FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orthomyxovirus.NODE_2459.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orthomyxovirus.NODE_2459.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length15-31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGGATTTTGAAG624.0No Hit
TTCATCTCAGCACCCTGAATCGAACGGAATC416.0No Hit
TGGCGGTCGAACGGAGAGTCCTCTC312.0No Hit
AATGTGAGCACTCTGGGACTGGAAGCT28.0No Hit
AACAGTAAAACCCAGACCCAATGCTCCT28.0No Hit
CCGGGGTGCATGTTTATACGAAACGACAGAC28.0No Hit
TTCTGCAACAACAACAACCACAACCACAGG14.0No Hit
CGAAGTAAGACTGATCCCGTCAGAGT14.0No Hit
AACAACAACCACAACCACAGGCCAGACACTG14.0No Hit
AGAAAGCTAATGGCACTGGCGGACT14.0No Hit
AAGACTTGGACACCACGAGTACGGGACTT14.0No Hit
ATTGAACCCATAACCTTCTCCTACTTTG14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)