FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orbivirus.NODE_5225.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orbivirus.NODE_5225.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194
Sequences flagged as poor quality0
Sequence length15-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGAAGTTGTCCATCTTAGCGTGCTGGT852.6612398246712585No Hit
TAGTGCAAACGGCGCGGATCAGATCAACTC852.6612398246712585No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTAC692.1603005635566688No Hit
TCAACTCCGTCGACGGAAACGATTTCCCCT621.9411396368190357No Hit
TCAACTCCGTCGACGGAAACGATTTCCCC611.909830932999374No Hit
TCAAAGTTGGCGTGGCGATGGGTCCAGGT601.878522229179712No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGGC571.7845961177207263No Hit
TCGACAGGCAGGACAGAACCAGAGAGTCTCC491.5341264871634315No Hit
TCACGAGGAGGTAAAAATGGAAGGATAGG441.3775829680651221No Hit
TACAATATGTTCTCCACCCAAGCCAAGCCT371.158422041327489No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAAA361.127113337507827No Hit
TCCAGGTCTCATCGGCAAGACAACCAGCC351.0958046336881653No Hit
TCAACCAGCTCGGAGAACGAGATACCGTCAGGC331.0331872260488417No Hit
TTCTCGAGATCGTAGGCAAAGTCCCCGGCC270.8453350031308704No Hit
TTCTCCACCCAAGCCAAGCCTGAGATCGAAC270.8453350031308704No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGAA260.8140262993112084No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCAC260.8140262993112084No Hit
TGAGGGACTCACGCTCAGCACGAGAAA260.8140262993112084No Hit
TCTCGAGATCGTAGGCAAAGTCCCCGGCC250.7827175954915466No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTAC250.7827175954915466No Hit
TGAACAAGGTCCAATGAGGAGACAGCTCTCGC240.7514088916718847No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGA210.6574827802128992No Hit
TATGAAAAGGAGAAGTAGGATCAACGGGT200.6261740763932373No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGA200.6261740763932373No Hit
TGCAAACGGCGCGGATCAGATCAACTC190.5948653725735754No Hit
TACGTCCATTATCCCACCAAGTGCCATAGAC190.5948653725735754No Hit
TGAGGGACTCACGCTCAGCACGAGAA180.5635566687539135No Hit
GGCTGGGTGTCGACAGGCAGGACAGAACC170.5322479649342517No Hit
TAGGGGAGTAGCTCCGTGGTCTGGTGAGG170.5322479649342517No Hit
TACAGAGTCGATTTGAAGCAGTTTGCCCAC160.5009392611145899No Hit
TTATCCATTATTCTGACCCCCGAGGTAG150.469630557294928No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGGCG150.469630557294928No Hit
TAAGGCTCGTCGTGCCAAACGTGAGGGAC150.469630557294928No Hit
TCAACGGGTATGGGTTCAAGGAATTGAGT150.469630557294928No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTA150.469630557294928No Hit
AAGGACATCCACTTAAACCAGTTGATAAGGC150.469630557294928No Hit
TCAACTTCTCGAGATCGTAGGCAAAGTCCCC140.4383218534752661No Hit
TAAACCAGTTGATAAGGCTCGTCGTGCCAAAC140.4383218534752661No Hit
AGGAGAAGTAGGATCAACGGGTATGGGT130.4070131496556042No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACACA130.4070131496556042No Hit
TACAGAGTCGATTTGAAGCAGTTTGCCCACT130.4070131496556042No Hit
TGGGAAGGCAATCGGAGAAGGAGGACATC130.4070131496556042No Hit
TCGACAGGCAGGACAGAACCAGAGAGTCTC130.4070131496556042No Hit
CCTGAGCCTGAGCCGGAGATCCAACTT120.37570444583594237No Hit
TCACGAGGAGGTAAAAATGGAAGGATAGGA120.37570444583594237No Hit
TCGGAAGTTGTCCATCTTAGCGTGCTGGTC120.37570444583594237No Hit
TGCATCTGTGAACCCAGGATTCGGGGGT110.34439574201628054No Hit
TGAGGGACTCACGCTCAGCACGAGAAAG110.34439574201628054No Hit
TGAAGGTGGAATGAAGACTGGGAAGAGGC110.34439574201628054No Hit
TACCCGGGTACTGCACAACACTGTCCAACAC110.34439574201628054No Hit
CCTGAGCCTGAGCCGGAGATCCAACTTACT110.34439574201628054No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACAC110.34439574201628054No Hit
GCCAAGCCTGAGATCGAACACATTGAGAA100.31308703819661865No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTTA100.31308703819661865No Hit
TCCGATTAGCGCGCCCGTACCCAGCAACTT100.31308703819661865No Hit
TGCCCACTGTGCTTATGTTATCAAGGCGTG100.31308703819661865No Hit
TACAATATGTTCTCCACCCAAGCCAAGCC90.28177833437695676No Hit
TTATCCATTATTCTGACCCCCGAGGTAGA90.28177833437695676No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGAT90.28177833437695676No Hit
TACAAGGAAAAGTTGTTGAGGGCTGGTG90.28177833437695676No Hit
TCTCCACCCAAGCCAAGCCTGAGATCGAAC90.28177833437695676No Hit
TCAGTAAGGGGTACAGGGACGTCAGGGG90.28177833437695676No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGG90.28177833437695676No Hit
TACCCGGGTACTGCACAACACTGTCCAACA90.28177833437695676No Hit
TGTACAGTACAAGGAAAAGTTGTTGAGGGC90.28177833437695676No Hit
TCACGAGGAGGTAAAAATGGAAGGATAG90.28177833437695676No Hit
TGCATCTGGGAACCCAGGATTCGGGGG80.25046963055729493No Hit
TCGCTTAATCCACGCGCCATGCTATCGCCT80.25046963055729493No Hit
TCCTGAGCCTGAGCCGGAGATCCAACTT80.25046963055729493No Hit
TATGAAAAGGAGAAGTAGGATCAACGGG80.25046963055729493No Hit
TATGTTCTCCACCCAAGCCAAGCCTGAGA80.25046963055729493No Hit
TAACTGAGACCTGGACGGTCACGGCCTTGAC80.25046963055729493No Hit
TCGTCCTGAGCCTGAGCCGGAGATCCAACT80.25046963055729493No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCCAAA80.25046963055729493No Hit
TTAGCGCGCCCGTACCCAGCAACTTTCATG80.25046963055729493No Hit
TACAAGGAAAAGTTGTTGAGAGCTGGTG80.25046963055729493No Hit
TATGTACCCGGGTACTGCACAACACTGTCC80.25046963055729493No Hit
TGAGCCGGAGATCCAACTTACTAGGTGG80.25046963055729493No Hit
TCAACTCCGTCGACGGAAACGATTTCCCCTT80.25046963055729493No Hit
TCAGTAAGGGGTACAGGGACGTCAGGGGA80.25046963055729493No Hit
TGCATCTGTGAACCCAGGATTCGGGGG80.25046963055729493No Hit
TTACAGAGTCGATTTGAAGCAGTTTGCCC80.25046963055729493No Hit
TCCCCGGATATCTTTGACGAAAGACTTGG80.25046963055729493No Hit
TATCCCCGGATATCTTTGACGAAAGACTT80.25046963055729493No Hit
TACAAGGAAAAGTTGTGGAGAGCTGGTG80.25046963055729493No Hit
CGACAGGCAGGACAGAACCAGAGAGTCTCC80.25046963055729493No Hit
TCCAGGTCTCATCGGCAAGACAACCAGC80.25046963055729493No Hit
TTCTCAGTAAGGGGTACAGGGACGTCAGG70.21916092673763304No Hit
TCGAGCGCCACATGCCGATGCATCTGGGAA70.21916092673763304No Hit
TATTCTGCAGACGTCGGCCCATGTGGCGG70.21916092673763304No Hit
TCAGATCAACTCCGTCGACGGAAAGGAT70.21916092673763304No Hit
TCGGAACAAGGGTCAAAGTTGGCGTGG70.21916092673763304No Hit
TCGAGCGCCACATGCCGATGCATCTGGGA70.21916092673763304No Hit
AAGGAGAAGTAGGATCAACGGGTATGGG70.21916092673763304No Hit
TATGAGAAACTTTATGCATGGCGCGCAT70.21916092673763304No Hit
TTATCCATTATTCTGACCCCCGAGGTAGAA70.21916092673763304No Hit
TCAAAGTTGGCGTGGCGATGGGTCC70.21916092673763304No Hit
TCTCGAGATCGTAGGCAAAGTCCCCGGC70.21916092673763304No Hit
TGAAGACTGGGAAGAGGCTGGGTGTCGA60.18785222291797118No Hit
TCCCACCAAGTGCCATAGACGAAACCCTCT60.18785222291797118No Hit
TGGAATGAAGACTGGGAAGAGGCTGGGT60.18785222291797118No Hit
TCCCTCTCTGATGTCGTCCGAAGATTGCA60.18785222291797118No Hit
TTATCCCACCAAGTGCCATAGACGAAACCCT60.18785222291797118No Hit
TCAGTAAGGGGTACAGGGACGTCAGGG60.18785222291797118No Hit
TCGACAGGCAGGACAGAACCAGAGAGTCT60.18785222291797118No Hit
TAGGATCAACGGGTATGGGTTCAAGGA60.18785222291797118No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCC60.18785222291797118No Hit
TGAAGACTGGGAAGAGGCTGGGTGTCGAC60.18785222291797118No Hit
TTCCCACTAACTGAGACCTGGACGGTCACGGCCT60.18785222291797118No Hit
TAACTGAGACCTGGACGGTCACGGCCT60.18785222291797118No Hit
TCGAGCGCCACATGCCGATGCATCTGGG60.18785222291797118No Hit
TGGGAAGAGGCTGGGTGTCGACAGGCAGG60.18785222291797118No Hit
TGAAGACTGGGAAGAGGCTGGGTGTCG60.18785222291797118No Hit
TATCCCACCAAGTGCCATAGACGAAACCCT60.18785222291797118No Hit
CTTTACAATATGTTCTCCACCCAAGCCAAG60.18785222291797118No Hit
TTAAACCAGTTGATAAGGCTCGTCGTGCCAAAC60.18785222291797118No Hit
CCCGGGTACTGCACAACACTGTCCAACACC60.18785222291797118No Hit
TAGGGCTTCCAATGAAGGACATCCACTT60.18785222291797118No Hit
TGTGGCGGCGCTAGCACTAGTGCAAACGGC50.15654351909830932No Hit
TCCCACCAAGTGCCATAGACGAAACCCTC50.15654351909830932No Hit
TCTCGCTTAATCCACGCGCCATGCTATCG50.15654351909830932No Hit
TAGACGAAACCCTCTCCAAAGCCAAAATC50.15654351909830932No Hit
TCTGATGTCGTCCGAAGATTGCAATGAAG50.15654351909830932No Hit
TGACTCCACTTGGTTGATGAGCTCCTGGT50.15654351909830932No Hit
TCACGGCCTTGACACCCGAACTAGTCGAGC50.15654351909830932No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCA50.15654351909830932No Hit
TCTTGAAAAGAACTTACTCCCATGGTACG50.15654351909830932No Hit
TATGTCGTCCTGAGCCTGAGCCGGAGAA50.15654351909830932No Hit
TAGGTGGGAGCTAACTGCAACTTGAGCGT50.15654351909830932No Hit
TAGGTGGGAGCTAACTGCAACTTGAGCA50.15654351909830932No Hit
TACCGTCAGGCCCATATCTGAAATCGGGT50.15654351909830932No Hit
TGGGTTCAAGGAATTGAGTATGTATGGGA50.15654351909830932No Hit
TGAAAAGAACTTACTCCCATGGTACGAACC50.15654351909830932No Hit
TCCATTATTCTGACCCCCGAGGTAGAA50.15654351909830932No Hit
GCCAAGCCGGAGATCGAACACATTGAGAA50.15654351909830932No Hit
AACCAGTTGATAAGGCTCGTCGTGCCAAA50.15654351909830932No Hit
TCGCTTAATCCACGCGCCATGCTATCGCC50.15654351909830932No Hit
TAGGATCAACGGGTATGGGTTCAAGGAA50.15654351909830932No Hit
TCGACAGGCAGGACAGAACCAGAGAGTCTCA50.15654351909830932No Hit
AAAGGAGAAGTAGGATCAACGGGTATGG50.15654351909830932No Hit
TTATGTCGTCCTGAGCCTGAGCCGGAGA50.15654351909830932No Hit
TAGACTCTCGCTTAATCCACGCGCCATGC50.15654351909830932No Hit
TGAGGGACTCACGCTCAGCACGAGAAAGAAA50.15654351909830932No Hit
GACACAATGTCGTCGAGCACATCATAAAGG50.15654351909830932No Hit
TCGGAGAACGAGATACCGTCAGGCCCATT40.12523481527864747No Hit
TCGGAGAACGAGATACCGTCAGGCCCATA40.12523481527864747No Hit
TAGGGGAGTAGCTCCGTGGTCTGGTGAG40.12523481527864747No Hit
TTCGCAAGAGCAAGCTCCCTCTCCAGTGAC40.12523481527864747No Hit
TCCATACCAAGCTTATGTCGTCCTGAGCCT40.12523481527864747No Hit
TACAGTACAAGGAAAAGTTGTTGAGGGC40.12523481527864747No Hit
TTTGACGAAAGACTTGGGAGAGCACGACCC40.12523481527864747No Hit
TAAGGGGTACAGGGACGTCAGGGGAGA40.12523481527864747No Hit
AGGACAGAACCAGAGAGTCTCCTCTTCGC40.12523481527864747No Hit
TCAACGGGTATGGGTTCAAGGAATTGAG40.12523481527864747No Hit
TCGGAGAACGAGATACCGTCAGGCCCATATC40.12523481527864747No Hit
AACCAGTTGATAAGGCTCGTCGTGCCAAAC40.12523481527864747No Hit
CCAGGATTCGGGGGTGGTAAGCGAGAA40.12523481527864747No Hit
TACCGTCAGGCCCATATCTGAAATCGG40.12523481527864747No Hit
TAGGCGGTCGGAACAAGGGTCAAAGTTGGCT40.12523481527864747No Hit
CACATAGACTCTCGCTTAATCCACGCGCC40.12523481527864747No Hit
AAGACTGGGAAGAGGCTGGGTGTCGACA40.12523481527864747No Hit
TACAGTACAAGGAAAAGTTGTTGAGAGCT40.12523481527864747No Hit
TTTACAGAGTCGATTTGAAGCAGTTTGCCCACT40.12523481527864747No Hit
TGTATGGGAAGGCAATCGGAGAAGGAGGA40.12523481527864747No Hit
AATTATCCATTATTCTGACCCCCGAGGT40.12523481527864747No Hit
TAGGCGGTCGGAACAAGGGTCAAAGT40.12523481527864747No Hit
TGAGATCGAACACATTGAGAAGGCATTCAA40.12523481527864747No Hit
TTTGCCCACTGTGCTTATGTTATCAAGGCGTG40.12523481527864747No Hit
TGCCCACTGTGCTCATGTTATCAAGGCGTG40.12523481527864747No Hit
GAAGTCCCTCTCTGATGTCGTCCGAAGATT40.12523481527864747No Hit
TGGGGGTTGAACAAGGTCCAATGAGGAGA40.12523481527864747No Hit
TCAACCAGCTCGGAGAACGAGATACCGTC40.12523481527864747No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCACG40.12523481527864747No Hit
TCGAGATCGTAGGCAAAGTCCCCGGCCACA40.12523481527864747No Hit
TGCACAACACTGTCCAACACCATTCTTAGAA40.12523481527864747No Hit
TAGGGGAGTAGCTCCGTGGTCTGGTGA40.12523481527864747No Hit
TCTCGCAGAGTCAGTGAAGGTGGAA40.12523481527864747No Hit
TCCACCCAAGCCAAGCCTGAGATCGAACACATT40.12523481527864747No Hit
CACGCCCGTGGCTTCGTCAAACTTCTTAGG40.12523481527864747No Hit
TAGGAACCGGTGGAGATGTAGCTAGCGTC40.12523481527864747No Hit
TAGGGGAGTAGCTCCGTGGTCTGGTG40.12523481527864747No Hit
TCAACTTCTCGAGATCGTAGGCAAAGTCCC40.12523481527864747No Hit
TTACAATATGTTCTCCACCCAAGCCAAGCCT40.12523481527864747No Hit
TGAGAACCTAGGCGGTCGGAACAAGGGTC40.12523481527864747No Hit
TTCTCGAGATCGTAGGCAAAGTCCCCGGC40.12523481527864747No Hit
TGTACCCGGGTACTGCACAACACTGTCCAAC40.12523481527864747No Hit
TCTCACACAAGAGTCCAGCTCACACAAGAG40.12523481527864747No Hit
TCCAGCTCACACAAGAGTCACGAGGAGG40.12523481527864747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)