FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orthomyxovirus.NODE_2459.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orthomyxovirus.NODE_2459.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5996
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGAACACCTCTGTAGATAGCAGCGAG2464.10273515677118No Hit
TTCGCTTCTGGCACAACGACACTGGCTACTG2303.835890593729153No Hit
TAGCAGCGAGACACGAACCGGCGACATGGC1662.7685123415610406No Hit
TAGCAGCGAGACACGAACCGGCGACATGG1462.4349566377585057No Hit
TCCGATTGACGGCTTCGCTTCTGGCACAAC1322.201467645096731No Hit
TTCACGAACACCTCTGTAGATAGCAGCGAG831.3842561707805203No Hit
TAAGTATGATGTATACCTTGGGTGGAGCC751.2508338892595063No Hit
TGATAAAGGACGGTAGACCGTGATGGGAA570.9506337558372248No Hit
TTCACGAACACCTCTGTAGATAGCAGCGA560.9339559706470981No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAAC540.9006004002668445No Hit
TAAACTGGCAGCATCGGTCGTACTTTGATC490.8172114743162108No Hit
TGATAAAGGACGGTAGACCGTGATGGGA480.8005336891260841No Hit
TCCTAGGCGACACCGTTTGGCAACGGGC460.7671781187458305No Hit
TTGTAAGTGCGCACTAGCTCCTCTGAAGTT460.7671781187458305No Hit
TGTCCAAGTCTTAATTCCTTGAGTCGGAGCC450.7505003335557039No Hit
TGATGAACTTTTTGATCTAAACTGGCAGC390.6504336224149433No Hit
TCACGAACACCTCTGTAGATAGCAGCGA390.6504336224149433No Hit
TCTGGGAGAGGACTCTCCGTTCGACCGCCT390.6504336224149433No Hit
TAGATAGCAGCGAGACACGAACCGGCGAC380.6337558372248165No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAACA370.6170780520346898No Hit
TCATTTCTCGAGAGCGCTTGTTTGGGGG370.6170780520346898No Hit
TCCACGAACCTCAGCTTTGCCTCTTCGGCAGT350.5837224816544363No Hit
TTCAAAATCCCTCGCACCCTGTCCTTGTC330.5503669112741828No Hit
TAAGGGCTTCCACGAACCTCAGCTTTGCCT330.5503669112741828No Hit
TCTGTCGTTTCGTATAAACATGCACCCCGG310.5170113408939293No Hit
TAGATAGCAGCGAGACACGAACCGGCG310.5170113408939293No Hit
TAGCAGCGAGACACAAACCGGCGACATGG300.5003335557038026No Hit
TCTGGCCTGTGGTTGTGGTTGTTGTTGT300.5003335557038026No Hit
CCTCCGATTGACGGCTTCGCTTCTGGCACA290.4836557705136758No Hit
TCAAACGGTTTAGTTGCAGTAGGGGTTT280.46697798532354906No Hit
TCAAAACCTCCCTCTATTGTAGATTTGAGC280.46697798532354906No Hit
TCAGTCTTACTTCGATCAACTCTAACAGGT250.4169446297531688No Hit
TCAAAATCCCTCGCACCCTGTCCTTGTCT250.4169446297531688No Hit
TACTTCGATCAACTCTGACAGGATCAGTC240.40026684456304207No Hit
TTGTAAGTGCGCACTAGCTCCTCTGAAGT230.38358905937291526No Hit
TTCCGGACACCATGAACTTCATATCCACCT230.38358905937291526No Hit
TCTTACTTCGATCAACTCTGACAGGATCAG230.38358905937291526No Hit
TCCGATTGACGGCTTCGCTTCTGGCACA220.3669112741827885No Hit
TGATGTATACCTTGGGTGGAGCCGGAGC220.3669112741827885No Hit
TAAGTGCGCACTAGCTCCTCTGAAGTTGG210.35023348899266177No Hit
TTTGACACAAGCTGTTAGCGGGCCCAGACT210.35023348899266177No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAA210.35023348899266177No Hit
TAGCAGCGAGACACGAACCGGCGACATGGCAC210.35023348899266177No Hit
TGAAGACGGTGCCCCGGAATACAGTGCGT200.333555703802535No Hit
TCTGGGAGAGGACTCTCCGTTCGACCGCC200.333555703802535No Hit
TACTTCGATCAACTCTGACAGGATCAGTCT200.333555703802535No Hit
TCATAGCCTCTCCAACCTTCACCAGGAAA200.333555703802535No Hit
TTCAAACGGTTTAGTTGCAGTAGGGGTT190.31687791861240827No Hit
TCATAGCCTCTCCAACCTTCACCAGGA190.31687791861240827No Hit
TCGGCAGTGTCTGGCCTGTGGTTGTGGTT190.31687791861240827No Hit
TTTGACTTCAATCAATGACTGCCTCCCCT180.3002001334222815No Hit
TTAGACTTGTTTCCCTCCCCACCCACTCT180.3002001334222815No Hit
TGTTTCCCTCCCCACCCACTCTTTTCCACT180.3002001334222815No Hit
TATACCTTGGGTGGAGCCGGAGCGCACTG180.3002001334222815No Hit
TGATGAACTTCTTGATCTAAACTGGCAGC180.3002001334222815No Hit
TCATAGCCTCTCCAACCTTCACCAGGAA170.2835223482321548No Hit
TCGCTTCTGGCACAACGACACTGGCTACTG170.2835223482321548No Hit
TACCTTGGGTGGAGCCGGAGCGCACTGG160.26684456304202797No Hit
TCCGATTGACGGCTTCGCTTCTGGCACAA160.26684456304202797No Hit
TGCCTCTTCGGCAGTGTCTGGCCTGTGGT160.26684456304202797No Hit
AGCGAGACACGAACCGGCGACATGGCACTC150.2501667778519013No Hit
TACGAACCTCAGCTTTGCCTCTTCGGCAGT150.2501667778519013No Hit
TGGGCCCAAATTTCTGGAGGATGTCGATCA150.2501667778519013No Hit
TAGCAGCGAGACACAAACCGGCGACATG150.2501667778519013No Hit
TAGATAGGTCCTGGGCTGATGTGAGCT150.2501667778519013No Hit
CACGAACCTCAGCTTTGCCTCTTCGGCAGT150.2501667778519013No Hit
TGTTGATAAAGGACGGTAGACCGTGATGG150.2501667778519013No Hit
TAGCAGCGAGACACGAACCGGCGACATG150.2501667778519013No Hit
TACCTTGGGTGGAGCCGGAGCGCACT150.2501667778519013No Hit
TTCGCTTCTGGCACAACGACACTGGCTACT140.23348899266177453No Hit
TAAACATGCACCCCGGTACTTTTCCTTGC140.23348899266177453No Hit
TCCAAGTCTTAATTCCTTGAGTCGGAGCC140.23348899266177453No Hit
TACGTGATGACGAGCTTCCAGGCCCAGAG140.23348899266177453No Hit
TGTAGATAGCAGCGAGACACGAACCGGCG140.23348899266177453No Hit
TGTGAGCTCGAGCTTTGTCAATCTGACAGGA140.23348899266177453No Hit
CTCCGATTGACGGCTTCGCTTCTGGCACAAC140.23348899266177453No Hit
TCCCCTAGGTCTAAGTGTTGATAGAATCT130.21681120747164775No Hit
TAGCACCCTGTCCTTGTCTTTATGTTGGC130.21681120747164775No Hit
TCTTACTTCGATCAACTCTGACAGGATCAGT130.21681120747164775No Hit
TCCACGAACCTCAGCTTTGCCTCTTCGG130.21681120747164775No Hit
TCCGGACACCATGAACTTCATATCCACCTC130.21681120747164775No Hit
TCCCCATGATTTATGGCTGACACTGTCCC130.21681120747164775No Hit
TAAGACATTCCAGTCCCTTCCACTGCCAGT130.21681120747164775No Hit
CCTCCGATTGACGGCTTCGCTTCTGGCAC130.21681120747164775No Hit
TCCACGAACCTCAGCTTTGCCTCTTCCTC130.21681120747164775No Hit
TGCACCCCGGTACTTTTCCTTGCACTGAAACAA120.20013342228152103No Hit
TACGTGATGACGAGCTTCCAGTCCCAGAG120.20013342228152103No Hit
TCTGCTTTTTGAACATACTCTCACCTTGGTC120.20013342228152103No Hit
TAGTCTGATATGTTCGATTAGTGTCGAGT120.20013342228152103No Hit
TATGATGTATACCTTGGGTGGAGCCGGAA120.20013342228152103No Hit
TGATATCGTATAGCTCACCTTTCCGCAAC120.20013342228152103No Hit
TGTATACCTTGGGTGGAGCCGGAGCGCAC120.20013342228152103No Hit
TAAACATGCACCCCGGTACTTTTCTTTGCAC110.18345563709139426No Hit
TTCGGCAGTGTCTGGCCTGTGGTTGTGGT110.18345563709139426No Hit
TGTAAGTGCGCACTAGCTCCTCTGAAGTT110.18345563709139426No Hit
TCCCTCGCACCCTGTCCTTGTCTTTATGTT110.18345563709139426No Hit
TAGACTTGTTTCCCTCCCCACCCACTCT110.18345563709139426No Hit
TGCACCCCGGTACTTTTCTTTGCACTGAAAC110.18345563709139426No Hit
TACAATATCCTAGGCGACACCGTTTGGC110.18345563709139426No Hit
TCTTCGGCAGTGTCTGGCCTGTGGTTGTG100.1667778519012675No Hit
TTCAGGGTGCTGAGATGAATTGAAATGG100.1667778519012675No Hit
TTATAAGTATGATGTATACCTTGGGTGGAG100.1667778519012675No Hit
TCTTACTTCGATCAACTCTGACGGGATCAG100.1667778519012675No Hit
TTCCACGAACCTCAGCTTTGCCTCTTCGG100.1667778519012675No Hit
TCCAAGTCTTAATTCCTTGAGTCGGAGCCC100.1667778519012675No Hit
TGTTGCAGAAAGAAACTCTGTCGCCGAACCT100.1667778519012675No Hit
TAAACTGGCAGCATCGGTCGTACTTTGATT100.1667778519012675No Hit
TCCGGACACCATGAACTTCATATCCACCTCC100.1667778519012675No Hit
TGTGAGCTCGAGCTTTGTCAATCTGACAGG100.1667778519012675No Hit
TGATCTAAACTGGCAGCATCGGTCGTACT100.1667778519012675No Hit
TTCCAGTCCCTTCCACTGCCAGTGAGTTAAC100.1667778519012675No Hit
TCAGTCTTACTTCGATCAACTCTGACGGGA100.1667778519012675No Hit
CACGAACACCTCTGTAGATAGCAGCGAG100.1667778519012675No Hit
TCCCATCTGCCAAAGTGACTGTGAAATCTT90.15010006671114076No Hit
TGATGTATACCTTGGGTGGAGCCGGA90.15010006671114076No Hit
TAGCAGCGAGACACAAACCGGCGACATGGC90.15010006671114076No Hit
TCAGAAAGAAACTCTGTCGCCGAACCTCC90.15010006671114076No Hit
AAGGGCTTCCACGAACCTCAGCTTTGCCT90.15010006671114076No Hit
TGAGCATAGATTCTGGATAGAAAGCGAGG90.15010006671114076No Hit
CGAAAGATCCTCTTCATCAGACCCTCCTC90.15010006671114076No Hit
TCATACAATATCCTAGGCGACACCGTTTGGC90.15010006671114076No Hit
TGCTCACATTCAGCCTGGTTGCCTGGCCACCC90.15010006671114076No Hit
TAAGTATGATGTATACCTTGGGTGGAGC90.15010006671114076No Hit
TGTCTTTATGTTGGCGGATCATGGGCCAA90.15010006671114076No Hit
TCCACGAACCTCAGCTTTGCCTCTTCCGGCC90.15010006671114076No Hit
TAAGTGAACGAGATAAGAGGGCTCATAAC90.15010006671114076No Hit
TCCCTTCCACTGCCAGTGAGTTAACCAGTT90.15010006671114076No Hit
TCATTTCTCGAGAGCGCTTGTTTGGGG80.13342228152101399No Hit
TGAAGACGGTGCCCCGGAAGACAGTGCGT80.13342228152101399No Hit
TTTCCGGACACCATGAACTTCATATCCACCT80.13342228152101399No Hit
ATAGCAGCGAGACACGAACCGGCGACATGG80.13342228152101399No Hit
TGACGGCTTCGCTTCTGGCACAACGACACT80.13342228152101399No Hit
AAAGATCCTCTTCATCAGACCCTCCTCGA80.13342228152101399No Hit
TCCAGTCCCAGAGTGCTCACATTCAGCCTG80.13342228152101399No Hit
TGATGTATACCTTGGGTGGAGCCGGAA80.13342228152101399No Hit
TGAACTTCATATCCACCTCCTCTCCCAACT80.13342228152101399No Hit
TCGGCAGTGTCTGGCCTGTGGTTGTGGT80.13342228152101399No Hit
TGTCCAAGTCTTAATTCCTTGAGTCGGAGC80.13342228152101399No Hit
TCTCTCGCACCCTGTCCTTGTCTTTATGT80.13342228152101399No Hit
TATGATGTATACCTTGGGTGGAGCCGGAG80.13342228152101399No Hit
TTCTCGAGAGCGCTTGTTTGGGGGCATC80.13342228152101399No Hit
TGATGAACTTCTTGATCTAAACTGGCAGGA80.13342228152101399No Hit
GCGGCGACAATGAAGACGGTGCCCCGGAA80.13342228152101399No Hit
TACTTCGATCAACTCTGACAGGATCAGT70.11674449633088727No Hit
TCATTTCTCGAGAGCGCTTGTTTGGGGGC70.11674449633088727No Hit
GCAGCGAGACACGAACCGGCGACATGGCACT70.11674449633088727No Hit
TCTTCGGCAGTGTCTGGCCTGTGGTTGT70.11674449633088727No Hit
TACTGGCAGCATCGGTCTTACTTCGATC70.11674449633088727No Hit
TACAATATCCTAGGCGACACCGTTTGGCAAC70.11674449633088727No Hit
TTTGCCTCTTCGGCAGTGTCTGGCCT70.11674449633088727No Hit
TCTGTCGTTTCGTATAAACATGCACCCCGGT70.11674449633088727No Hit
TAGTGACGAGCGTACCTGTTATAAGTATG70.11674449633088727No Hit
TTCCGGACACCATGAACTTCATATCCACC70.11674449633088727No Hit
TTTGCCTCTTCGGCAGTGTCTGGCCTGTGG70.11674449633088727No Hit
TTCGGCAGTGTCTGGCCTGTGGTTGTGG70.11674449633088727No Hit
TTTGCCTCTTCGGCAGTGTCTGGCCTG70.11674449633088727No Hit
TTGTCTTTATGTTGGCGGATCATGTGCT70.11674449633088727No Hit
TTCGACAACACCGTTCATATCCCTCTTGT70.11674449633088727No Hit
TGCTTTTTGAACATACTCTCACCTTGGTC70.11674449633088727No Hit
TGCGCACTAGCTCCTCTGAAGTTGGTT70.11674449633088727No Hit
ACCTGGGTGGAACAAGTAAACTGAAGTCT70.11674449633088727No Hit
TACCTTGGGTGGAGCCGGAGCGCACTG70.11674449633088727No Hit
TCCCTCGCACCCTGTCCTTGTCTTTATGT70.11674449633088727No Hit
TAGCAGCGAGACACGAACCGGCGACATGGCA70.11674449633088727No Hit
TCAATGGCAAGTCCGTTCTTGTGGGCATC70.11674449633088727No Hit
TCCACTTGGTATTTCCTCAGTTCATCGGCT70.11674449633088727No Hit
TCAAAATCCCTCGCACCCTGTCCTTGTCTT70.11674449633088727No Hit
TAAACATGCACCCCGGTACTTTTCCTTGCA70.11674449633088727No Hit
TTCAAAATCCCTCGCACCCTGTCCTTGTCT70.11674449633088727No Hit
TCTGCCATGGGCTGTATTCATAATGACCCG70.11674449633088727No Hit
TTTCCGGACACCATGAACTTCATATCCACC70.11674449633088727No Hit
TCTGGCCTGTGGTTGTGGTTGTTGTTGTT70.11674449633088727No Hit
TTCATTTCTCGAGAGCGCTTGTTTGGGG70.11674449633088727No Hit
TTGGCAACGGGCGCCTAGTTTAAGGGCTT70.11674449633088727No Hit
TGATCTAAACTGGCAGCATCAGTCGTACT70.11674449633088727No Hit
TCTTCGGCAGTGTCTGGCCTGTGGTTGTT70.11674449633088727No Hit
AGCGAGACACAAACCGGCGACATGGCACTC70.11674449633088727No Hit
TAGATAGGTCCTGGGCTGATGTGAGTTC70.11674449633088727No Hit
TCTCTCGCACCCTGTCCTTGTCTTTATGTT70.11674449633088727No Hit
TAAGGGCTTCGATAAACCTCTGTTGGGAC60.10006671114076052No Hit
TCGGTGTTGATAAAGGACGGTAGACCGTGAT60.10006671114076052No Hit
TAAGGGCTTCCACGAACCTCAGCTTTGCC60.10006671114076052No Hit
TGATAAAGGACGGTAGACCGTGATGG60.10006671114076052No Hit
TCCGATTGACGGCTTCGCTTCTGGCAC60.10006671114076052No Hit
TCTTACTTCGATCAACTCTGACGGGATCA60.10006671114076052No Hit
TTGGGAACGGGCGCCTAGTTTGAGGGCTT60.10006671114076052No Hit
TGCCTCCAAAACCACGACACACTCCCCATG60.10006671114076052No Hit
TGATAAAGGACGGTAGACCGTGATGGG60.10006671114076052No Hit
TACTTCGATCAACTCTGACGGGATCAGTC60.10006671114076052No Hit
TACCTTGGGTGGAGCCGGAGCGCACTGGGC60.10006671114076052No Hit
CCTCCTCGAGGTCGTCTTTTATGAACATCG60.10006671114076052No Hit
TAAACATGCACCCCGGTACTTTTCTTTGCACT60.10006671114076052No Hit
AACCTGGGTGGAACAAGTAAACTGAAGTCT60.10006671114076052No Hit
TCTGTAGATAGCAGCGAGACACGAACCGGC60.10006671114076052No Hit
TCCCTTCCACTGCCAGTGAGTTAACCAGT60.10006671114076052No Hit
TGCAGTCATTCACGACGTGCCAGTGGTC60.10006671114076052No Hit
CTCCGATTGACGGCTTCGCTTCTGGCACA60.10006671114076052No Hit
TACTAATTTAAGCGACACCGTTTGGGAAC60.10006671114076052No Hit
TCGTGTAGTACTCTGTTCCTACAAGGAGCT60.10006671114076052No Hit
TCAAACGGTTTAGTTGCAGTAGGGGTTTT60.10006671114076052No Hit
TGCACCCCGGTACTTTTCTTTGCACTGAAACA60.10006671114076052No Hit
TCATATCCACCTCCTCTCCCAACTTAGACT60.10006671114076052No Hit
TCTTACTTCGATCAACTCTGACAGGATCA60.10006671114076052No Hit
TTGGGAACGGGCGCCTAGTTTGAGGGCTTC60.10006671114076052No Hit
TATCCTAGGCGACACCGTTTGGCAACGGGC60.10006671114076052No Hit
TTTGCCTCTTCGGCAGTGTCTGGCCTGTGGT60.10006671114076052No Hit
TGCCTCTTCGGCAGTGTCTGGCCTGTGG60.10006671114076052No Hit
TCTCCGTTCGACCGCCAATCCCTTCATAGT60.10006671114076052No Hit
TGCTCACATTCAGCCTGGTTGCCTGGCCACC60.10006671114076052No Hit
TCGGTGTTGATAAAGGACGGTAGACCGTG60.10006671114076052No Hit
TTGGAGAGGATAGATAGGTCCTGGGCTGAT60.10006671114076052No Hit
TCCACGAACCTCAGCTTTGCCTCTTCCGG60.10006671114076052No Hit
TGTCCAAGTCTTAATTCCTTGAGTCGGAGCCA60.10006671114076052No Hit
TCATAGCCTCTCCAACCTTCACCAGGAAAG60.10006671114076052No Hit
TCCCCATGATTTATGGCTGACACTGTCCCATC60.10006671114076052No Hit
TCACATTCAGCCTGGTTGCCTGGCCACCC60.10006671114076052No Hit
TTCCAGTCCCTTCCACTGCCAGTGAGTT60.10006671114076052No Hit
TACTTCGATCAACTCTGACGGGATCAGTCT60.10006671114076052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)