FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.mivirus.NODE_8525.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.mivirus.NODE_8525.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length15-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGATCAAGTAGGTATTTGTGTTTTCTCTGGC47.8431372549019605No Hit
TCGATGGAAATGGAGCCGCCT47.8431372549019605No Hit
AATGGAGCCGCCTGTAGC35.88235294117647No Hit
TCAGCCCTCTCCTATGGTCCCACAAGCCT35.88235294117647No Hit
AGCGGCGTTAGAGTAAGTCTCACAAGA35.88235294117647No Hit
TAGACTACACGATCCCTATCCTTGTGGCCT23.9215686274509802No Hit
TTCTATAGTATTTGGTGATCAAGTAGGT23.9215686274509802No Hit
TTCAATAATGCTCTGTGCCCTGGACGTG23.9215686274509802No Hit
AGGGTGTTTCAGAAAAGTGTG23.9215686274509802No Hit
TTCAGTCACAACCCCGCCTGTTGCCAAAA23.9215686274509802No Hit
ATTTCAATAATGCTCTGTGCCCTGGACGT23.9215686274509802No Hit
TACCGGATGAGTGCTTTTTAGTGGCT23.9215686274509802No Hit
TTCAATAATGCTCTGTGCCCTGGACGTGG23.9215686274509802No Hit
TTAGGACCTGCTGTACCCTTTCCGAGAA11.9607843137254901No Hit
CTGTTGCCAAAAGCTGTATCTCAGCCCTCT11.9607843137254901No Hit
CGTAGGGTGTACTGTTTCCGAT11.9607843137254901No Hit
GTAACCCCGCCTGTTGCC11.9607843137254901No Hit
AACGATCCGAAGATAACAGCAGACATCCT11.9607843137254901No Hit
TTCAGTCACAACCCCGCCTGTTGCCAAAAG11.9607843137254901No Hit
TCAATAATGCTCTGTGCCCTGGACGTGGCTACC11.9607843137254901No Hit
TCGGTCTATATAATGTGATACAGGATG11.9607843137254901No Hit
ATTTCAATAATGCCCTGTGCCCTGGACGT11.9607843137254901No Hit
ATGGAGCCGCCTGTAGC11.9607843137254901No Hit
AACGATCCGAAGATAACAGCAGACATCCTGTACT11.9607843137254901No Hit
TTGGTGATCAAGTAGGTATTTGTGTTTTCTCTGG11.9607843137254901No Hit
ATGGAGCCGCCTGTA11.9607843137254901No Hit
ACCTGCTGTACCCTTTCCGAGAAATGA11.9607843137254901No Hit
GTATGACCAACGTGGGAGAAGAGCCACT11.9607843137254901No Hit
TCACACCACCTGCTGACATCAGGGTCCCTG11.9607843137254901No Hit
ACCTGCTGTACCCTTTCCGAGAAATGATGAC11.9607843137254901No Hit
TACAGGGTGTTTCAGAAAAGTGTGCCT11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)