FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orbivirus.NODE_20583.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orbivirus.NODE_20583.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length15-30
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCGTTTCATCCCCTCGACTATCGACT213.333333333333334No Hit
ATCCGTTTCATCCCCTCGACTATCGACTA213.333333333333334No Hit
CCAACTTTGACCCTTGTTCCGACCGCCT213.333333333333334No Hit
CACAGATGCATCGGCATGTGGCGCTCGA213.333333333333334No Hit
GTATAGGGGAAGGAG213.333333333333334No Hit
ACCGCCGGTTCGGGC213.333333333333334No Hit
CACAGATGCATCGGCATGTGGCGCT16.666666666666667No Hit
TTTCATCCCCTCGACTATCGACTAACT16.666666666666667No Hit
TGAGTCCTCATCCGTTTCATCCCCTCGACT16.666666666666667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)