FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.mivirus.NODE_9891.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.mivirus.NODE_9891.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367
Sequences flagged as poor quality0
Sequence length17-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTCTCCCACAAACGTCACCACGTGACTG5113.896457765667575No Hit
CTAGACCCCGGTTAGTAGTGTACATGCCT164.35967302452316No Hit
TGAGGACGTCGAGTTAACTTTAATGTTGGT154.087193460490464No Hit
TCCCCAATTGTTCCAAGATCGGTCTCAGAG133.5422343324250685No Hit
TGGTCTCCCACAAACGTCACCACGTGACTGT133.5422343324250685No Hit
TTGGTCTCCCACAAACGTCACCACGTGAC102.7247956403269753No Hit
TCAGAGAATGAGGACGTCGAGTTAACTT102.7247956403269753No Hit
TTGGATATGTTAGCTACGCCTTGGTACAAT92.452316076294278No Hit
TCAGAGAATGAGGACGTCGAGTTAACTTT92.452316076294278No Hit
TCTACTCCTGAGCACTCTTTGTCCCCAATT92.452316076294278No Hit
TCGGTATCTTGGATATGTTAGCTACGCCT82.17983651226158No Hit
TAAAGTTATCCAGGAACCATGTTAGGGC82.17983651226158No Hit
TCATCTCTAACTAACGAGGCACCGTGGGG71.9073569482288828No Hit
TAGCACCTAGATTCCTGATTACCCACTCCC61.6348773841961852No Hit
TCTGTTTGCTATGTTCCTCTCCATGTACA61.6348773841961852No Hit
TCAACAGTGACAACCACTCGATCATTCGGT51.3623978201634876No Hit
TTGGTCTCCCACAAACGTCACCACGTGACT51.3623978201634876No Hit
TTGGATATGTTAGCTACGCCTTGGTACAA41.08991825613079No Hit
TAACACTAGTAGATCCCATAGTATGGCTA41.08991825613079No Hit
TGGTCTCCCACAAACGTCACCACGTGACT41.08991825613079No Hit
TCGGAATCTCATCGTAGCAGCTATTTGTGG30.8174386920980926No Hit
TCTTGGATATGTTAGCTACGCCTTGGTAC30.8174386920980926No Hit
CTTGGATATGTTAGCTACGCCTTGGTAC30.8174386920980926No Hit
TGCGATCTGTCACCTTGGAGCTTAATCCAG30.8174386920980926No Hit
TTCCAAGATCGGTCTCAGAGAATGAGGAC30.8174386920980926No Hit
TCTGTTTGCTATGTTCCTCTCCATGTACAC30.8174386920980926No Hit
TTGGTCTCCCACAAACGTCACCACGTGACTC30.8174386920980926No Hit
TATTGTGCGATCTGTCACCTTGGAGCTT30.8174386920980926No Hit
TTGAATAACAGCCGTCCTTTCTGCAGGAA30.8174386920980926No Hit
TCACCAGTTCTGTTTGCTATGTTCCTCTCC30.8174386920980926No Hit
TGATACAAATCACCAGTTCTGTTTGCTATG30.8174386920980926No Hit
TGATGTCACACTGTATTGAGGGGGAAGG30.8174386920980926No Hit
TGTCCCCAATTGTTCCAAGATCGGTCTCAGA30.8174386920980926No Hit
TTTGAATAACAGCCGTCCTTTCTGCAGGA20.544959128065395No Hit
TTCTGAAACTATGTCCCCATAAACCCAGG20.544959128065395No Hit
TAACAGCCGTCCTTTCTGCAGGAA20.544959128065395No Hit
TTTGTCCCCAATTGTTCCAAGATCGGTCTCA20.544959128065395No Hit
TGGTCTCCCACAAACGTCACCACGTG20.544959128065395No Hit
GATTCGGTCCACATAAG20.544959128065395No Hit
TCTGAAACTATGTCCCCATAAACCCAGG20.544959128065395No Hit
TGGTCTCCCACAAACGTCACCACGTGACTG20.544959128065395No Hit
AGGAATTGATGTCACACTGTATTGAGGG20.544959128065395No Hit
TATAAAGGAGGAGTGAGTGGGTTGCATGG20.544959128065395No Hit
TCACAATATCTCCGTGTAAACATCTGATA20.544959128065395No Hit
TTGTCCCCAATTGTTCCAAGATCGGTCTCAG20.544959128065395No Hit
TAAGTTATCCAGGAACCATGTTAGGGCCA20.544959128065395No Hit
TTGGTCTCCCACAAACGTCACCACGTGACTGT20.544959128065395No Hit
AACCATGTTAGGGCCAAATCTGATTCG20.544959128065395No Hit
TCACCAGTTCTGTTTGCTATGTTCCTCTC20.544959128065395No Hit
TGCGATAGTAGACCATAAACCTAAAATTA20.544959128065395No Hit
CCTGATTACCCACTCCCACCATCCGTAAA20.544959128065395No Hit
GAGGACGTCGAGTTAACTTTAATGTTGGT20.544959128065395No Hit
TTATCCAGGAACCATGTTAGGGC20.544959128065395No Hit
TCTCCGTGTAAACATCTGATACAAATCACC20.544959128065395No Hit
CCAGGAACCATGTTAGGGCCAAATCTGATTCGGT20.544959128065395No Hit
TGATGTCACACTGTATTGAGGGGGAAGGC20.544959128065395No Hit
TGTCCCCAATTGTTCCAAGATCGGTCTCAG20.544959128065395No Hit
TTGGCTATCTTCTTTTCCTGAGCTCGTTTC10.2724795640326975No Hit
TGTAAGCCGGCACCGCATGAGAAAACGCC10.2724795640326975No Hit
TGTGCGATCTGTCACCTTGGAGCTTAATCC10.2724795640326975No Hit
TTGTCCCCAATTGTTCCAAGATCGGTCTCA10.2724795640326975No Hit
TTGGATATGTTAGCTACGCCTTCGTACAAT10.2724795640326975No Hit
TCCCCTTTTTCATCGCTGTATATCCTACCTTT10.2724795640326975No Hit
TGGTCTCCCACAAACGTCACCACGTGACTGTA10.2724795640326975No Hit
TCTCTAACTAACGAGGCACCGTGGGGTAAA10.2724795640326975No Hit
GTATCTTGGATATGTTAGCTACGCCTTGGT10.2724795640326975No Hit
AATTGATGTCACACTGTATTGAGGGGGA10.2724795640326975No Hit
TCCCCAATTGTTCCAAGATCGGTCTCAGAGAA10.2724795640326975No Hit
TGGTCTCCCACAAACGTCACCACGTGAC10.2724795640326975No Hit
TAAGTTATCCAGGAACCATGTTAGGGCC10.2724795640326975No Hit
TAGACCCCGGTTAGTAGTGTACATGCCTG10.2724795640326975No Hit
TTTGAATAGCAGCCGTCCTTTCTGCAGG10.2724795640326975No Hit
TACATTCCCCTTTTTCATCGCTGTATATCC10.2724795640326975No Hit
TTGGATATGTTAGCTACGCCTTGGTACAG10.2724795640326975No Hit
TGTTCCAAGATCGGTCTCAGAGAATGAGGAT10.2724795640326975No Hit
TGCGATCTGTCACCTTGGAGCTTAATCCAGA10.2724795640326975No Hit
TTATCTTCAGTTAAGTGACTTGGTCTCCC10.2724795640326975No Hit
TGTGCGATCTGTCACCTTGGAGCTTAATC10.2724795640326975No Hit
TTATTATTATCAGAAGGTTGGTTGGCTATC10.2724795640326975No Hit
AACTAACACTAGTAGATCCCATAGTATG10.2724795640326975No Hit
TCTGATACAAATCACCAGTTCTGTTTGCTAT10.2724795640326975No Hit
TGGGTTGCATGGATAAGGTGTTCCGTATT10.2724795640326975No Hit
TAGCACCTAGATTCCTGATTACCCACTCC10.2724795640326975No Hit
TCTGAAACTATGTCCCCATAAACCCAGGAA10.2724795640326975No Hit
CCCCAATTGTTCCAAGATCGGTCTCAGAG10.2724795640326975No Hit
TACCCAATACCGATCGGAGATACGAATT10.2724795640326975No Hit
TTAACCCATTGTAAGCCGGCACCGCATGA10.2724795640326975No Hit
TGGTCTCCCACAAACGTCACCACGTGACTA10.2724795640326975No Hit
AACCATGTTAGGGCCAAATCTGATTCGGT10.2724795640326975No Hit
ATTGAATAAATTAGCATGGCTCACGAACG10.2724795640326975No Hit
TTGGATATGTTAGCTACGCCTTGGTACAAA10.2724795640326975No Hit
CAGGAACCATGTTAGGGCCAAAGCT10.2724795640326975No Hit
TCAGAGAATGAGGACGTCGAGTTAAC10.2724795640326975No Hit
TGATTACCCACTCCCACCATCCGTACAACT10.2724795640326975No Hit
TCCCCAATTGTTCCAAGATCGGTCTCAGAGA10.2724795640326975No Hit
TGAGGACGTCGAGTTAACTTTAATGTTGGTA10.2724795640326975No Hit
GTTCCAAGATCGGTCTCAGAGAATGAGGAC10.2724795640326975No Hit
TGAGGACGTCGAGTTAACTTTAATGTTGG10.2724795640326975No Hit
TAGCATCTACTCCTGAGCACTCTTTGTCCA10.2724795640326975No Hit
CCCAATTGTTCCAAGATCGGTCTCAGA10.2724795640326975No Hit
GAATAACAGCCGTCCTTTCTGCAGGA10.2724795640326975No Hit
CTTGGTCTCCCACAAACGTCACCACGTGAC10.2724795640326975No Hit
TGAGGACGTGGAGTTAACTTTAATGTTGGT10.2724795640326975No Hit
TATATTGTGCGATCTGTCACCTTGGAGCTT10.2724795640326975No Hit
TTTGAATAACAGCCGTCCTTTCTGCAGG10.2724795640326975No Hit
TGTCCCCAATTGTTCCAAGATCGGTCTCAGCA10.2724795640326975No Hit
ACGTCGAGTTAACTTTAATGTTGG10.2724795640326975No Hit
TTGGTCTCCCACAAACGTCACCAAGTGACTG10.2724795640326975No Hit
CCGGCACCGCATGAGAAAACGCCCCACAA10.2724795640326975No Hit
TGTGCTTTGCGATAGTAGACCATAAACCT10.2724795640326975No Hit
TTGGTAACAACTAGACCCCGGTTAGTAGT10.2724795640326975No Hit
TCTGAAACTATGTCCCCATAAACCCAGGA10.2724795640326975No Hit
AACACTAGTAGATCCCATAGTATGGCTAAAC10.2724795640326975No Hit
AATAACGATATCGGGTACTTTCCACAAGCATG10.2724795640326975No Hit
TTTATCTTCAGTTAAGTGACTTGGTCTCCC10.2724795640326975No Hit
TGTTCCAAGATCGGTCTCAGAGAATGAGGACA10.2724795640326975No Hit
TTTGTCCCCAATTGTTCCAAGATCGGTCTA10.2724795640326975No Hit
TTAAGTTATCCAGGAACCATGTTAGGGCC10.2724795640326975No Hit
TCTTGGATATGTTAGCTACGCCTTGGTA10.2724795640326975No Hit
CCCCATAAACCCAGGAGTCGTCAAAGTC10.2724795640326975No Hit
TCTGAAACTATGTCCCCATAAACCCAGGAGTCGTC10.2724795640326975No Hit
TTGGTTGGCTATCTTCTTTTCCTGAGCTCG10.2724795640326975No Hit
TACAATAACGATATCGGGTACTTTCCACA10.2724795640326975No Hit
TGTATACCGATTGTGCCATCAAAAAGAG10.2724795640326975No Hit
TCCTGAGCACTCTTTGTCCCCAATT10.2724795640326975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)