FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orbivirus.NODE_9699.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orbivirus.NODE_9699.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962
Sequences flagged as poor quality0
Sequence length15-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGACACCGCTCAAGGATGAAAGATCATC21711.060142711518859No Hit
TTAGAGAAGAGACACCCTTCAGGTACTGT1055.351681957186544No Hit
TACACCAACCAAGTCGCCAAACTCGATAGCCCT683.4658511722731906No Hit
TCCTTGGGTATGAACAATCCGAATCGGGC572.90519877675841No Hit
TCCACTTAGAATCCTCTCTAGACCGCACCT482.4464831804281344No Hit
TAGCCCTTACAGAGACCTCACACGGAAGAG462.344546381243629No Hit
TCAATAGATCCACTTCCAGACCTCGCATC442.2426095820591234No Hit
TAAGCTGTCGCAGACGTTCAATAGATCCAC391.9877675840978593No Hit
TGAACAAGGCCCAAACGCAAAGCATAAGTG381.9367991845056065No Hit
TCCACTTAGAATCCTCTCTAGACCGCACC261.325178389398573No Hit
TATGAACAATCCGAATCGGGCGTAGGTC251.27420998980632No Hit
TTCTTGTTCAGAATCTTAACTATGGCGCC251.27420998980632No Hit
TAAGCACATAACCAAAGATTGGAGGGATG241.2232415902140672No Hit
TAACGGCGGAATCGATGGTGACTCTCCTGC221.1213047910295617No Hit
TGAGACACCCTACGGATAGGACACCGCTC190.9683995922528033No Hit
TTCAATAGATCCACTTCCAGACCTCGCATC180.9174311926605505No Hit
TCAAGCTCCGTGCTGTCGAAGCAATACGCCT180.9174311926605505No Hit
TACAAAATCCGAGGCGTAGGTGCGAGAGG180.9174311926605505No Hit
TAGGACACCGCTCAAGGATGAAAGATCA160.8154943934760449No Hit
TCAACGTCGTCTCCAACTACGCATAGGGA150.764525993883792No Hit
TTAGAATCCTCTCTAGACCGCACCTCCTT150.764525993883792No Hit
TGCTTTGGCGGAGGACCCTTGAGTACCT140.7135575942915392No Hit
TAAGCCAAATCTCTCGAACGATCTCACGAT130.6625891946992865No Hit
TCAGAAAGACCAACTCCAACACCAGTGTT130.6625891946992865No Hit
TAGAATCCTCTCTAGACCGCACCTCCTT120.6116207951070336No Hit
TAACTCCTGAGAGAACTCAAGAAAGTGGG120.6116207951070336No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCAG120.6116207951070336No Hit
TCTGATCCTTGGGTATGAACAATCCGAAT120.6116207951070336No Hit
ATCCACTTAGAATCCTCTCTAGACCGCACC110.5606523955147809No Hit
TCTGGACATATTCAGCTGAAAGAGGGCATA110.5606523955147809No Hit
TACAAAATCCGAGGCGTAGGTGCGAGAG100.509683995922528No Hit
TTGAGACACCCTACGGATAGGACACCGCTC100.509683995922528No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGGA100.509683995922528No Hit
CACCAACCAAGTCGCCAAACTCGATAGCCCT100.509683995922528No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTTCT100.509683995922528No Hit
TGAAACATAACCAATCGGGTCGCGAATCGT100.509683995922528No Hit
TAAGACGGTAGCCTCGGAATGGAGTTCGT90.45871559633027525No Hit
TTAACTCCTGAGAGAACTCAAGAAAGTGGG90.45871559633027525No Hit
TCTTGTTCAGAATCTTAACTATGGCGCCT80.40774719673802245No Hit
ACCAACCAAGTCGCCAAACTCGATAGCCCT80.40774719673802245No Hit
TGAACAATCCGAATCGGGCGTAGGTCTGGAC80.40774719673802245No Hit
TTTGGCGGAGGACCCTTGAGTACCTTGGGG70.3567787971457696No Hit
TGCGCATTTCAGCGGCGAGAGGTCCCATT70.3567787971457696No Hit
AGAGTGAGACCTAGACGGGAACCGGGAT70.3567787971457696No Hit
TAGCCCTTACAGAGACCTCACACGGAAGA70.3567787971457696No Hit
TAGAGAAGAGACACCCTTCAGGTACTGT70.3567787971457696No Hit
TAGTAGCTCCTCCTGTGCGCGACCTAGG60.3058103975535168No Hit
TCCACTTAGAATCCTCTCTAGACCGCACCTC60.3058103975535168No Hit
TAGGAGGAAATATATCGGTGAAGGCGAGG60.3058103975535168No Hit
TGTAAGACGGTAGCCTCGGAATGGAGTTC60.3058103975535168No Hit
TTCAGCTGAAAGAGGGCATAGAAACGTCT60.3058103975535168No Hit
TCAGCTGAAAGAGGGCATAGAAACGTCT60.3058103975535168No Hit
TATTCAGCTGAAAGAGGGCATAGAAACGTC60.3058103975535168No Hit
TCCCCCCCAGTAATTCGGAGTTCCCTCAGCT60.3058103975535168No Hit
CTGGACATATTCAGCTGAAAGAGGGCATA60.3058103975535168No Hit
TTCTTGTTCAGAATCTTAACTATGGCGCCT60.3058103975535168No Hit
TCGGGCGTAGGTCTGGACATATTCAGCT60.3058103975535168No Hit
TACGCATAGGGAGACGGGGGTGAGACATT60.3058103975535168No Hit
TCGACGGAGCAGAGGTCGCGTAGTAGCTC60.3058103975535168No Hit
TAGTAGCTCCTCCTGTGCGCGACCTAGGG60.3058103975535168No Hit
TACACCAACCAAGTCGCCAAACTCGATAGCCC50.254841997961264No Hit
TGTCGGGGCCATGTGAGTTGGATCGGGT50.254841997961264No Hit
TACGCATAGGGAGACGGGGGTGAGACATTT50.254841997961264No Hit
TATATCGGTGAAGGCGAGGGAGGGCTTAG50.254841997961264No Hit
TCCTCCTGTGCGCGACCTAGG50.254841997961264No Hit
TCTTGTTCAGAATCTTAACTATGGCGCCTC50.254841997961264No Hit
TCGGTGAAGGCGAGGGAGGGCTTAGTTT50.254841997961264No Hit
TCGGGCGTAGGTCTGGACATATTCAGCTG50.254841997961264No Hit
AAGGACGATTTCTCTCCACCCAGGA50.254841997961264No Hit
TACAAAATCCGAGGCGTAGGTGCGAGA50.254841997961264No Hit
AAGAGTGAGACCTAGACGGGAACCGGGA50.254841997961264No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTTC50.254841997961264No Hit
TTGGCGGAGGACCCTTGAGTACCTTGG40.20387359836901123No Hit
AGAGACACCCTTCAGGTACTGTAAGACGGT40.20387359836901123No Hit
AGAGTGAGACCTAGACGGGAACCGGGA40.20387359836901123No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCAGA40.20387359836901123No Hit
TTCGAGAGCATGAGCCTCCTACGGGA40.20387359836901123No Hit
TAAGCGATGAGTCGACGGAGCAGAGGT40.20387359836901123No Hit
TCTGAGAGCGAACCATGCGTCGCAGGGGG40.20387359836901123No Hit
TAGGACACCGCTCAAGGATGAAAGATC40.20387359836901123No Hit
TCGTCGTAACCACGGGACATCTTCGAGAG40.20387359836901123No Hit
TTGGACTCTTTGGTATATCACTAGCAAGA40.20387359836901123No Hit
TCAACACGGGATCTCTACTGATAAGCTGTC40.20387359836901123No Hit
TACACCAACCAAGTCGCCAAACTCGATAGCC40.20387359836901123No Hit
TGAACAAGGCCCAAACGCAAAGCATAAGTGAGC40.20387359836901123No Hit
ATGGGCTCCGAATTTTC40.20387359836901123No Hit
TCACGATCTCTTCGGGAGTAATATGGCCC40.20387359836901123No Hit
TGTAAGCACATAACCAAAGATTGGAGGGAT40.20387359836901123No Hit
TAGCAGTTCATTATACTTGTCGGGGCC40.20387359836901123No Hit
TCATTATACTTGTCGGGGCCATGTGAGT40.20387359836901123No Hit
TCGGAATGGAGTTCGTGGGAGGAGATAGA40.20387359836901123No Hit
TGAGACCTAGACGGGAACCGGGATAAGAA40.20387359836901123No Hit
TGAGACCTAGACGGGAACCGGGATAAGAAA40.20387359836901123No Hit
TCCTGAGAGAACTCAAGAAAGTGGGAGACT40.20387359836901123No Hit
TTCATTATACTTGTCGGGGCCATGTGAGT40.20387359836901123No Hit
GAGAAGAGACACCCTTCAGGTACTGTAAGA40.20387359836901123No Hit
TGTGTAAGCCAAATCTCTCGAACGATCTC40.20387359836901123No Hit
TCAACGTCGTCTCCAACTACGCATAGGG40.20387359836901123No Hit
TCTGGACATATTCAGCTGAAAGAGGGCACC40.20387359836901123No Hit
TATATCGGTGAAGGCGAGGGAGGGCTT30.1529051987767584No Hit
TAACCAATCGGGTCGCGAATCGTGCGAGGC30.1529051987767584No Hit
TACAAAATCCGAGGCGTAGGTGCGAGAA30.1529051987767584No Hit
TAGGGAGACGGGGGTGAGACATTTACCAAG30.1529051987767584No Hit
CACGGGACATCTTCGAGAGCATGAGCCTCCAAA30.1529051987767584No Hit
TAGGGGAAGGTTGCTTTGGCGGAGGACCC30.1529051987767584No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCAGAG30.1529051987767584No Hit
TATGAACAATCCGAATCGGGCGTAGGTCTGGAC30.1529051987767584No Hit
AATAGCAGTTCATTATACTTGTCGGGGCC30.1529051987767584No Hit
TATGAACAATCCGAATCGGGCGTAGGTCT30.1529051987767584No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCT30.1529051987767584No Hit
CACTAAAGAAAAGTGT30.1529051987767584No Hit
TAGGACACCGCTCAAGGATGAAAGATCATCA30.1529051987767584No Hit
TCACGATCTCTTCGGGAGTAATATGGCCCTTC30.1529051987767584No Hit
TTGGCGGAGGACCCTTGAGTACCTTG30.1529051987767584No Hit
TGCGCATTTCAGCGGCGAGAGGTCCCAT30.1529051987767584No Hit
TAGCTCCTCCTGTGCGCGACCTAGGGG30.1529051987767584No Hit
TCATCTGAGAGCGAACCATGCGTCGCAGG30.1529051987767584No Hit
TCAACATCAGAAAGACCAACTCCAACACCAG30.1529051987767584No Hit
TGAGTCGACGGAGCAGAGGTCGCGTAGT30.1529051987767584No Hit
TCTGGACATATTCAGCTGAAAGAGGGCA30.1529051987767584No Hit
AACCACGGGACATCTTCGAGAGCATGAGCCT30.1529051987767584No Hit
GATCCACTTAGAATCCTCTCTAGACCGCACC30.1529051987767584No Hit
TGGACATATTCAGCTGAAAGAGGGCATAGA30.1529051987767584No Hit
TAGGAGGAAATATATCGGTGAAGGCGAGGG30.1529051987767584No Hit
TAACCAATCGGGTCGCGAATCGTGCGAGGCT30.1529051987767584No Hit
TCTAACGGCGGAATCGATGGTGACTCTCC30.1529051987767584No Hit
TCGGGGCCATGTGAGT30.1529051987767584No Hit
TCAGCTGAAAGAGGGCATAGAAACGTCTT30.1529051987767584No Hit
TCACGATCTCTTCGGGAGTAATATGGCC30.1529051987767584No Hit
TAAGTGAGCGAAAGGACGATTTCTCTCCACC30.1529051987767584No Hit
AAGGACGATTTCTCTCCACCCAGGAGAT30.1529051987767584No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGGAT30.1529051987767584No Hit
TTACACCAACCAAGTCGCCAAACTCGATA30.1529051987767584No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGGAA30.1529051987767584No Hit
TGGGTGATTTTAGTCAAGCTCCGTGCTGTC30.1529051987767584No Hit
AACCACGGGACATCTTCGAGAGCATGAGCC30.1529051987767584No Hit
TCGGAGTTCCCTCAGCTTTGTTACACCAA30.1529051987767584No Hit
TTCTAACGGCGGAATCGATGGTGACTCTCCT30.1529051987767584No Hit
TTCTAACGGCGGAATCGATGGTGACTCTC30.1529051987767584No Hit
TCCTCTCTAGACCGCACCTCCTTGATAGC30.1529051987767584No Hit
TGTTACACCAACCAAGTCGCCAAACTCGAT30.1529051987767584No Hit
ACGGAAGAGTGAGACCTAGACGGGAACCGGGA30.1529051987767584No Hit
ATCCTTGGGTATGAACAATCCGAATCGGGC30.1529051987767584No Hit
ACGGGATGAAACATAACCAATCGGGTCGC30.1529051987767584No Hit
TCAGCTTTGTTACACCAACCAAGTCGCC30.1529051987767584No Hit
TAGCCCTTACAGAGACCTCACACGGAAG20.10193679918450561No Hit
ACAAAATCCGAGGCGTAGGTGCGAGAGGT20.10193679918450561No Hit
TCTTCGAGAGCATGAGCCTCCTACGGG20.10193679918450561No Hit
TAGGGAGACGGGGGTGAGACATTTACC20.10193679918450561No Hit
TCTCAACGTCGTCTCCAACTACGCATAGG20.10193679918450561No Hit
TAAGCGATGAGTCGACGGAGCAGAGGTCGC20.10193679918450561No Hit
GAGGGCATAGAAACGTCTTTTCTTTTGAAAGC20.10193679918450561No Hit
TAAAAACGCGATCCACTTAGAATCCTCTCT20.10193679918450561No Hit
GACGTTCAATAGATCCACTTCCAGACCTC20.10193679918450561No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTTCCT20.10193679918450561No Hit
CACGGGACATCTTCGAGAGCATGAGCCT20.10193679918450561No Hit
TAGAGAACCTGTAAGCACATAACCAAAGAT20.10193679918450561No Hit
TCCACCCAGGAGATGAAAGAAGAGCGCGTCC20.10193679918450561No Hit
TCGATGGTGACTCTCCTGCTCTGATCAAAA20.10193679918450561No Hit
TATGAACAATCCGAATCGGGCGTAGGT20.10193679918450561No Hit
TCAATAGATCCACTTCCAGACCTCGC20.10193679918450561No Hit
TCGGGTCGCGAATCGTGCGAGGCTT20.10193679918450561No Hit
TCAGCTGAAAGAGGGCATAGAAACGTCTTT20.10193679918450561No Hit
TAAGCTGTCGCAGACGTTCAATAGATCCACT20.10193679918450561No Hit
TGAACAATCCGAATCGGGCGTAGGTCTGGA20.10193679918450561No Hit
AAGGACGATTTCTCTCCACCCAG20.10193679918450561No Hit
TGGGCTCCGAATTTTCTCTCTTCTGAAAT20.10193679918450561No Hit
CACGGGACATCTTCGAGAGCATGAGCCTCC20.10193679918450561No Hit
AGAGACACCCTTCAGGTACTGTAAGACGG20.10193679918450561No Hit
CAACCAAGTCGCCAAACTCGATAGCCCT20.10193679918450561No Hit
TCCACTTCCAGACCTCGCATCATTCAAGAC20.10193679918450561No Hit
TCGGAATGGAGTTCGTGGGAGGAGATC20.10193679918450561No Hit
TAACTCAGAATCAATGGTGCCACGTGTAAA20.10193679918450561No Hit
TAATAACAATGTTAGGTAGAGCGGGAAGT20.10193679918450561No Hit
TACCTTGGGGAAAAGAACGTCTTATACTT20.10193679918450561No Hit
CGAAAGGACGATTTCTCTCCACCCAGG20.10193679918450561No Hit
TCAATAGATCCACTTCCAGACCTCGCATCA20.10193679918450561No Hit
AGCCAAATCTCTCGAACGATCTCACGA20.10193679918450561No Hit
TTAAGCGATGAGTCGACGGAGCAGAGGTC20.10193679918450561No Hit
TGTGAGTTGGATCGGGTTGGCACCAC20.10193679918450561No Hit
TTAGTCAAGCTCCGTGCTGTCGAAGCAATACGCCT20.10193679918450561No Hit
TCTGGACATATTCAGCTGAAAGAGGGC20.10193679918450561No Hit
TTGGACTCTTTGGTATATCACTAGCAAG20.10193679918450561No Hit
TCAACATCAGAAAGACCAACTCCAACACCAGT20.10193679918450561No Hit
CACACGGAAGAGTGAGACCTAGACGGGAACCGGGA20.10193679918450561No Hit
TCTGGACATATTCAGCTGAAAGAGGGCAT20.10193679918450561No Hit
TAGGACACCGCTCAAGGATGAAAGATCAT20.10193679918450561No Hit
TAACCACGGGACATCTTCGAGAGCATGAGC20.10193679918450561No Hit
TCAAGAAAGTGGGAGACTATAGAGGAA20.10193679918450561No Hit
TCCCCCCCAGTAATTCGGAGTTCCCTCAGC20.10193679918450561No Hit
TGAGACCTAGACGGGAACCGGGATAAGA20.10193679918450561No Hit
TTGGAATTGGCTAACTCAGAATCAATGGTG20.10193679918450561No Hit
GTGAGTTGGATCGGGTTGGCACCACCGAACAA20.10193679918450561No Hit
TCGGGGCCATGTGAGTTGGATCGGGT20.10193679918450561No Hit
TCTGATCCTTGGGTATGAACAATCCGAAA20.10193679918450561No Hit
TTACACCAGCGCTCTTTACGACTTAAGC20.10193679918450561No Hit
TCAAGAAAGTGGGAGACTATAGAGGAAGT20.10193679918450561No Hit
TAAAAACGCGATCCACTTAGAATCCTCTC20.10193679918450561No Hit
GAAAGATCATCAGAGAGCGAACCATGCGTC20.10193679918450561No Hit
TTTTAGTCAAGCTCCGTGCTGTCGAAGCA20.10193679918450561No Hit
TCTGGACATATTCAGCTGAAAGAGGGCATC20.10193679918450561No Hit
TCAAGAAAGTGGGAGACTATAGAGGAAG20.10193679918450561No Hit
TCCATACAAAATCCGAGGCGTAGGTGCG20.10193679918450561No Hit
ATGCGCATTTCAGCGGCGAGAGGTC20.10193679918450561No Hit
TCAACATCAGAAAGACCAACTCCAACACC20.10193679918450561No Hit
ACGGATAGGACACCGCTCAAGGATGAAAG20.10193679918450561No Hit
GTTGGATCGGGTTGGCACCA20.10193679918450561No Hit
AATGCGCATTTCAGCGGCGAGAGGTCCCAT20.10193679918450561No Hit
TTCGAGAGCATGAGCCTCCTACGGGATGA20.10193679918450561No Hit
TACGGATAGGACACCGCTCAAGGATGAAAG20.10193679918450561No Hit
TCGGTGAAGGCGAGGGAGGGCTTAGTTTC20.10193679918450561No Hit
TTGTTACACCAACCAAGTCGCCAAACTCGAT20.10193679918450561No Hit
TCAGAATCTTAACTATGGCGCCTCTAATC20.10193679918450561No Hit
TTGGGTATGAACAATCCGAATCGGGC20.10193679918450561No Hit
TTGGCGGAGGACCCTTGAGTACCTTGGGG20.10193679918450561No Hit
TTGCTTTGGCGGAGGACCCTTGAGTACCT20.10193679918450561No Hit
TAACCACGGGACATCTTCGAGAGCATGAGCC20.10193679918450561No Hit
TCTGTTCTAACGGCGGAATCGATGGTGAC20.10193679918450561No Hit
TAGCTCCTCCTGTGCGCGACCTAGGGGA20.10193679918450561No Hit
TCAAGTTCCGTGCTGTCGAAGCAATACGCCT20.10193679918450561No Hit
TCTTAGGAGGAAATATATCGGTGAAGGC20.10193679918450561No Hit
TGGGTATGAACAATCCGAATCGGGCGTAGGT20.10193679918450561No Hit
GTAACCACGGGACATCTTCGAGAGCATGAGC20.10193679918450561No Hit
TGTTCTAACGGCGGAATCGATGGTGACTC20.10193679918450561No Hit
TCTTCGAGAGCATGAGCCTCCTACGGGA20.10193679918450561No Hit
TCCGTGCTGTCGAAGCAATACGCCTTCTT20.10193679918450561No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTT20.10193679918450561No Hit
TTCTTGAGACACCCTACGGATAGGACAC20.10193679918450561No Hit
TGGGTGATTTTAGTCAAGCTCCGTGCTGT20.10193679918450561No Hit
TAGCTCCTCCTGTGCGCGACCTAGGG20.10193679918450561No Hit
ACGGCGGAATCGATGGTGACTCTCCTGCT20.10193679918450561No Hit
GATAGAGAACCTGTAAGCACATAACCAAAG20.10193679918450561No Hit
CTTGTCGGGGCCATGTGAGTTGGATCG20.10193679918450561No Hit
TACGGGATGAAACATAACCAATCGGGTCG20.10193679918450561No Hit
TGAGCCGCAAAATCGTCGTAACCACGGGAC20.10193679918450561No Hit
TCCGAATCGGGCGTAGGTCTGGACATATT20.10193679918450561No Hit
TCCGAGGCGTAGGTGCGAGAGGTACTTCAA20.10193679918450561No Hit
TGGTGACTCTCCTGCTCTGATCAAAGGAAT20.10193679918450561No Hit
TTAACTATGGCGCCTCTAATCGAAAAACGT20.10193679918450561No Hit
TAAGCCAAATCTCTCGAACGATCTCACGATC20.10193679918450561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)