FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orbivirus.NODE_9699.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orbivirus.NODE_9699.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length17-33
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGAGAGAGAATTCGGAGCCCATCC1611.678832116788321No Hit
TAAGAAGGCGTATTGCTTCGACAGCACAG128.75912408759124No Hit
TCAGAAGAGAGAGAATTCGGAGCCCATC118.02919708029197No Hit
TCAGAAGAGAGAAAATTCGGAGCCCATC107.2992700729927No Hit
TGACTAAAATCACCCATTTACACTGCTAGAC75.109489051094891No Hit
TCAGAAGAGAGAAAATTCGGAGCCCATCC53.64963503649635No Hit
TACACGTGGCACCATTGATTCTGAGTTAGCC53.64963503649635No Hit
TAAAATCACCCATTTACACTGCTAGACGC53.64963503649635No Hit
AAATCACCCATTTACACTGCTAGACGCAGG42.9197080291970803No Hit
TCGACAGCACAGAGCTTGACTAAAATCACCC42.9197080291970803No Hit
TACACGTGGCACAATTGATTCTGAGTTAGCC42.9197080291970803No Hit
TCCAGACCTACGCCCGATTCGGATTGTTC42.9197080291970803No Hit
TCAGGAAAAAGATAGAACCGAACAGGTTTC32.18978102189781No Hit
TTGACTAAAATCACCCATTTACACTGCTG21.4598540145985401No Hit
GACTAAAATCACCCATTTACACTGCTAGAC21.4598540145985401No Hit
CTTGACTAAAATCACCCATTTACACTGCTG21.4598540145985401No Hit
TTTTCAGATGAACTAATGAACTCCGGTTTC21.4598540145985401No Hit
TTTCAGAAGAGAGAAAATTCGGAGCCCATC21.4598540145985401No Hit
TCAGAAGAGAGAAAATTCGGAGCCC21.4598540145985401No Hit
TAATGAATTCAAATTGGATCGGTGGTGCC21.4598540145985401No Hit
TGACTAAAATCACCCATTTACACTGCTGGACGC21.4598540145985401No Hit
TTCAGAAGAGAGAAAATTCGGAGCCCATC21.4598540145985401No Hit
GGCTTACACAATGCTACGCACACCGCTC21.4598540145985401No Hit
TCTGAGTTAGCCAATTCCAAGATACACTTT21.4598540145985401No Hit
TCAGAAGAGAGAGAATTCGGAGCCCA21.4598540145985401No Hit
TGACTAAAATCACCCATTTACACTGCTAG10.7299270072992701No Hit
CTAAAATCACCCATTTACACTGCTAGACGC10.7299270072992701No Hit
TAAGAAGGCGTATTGCTTCGACAGCACA10.7299270072992701No Hit
TTCGACAGCACAGAGCTTGACTAAAATCACC10.7299270072992701No Hit
TCGACAGCACAGAGCTTGACTAAAATCACCCA10.7299270072992701No Hit
CCCGATCCAACTCACATG10.7299270072992701No Hit
TATCGACGTCGCTACTTTCCACCTTAAAGG10.7299270072992701No Hit
TCCAACTCACATGGCCCCGACACA10.7299270072992701No Hit
CCGATCCAACTCACATG10.7299270072992701No Hit
TTCGACAGCACAGAGCTTGACTAAAATCACCC10.7299270072992701No Hit
TCGACAGCACAGAGCTTGACTAAAATCACC10.7299270072992701No Hit
TCAGATGAACTAATGAACTCCGGTTTCAG10.7299270072992701No Hit
TTTCAGAAGAGAGAGAATTCGGAGCCCAT10.7299270072992701No Hit
TGCCAACCCGCTCCAACTCACATGGCCCC10.7299270072992701No Hit
TCACCCATTTACACTGCTAGACGCAGGAAAAA10.7299270072992701No Hit
CCAACTCACATGGCCCCGACAAAA10.7299270072992701No Hit
TTCAGAAGAGAGAGAATTCGGAGCCCATC10.7299270072992701No Hit
TTAAGAAGGCGTATTGCTTCGACAGCACAG10.7299270072992701No Hit
TCTGAGTTAGCCAATTCCAAGATACACTT10.7299270072992701No Hit
TGAACTCCGGTTTCAGGAAAAAGATAGAAC10.7299270072992701No Hit
CCCGATCCAACTCACATGGCCCCGACACAT10.7299270072992701No Hit
GTAGAACCGAACAGGTT10.7299270072992701No Hit
TGCTTCGACAGCACAGAGCTTGACTAAAATC10.7299270072992701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)