FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orthomyxovirus.NODE_284.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orthomyxovirus.NODE_284.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5963
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACGAGGAACACCTTACTCAGGAGGA238640.013416065738724No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAGC2504.192520543350662No Hit
TGGCACTCTTGTTGAGATTCTCGGATGAG1081.8111688747274861No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAA1071.7943987925540836No Hit
AACCACTGGCTGCTTTCTCAAATCGTCGAG711.1906758343115882No Hit
TCAAATCGTCGAGGACGCACTAGTAAGC631.0565151769243668No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAGC611.0229750125775616No Hit
TCGACATAGCCCTTAACATTTACAACCACT611.0229750125775616No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGC590.9894348482307562No Hit
CCACTGGCTGCTTTCTCAAATCGTCGAGG560.9391246017105485No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAGG520.8720442730169378No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAGT500.8385041086701325No Hit
GAGGAACGAGGAACACCTTACTCAGGAGG470.7881938621499246No Hit
TAGCTGCTTTCTCAAATCGTCGAGGACGC470.7881938621499246No Hit
TCAAATCGTCGAGGACGCAGTAGTAAGC450.7546536978031192No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAG440.7378836156297166No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAG420.7043434512829113No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTC330.5534127117222875No Hit
TGCTTTCTCAAATCGTCGAGGACGCACT300.5031024652020796No Hit
TCTCAAATCCTCGAGGACGCACTAGTAAGC280.46956230085527423No Hit
TCCCAAATCGTCGAGGTCGTGATAGAAACC250.41925205433506624No Hit
TTCTCAAATCGCCGAGGACGCACTAGTAAGC240.4024819721616636No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAGC240.4024819721616636No Hit
AGGAACGAGGAACACCTTACTCAGGAGGA240.4024819721616636No Hit
TGTAATCATCTTCAAGTGCTGGGCGCTCC230.38571188998826095No Hit
TCCCAAATCGTCGAGGACGCACTAGAAAG230.38571188998826095No Hit
TGCTTTCTCAAATCGTCGAGGTCGTGAT220.3689418078148583No Hit
TCGTCGAGGTCGCACTAGTAAGCAATACT210.35217172564145566No Hit
TCCCAAATCGTCGAGGACGCACTAGAAAGC200.335401643468053No Hit
TAACATTTACAACCACTGGCTGCTTTCTC190.3186315612946503No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGGA190.3186315612946503No Hit
TATGTTTGCACCCAACCCGATTGATGAC190.3186315612946503No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGCA180.30186147912124767No Hit
TTCTCAAATCGTCGAGGACGCACTAGTAAGC160.26832131477444243No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAG160.26832131477444243No Hit
TCCCAAATCGTCGAGGACGCACTAGTAAGC160.26832131477444243No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAC150.2515512326010398No Hit
TAACTTTCCGCCCCGCCCTTAGCAAAAG150.2515512326010398No Hit
TCAACAGCTTTCCCAAATCGTCGAGGACGC140.23478115042763711No Hit
TGCATGCAGCCCATTTGGCCTTCCCGCCAGC140.23478115042763711No Hit
TCCGACTCTCGTCTTATTCTGTACCTTCCT140.23478115042763711No Hit
GCTGGCACTCTTGTTGAGATTCTCGGATG130.21801106825423444No Hit
TTTATCAGCGTGGTGTGTGGCGGTGCCTGC130.21801106825423444No Hit
TTTGTCAGCGTGGTGTGTGGCGGTGCCT130.21801106825423444No Hit
TGATACTCGAAGAACACTTTCTCCCTCCCT120.2012409860808318No Hit
TCAAATCGTCGAGGACGCACTAGTAAG120.2012409860808318No Hit
TGGCACTCTTGTTGAGATTCTCGGATGA120.2012409860808318No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAGG120.2012409860808318No Hit
CCACTGGCTGCTTTCCCAAATCGTCGAGG120.2012409860808318No Hit
TGAGGAGACTCTTGATGACAGATTTGCCT120.2012409860808318No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGGT120.2012409860808318No Hit
CCACTGGCTGCTTTCTCAAATCGTCGAGGT120.2012409860808318No Hit
TCAAATCGTCGAGGTCGTGATAGAAACCA120.2012409860808318No Hit
TTGGGACTTGGGCGTTAGTCTGCTTGGCT110.18447090390742915No Hit
TAAGATGACCCTCACTCTGATAGATGGAT110.18447090390742915No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAG110.18447090390742915No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAG110.18447090390742915No Hit
TACATGCAGCCCATTTGGCCTTCCCGCCAGC110.18447090390742915No Hit
TTATCAGCGTGGTGTGTGGCGGTGCCTGC100.1677008217340265No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAGGT100.1677008217340265No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAAA100.1677008217340265No Hit
GAGGAACGAGGAACACCTTACTCAGGA100.1677008217340265No Hit
TTGGCTGGCGTCGAGATGGTGATGAGGA100.1677008217340265No Hit
TCTCAAATCGTCGAGGTCGTGATAGAAAC100.1677008217340265No Hit
TGGCTGCTTTCTCAAATCCTCGAGGACGC90.15093073956062383No Hit
CACTGGCTGCTTTCCCAAATCGTCGAGGT90.15093073956062383No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAG90.15093073956062383No Hit
TAATGTGAGAGAATGAGCCTGGAGCGCCA90.15093073956062383No Hit
TTGTAATCATCTTCAAGTGCTGGACGCTCC90.15093073956062383No Hit
AACCACTGGCTGCTTTCCCAAATCGTCGAG90.15093073956062383No Hit
TACAACCACTGGCTGCTTTCTCAAATCGT90.15093073956062383No Hit
TCAGCGTGGTGTGTGGCGGTGCCTGCGGT90.15093073956062383No Hit
TCAGTCGGTAGAGCACC90.15093073956062383No Hit
TGATAGAAGACTCGAAGGTACCACTTCCGA80.13416065738722122No Hit
TTTATCAGCGTGGTGTGTGGCGGTGCCT80.13416065738722122No Hit
ACCACTGGCTGCTTTCCCAAATCGTCGAGG80.13416065738722122No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAT80.13416065738722122No Hit
TCATCATTGTGGCACTCTCTTTTCGAGATT80.13416065738722122No Hit
TGGCACTCTTGTGGAGATTCTCGGATGAG80.13416065738722122No Hit
CACTGGCTGCTTTCCCAAATCGTCGAGG80.13416065738722122No Hit
TAACATTTACAACTGGCTGCTTTCCCAAA80.13416065738722122No Hit
TTATCAGCGTGGTGTGTGGCGGTGCCT80.13416065738722122No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAGG80.13416065738722122No Hit
TTGTAATCATCTTCAAGTGCTGGGCGCTCC80.13416065738722122No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAG80.13416065738722122No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAGGT70.11739057521381856No Hit
TGTCAGCGTGGTGTGTGGCGGTGCCTGC70.11739057521381856No Hit
GAGGAACGAGGAACACCTTACTCAGGAG70.11739057521381856No Hit
TATCTCTAACTCTGATGGATGGATCTGCC70.11739057521381856No Hit
TCAAATCGTCGAGGACGCACTAGTAAGCA70.11739057521381856No Hit
CTGGCTGCTTTCTCAAATCGTCGAGGACGC70.11739057521381856No Hit
TGCTTTCCCAAATCGTCGAGGACGCACT70.11739057521381856No Hit
TCGACATAGCCCTTAACATTTACAACCACTG70.11739057521381856No Hit
TTTGTCAGCGTGGTGTGTGGCGGTGCCTGC70.11739057521381856No Hit
TGGCTGCTTTCTCAAATCGTCGAGGTCG60.1006204930404159No Hit
TATATGTTTGCACCCAACCCGATTGATGAC60.1006204930404159No Hit
TCGAAGAACACTTTCTCCCTCCCTAGGTA60.1006204930404159No Hit
TCAAATCGCCGAGGACGCACTAGTAAG60.1006204930404159No Hit
TCCCCTTTTGGAAGTTTGAACATGCCCTAGA60.1006204930404159No Hit
TCAAATCGCCGAGGACGCACTAGTAAGC60.1006204930404159No Hit
TGACATTATGACTGAATGGCGTGCTGCT60.1006204930404159No Hit
TGAAGATTTTCGTCGACTTGAGAGCCTCC60.1006204930404159No Hit
TACAACCACTGGCTGCTTTCTCAAATCCT60.1006204930404159No Hit
TCGTCGAGGACGCACTAGTAAGCAATACT60.1006204930404159No Hit
TGGCACTCTTGTTGAGATTCTCGGATG60.1006204930404159No Hit
CTCAAATCGTCGAGGACGCACTAGTAAGC60.1006204930404159No Hit
TTGGCTCTGTTGGCTGGCGTCGAGATGGT60.1006204930404159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)