FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orthomyxovirus.NODE_284.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orthomyxovirus.NODE_284.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length15-33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGCTGATTCGTTGCACCCTTTGAA48.88888888888889No Hit
TGGCGGCCTGTCCACGGGAAACGATTCAAA36.666666666666667No Hit
GAAACTAGTCGACTCCCTTGT24.444444444444445No Hit
ACCACGCTGACAAAAGAGTGATCCCCC24.444444444444445No Hit
TCTATCACATGTTGGACTTGACTGTCGTC24.444444444444445No Hit
AGCGAAACTAGTCGACTCCCT24.444444444444445No Hit
CTCCCTTGTATTTGTTGTAATTT24.444444444444445No Hit
AGAGTGATCCACCCACCCACTTGACAATG24.444444444444445No Hit
TGATGCATCCTGTAGGCTGAGTATTTGCT24.444444444444445No Hit
ACCACGCTGATAAAAGAGTGATCCACCCACC24.444444444444445No Hit
CCCTTTACTTGTGTGGACGA12.2222222222222223No Hit
ATGATTTCTACTGTACTTGACAAAGTATCCC12.2222222222222223No Hit
GTAAATGTTAAGGGCTATGTCGACAAAACAG12.2222222222222223No Hit
GACAAGAAGGTTGAT12.2222222222222223No Hit
GTTCCTCGTTCCTCATG12.2222222222222223No Hit
AACTAGTCGACTCCCTTGTATTTGT12.2222222222222223No Hit
CGAAACTAGTCGACTCCCTTGG12.2222222222222223No Hit
ACTAGTCGACTCCCTTGTATTTGT12.2222222222222223No Hit
TCCTCGACGATTTGAGAAAGCAGCCAGTGGTT12.2222222222222223No Hit
ATATCGATGAAAGTCTGTATGCTGCTGAC12.2222222222222223No Hit
ACCACGCTGACAAAAGAGTGATCCACCC12.2222222222222223No Hit
CGAGACACTTCTGCCACTTCTTTGGACAT12.2222222222222223No Hit
CGACTGAGCGAACTAGGCTGG12.2222222222222223No Hit
CTCTACCGACTGAGGTAACTAGGCTGG12.2222222222222223No Hit
TCCCTTGTATTTGTTGTAATTTCCCTGTGT12.2222222222222223No Hit
AAACTAGTCGACTCCCTTGTACC12.2222222222222223No Hit
TCGTGTGTGATAACTGTCGGAAGATTGT12.2222222222222223No Hit
AATAAGACGAGAGTCGGAAGTGGTAC12.2222222222222223No Hit
TGCTCTACCGACTGAG12.2222222222222223No Hit
GGTGCTCTACCGACTGAGCGAACTAGGCT12.2222222222222223No Hit
TGGCGGCCTGTCCACGGGAAACGATTCTTCAAT12.2222222222222223No Hit
ACCACGCTGACAAAAGAGTGATCCACCCAAA12.2222222222222223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)