGACGAGAGATAGTCCCTTGT30.21849963583394028No Hit
TCCAAGCACCGACCCACTGATACAGTACAGG30.21849963583394028No Hit
TCCAATGACTTGTTGTGAAATCTCCCGTAC30.21849963583394028No Hit
CGTGAAATCTCCCGTACATCAGCCTGACACT30.21849963583394028No Hit
TTGTGAAATCTCCCGTACATCAGCCTGA30.21849963583394028No Hit
TTCTGGTCGCGTAGTGGCTCTTCTCCCACA30.21849963583394028No Hit
CAGGGCACAGAGCATTATTGAAATTGTC30.21849963583394028No Hit
TCCTATTAACGTAAGCAGACTCCACAGCT30.21849963583394028No Hit
TTGTGAGACTTACTCTAACGCCGCTACAGG20.14566642388929352No Hit
CTCCAAGCACCGACCCACTGATACAGTACA20.14566642388929352No Hit
TAGCAAGCCACATAAGCCAGAGCCGCTGGG20.14566642388929352No Hit
ACGTCCAGGGCACAGAGCATTATTGAAAT20.14566642388929352No Hit
TGACTTGTTGTGAAATCTCCCGTACATCAGCCT20.14566642388929352No Hit
CTGGGAATCCTACAGCCTTGAGGTCGGCT20.14566642388929352No Hit
TTCAGTCCCTTCTCCAAGCACCGACCCACT20.14566642388929352No Hit
TTAGCCAAATCAGAGCCAAGTCCCATTGTCTCCAG20.14566642388929352No Hit
TCATTTCTCGGAAAGGGTACAGCAGGTC20.14566642388929352No Hit
TCTGGTCGCGTAGTGGCTCTTCTCCCACG20.14566642388929352No Hit
TAGGAGAGGGCTGAGATACAGCTTTTGGCAAC20.14566642388929352No Hit
TATCCTTGCGGCGGCGTTACAGAAGCTCT20.14566642388929352No Hit
TAGCAAGCCACATAAGCCAGAGCCGCTGGGT20.14566642388929352No Hit
TTGTCTCCAGGGTTACTTGCCTCCCTCCTAG20.14566642388929352No Hit
TGTTCTTCCAATTACGATCTACTACGTGA20.14566642388929352No Hit
TGATGAGGCTTGTGGGACCATAGGAGAGG20.14566642388929352No Hit
TATGTCCAATGACTTGTTGTGAAATCTCCCGT20.14566642388929352No Hit
TAGACCGACTCTTTCTCTGGGCATGTCTGCT20.14566642388929352No Hit
TTCTTCTGGTCGCGTAGTGGCTCTTCTCC20.14566642388929352No Hit
TTGGTAGCCACGTCCAGGGCACAGGGCA20.14566642388929352No Hit
TGAGGCTTGTGGGACCATAGGAGAGGGC20.14566642388929352No Hit
TTGGGCGCAAACGTTTTGGGCTGGGAATC20.14566642388929352No Hit
TGTAGTCTATTGTCTTCAGTCCCTTCT20.14566642388929352No Hit
TGAGGAGGGAGGTATCTCGCATTGCCATT20.14566642388929352No Hit
TAAAAAAGGCACACTTTTCTGAAACACCCA20.14566642388929352No Hit
TCTATTGTCTTCAGTCCCTTCTCCAAGC20.14566642388929352No Hit
TATATCGGAAACAGTACACCCTACAGGAAAT20.14566642388929352No Hit
TGTATTTCCTCCTATCCTTGCGGCGGC20.14566642388929352No Hit
TAGCCGATATGAGCCTCAGCAGCTGTAGC20.14566642388929352No Hit
TGCGGCGGCGTTACAGAAGCTCTGTGCTT20.14566642388929352No Hit
TGGGACCATAGGAGAGGGCTGAGATACAG20.14566642388929352No Hit
TATTTGGCTCCCTTATGTTTCACGAGAAC20.14566642388929352No Hit
TTTTATAGCCGGTAGATCTCACGACAC20.14566642388929352No Hit
TATGGCTCGGAAAGTATGTCCAATGACTTGT20.14566642388929352No Hit
TTGGGCGCAAACGTTTTGGGCTGGGAATCCT20.14566642388929352No Hit
ATTGTCTTCAGTCCCTTCTCCAAGCACC20.14566642388929352No Hit
GACCCACTGATACAGTACAGGATGTCTGCTG20.14566642388929352No Hit
TTTGGCAACAGGCGGGGTTGTGACT20.14566642388929352No Hit
TCTATTGTCTTCAGTCCCTTCTCCAAGCACC20.14566642388929352No Hit
CTGGTCGCGTAGTGGCTCTTCTCCCACGTT20.14566642388929352No Hit
TGGGCGCAAACGTTTTGGGCTGGGAATCCTACA20.14566642388929352No Hit
TGAGTTTGGCGAACAGATCTTTTGCGTTACT20.14566642388929352No Hit
TGCGGCGGCGTTACAGAAGCTCTGTGCTTCTCT20.14566642388929352No Hit
TTGGAAACCAGTTGGGCGCAAACGTTTTG20.14566642388929352No Hit
ATTGTCTTCAGTCCCTTCTCCAAGCACCC20.14566642388929352No Hit
TCGAGAAGCCTCCGATTCCCTCACTGAAA20.14566642388929352No Hit
TTTTGGCAACAGGCGGGGTTGTGAC20.14566642388929352No Hit
TCATTTCTCGGAAAGGGTACAGCAGGTCC20.14566642388929352No Hit
TTCTGGTCGCGTAGTGGCTCTTCTCCCAC20.14566642388929352No Hit
TAGGATGATATGGCTCGGAAAGTATGTC20.14566642388929352No Hit
TAGTCTATTGTCTTCAGTCCCTTCTCC20.14566642388929352No Hit
TGTATTTCCTCCTATCCTTGCGGCGGCGTT20.14566642388929352No Hit
TGGGAATCCTACATCCTTGAGGTCGGCT20.14566642388929352No Hit
TGAGTTTGGCGAACAGATCTTTTGCGTT20.14566642388929352No Hit
CACCGACCCACTGATACAGTACAGGATG20.14566642388929352No Hit
TGACTTGTTGTGAAATCTCCCGTACATCA20.14566642388929352No Hit
TCTCGGAAAGGGTACAGCAGGTC20.14566642388929352No Hit
TCTATTGTCTTCAGTCCCTTCTCCAAGCAC20.14566642388929352No Hit
TAAAGAGCAAATATAACCGCCCAAGGGAA20.14566642388929352No Hit
TATGTTTCACGAGAACTTTGACTGCGCT20.14566642388929352No Hit
TCGTGTAGTCTATTGTCTTCAGTCCCTTC20.14566642388929352No Hit
TATAGCCGGTAGATCTCACGACACCT20.14566642388929352No Hit
TTGGTAGCCACGTCCAGGGCACAGGGCATT20.14566642388929352No Hit
CACGAGAACTTTGACTGCGCTATAGGCA20.14566642388929352No Hit
TGGGCGCAAACGTTTTGGGCTGGGAATCCTAC20.14566642388929352No Hit
TACATGGCGGCCACTAATATACACGAAAGT20.14566642388929352No Hit
TAGTCTATTGTCTTCAGTCCCTTCTCCAAGCACC20.14566642388929352No Hit
TATCAGCCCTGTCTGGCCGTGTTGGGATC20.14566642388929352No Hit
TTTTTATAGCCGGTAGATCTCACGACACC20.14566642388929352No Hit
TGAGGCTTGTGGGACCATAGGAGAGG20.14566642388929352No Hit
TATATCAGCCCTGTCTGGCCGTGTTGGGAT20.14566642388929352No Hit
TCTTACCGATTGTCTTGTCAGCTTCTTCAG20.14566642388929352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)