FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orthomyxovirus.NODE_62727.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orthomyxovirus.NODE_62727.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length24-33
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGATCTAGCTTAGCCCTTTCTCCGTGT96.521739130434782No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGG85.797101449275362No Hit
TCTGAACCTCCTTGTAGCTCTTCTTTAGCACCA85.797101449275362No Hit
TCACTTGGTAAGCGGTTGCAGAAGGCACG53.6231884057971016No Hit
TCATAAATCTCTGACATCACTTGGTAAGC53.6231884057971016No Hit
TGTTCACCAATGACGCCCGATGCGACAGC53.6231884057971016No Hit
TCACCAATGACGCCCGATGCGACAGCGAAAT42.898550724637681No Hit
AGTGTTCACCAATGACGCCCGATGCGACAGC42.898550724637681No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTA42.898550724637681No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGGAT32.1739130434782608No Hit
TGAACCTCCTTGTAGCTCTTCTTTAGCACC32.1739130434782608No Hit
TAACCAAGAGGATTGGCATATTAAAAGGGG32.1739130434782608No Hit
TAACAGCGTTTGAGATGTGGTGCTTTCGT32.1739130434782608No Hit
TTAGAAAATAGCTTGCCCCGTGTTGGAAT32.1739130434782608No Hit
TCTGGAATCTTTGACTCTCTTCTGAACCT32.1739130434782608No Hit
TCTTTTTATTATGTTCGGAGAGGCTATGGC32.1739130434782608No Hit
TCTGGAATCTTTGACTCTCTTCTGAACC21.4492753623188406No Hit
TCTGAACCTCCTTGTAGCTCTTCTTTAGCACC21.4492753623188406No Hit
TCTGGAATCTTTGACTCTCTTCTGAACCTC21.4492753623188406No Hit
TTTCATAAATCTCTGACATCACTTGGTA21.4492753623188406No Hit
TTATTGTGGAGCCAGGCAGATATTTCCCA21.4492753623188406No Hit
TTCTTTTTGAAAGGACCTCTGGAATCTTT21.4492753623188406No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGGT21.4492753623188406No Hit
ATTTTTGTTAGAAAATGGCTTGCCCCGTG21.4492753623188406No Hit
TATTATGTTCGGAGAGGCTATGGCCCTG21.4492753623188406No Hit
TGATATTATTGGAGGCACACACGACGAA21.4492753623188406No Hit
TCATAAATCTCTGACATCACTTGGTAAGCG10.7246376811594203No Hit
AGTGTTCACCAATGACGCCCGATGCGAC10.7246376811594203No Hit
TTTCTTTTCTTCTCCGCCTATGCTTATTG10.7246376811594203No Hit
TTTTGTTAGAAAATAGCTTGCCCCGTGT10.7246376811594203No Hit
TTGGTAAGCGGTTGCAGAAGGCAC10.7246376811594203No Hit
ATGGAAAACCTTTCTCCGAACATTGTCCT10.7246376811594203No Hit
TGTTCACCAATGACGCCCGATGCGACAG10.7246376811594203No Hit
TAACCAAGAGGATTGGCATATTAAAAGGG10.7246376811594203No Hit
TTATTGTGGAGCCAGGCAGATATTTCCCAA10.7246376811594203No Hit
TTTAAAGGTAGGATGTTTTCAGATTTATAG10.7246376811594203No Hit
AATGGAAAACCTTTCTCCGAACATTGTCCT10.7246376811594203No Hit
AGCTTAGCCCTTTCTCCGTGTTTGATA10.7246376811594203No Hit
TTTCTTTTCTTCTCCGCCTATGCTTATT10.7246376811594203No Hit
TCACTTGGTAAGCGGTTGCAGAAGGC10.7246376811594203No Hit
TCAGGTGAAAGTCTTCTATTGCAAGGAG10.7246376811594203No Hit
GAACCTCCTTGTAGCTCTTCTTTAGCACCA10.7246376811594203No Hit
TGTCCTTTCCTTTTATCTTGACCCTCTTG10.7246376811594203No Hit
TTGGCATATTAAAAGGGGCTTTTTGGAAAC10.7246376811594203No Hit
TTAGCCCTTTCTCCGTGTTTGATATAACCGC10.7246376811594203No Hit
CAGTGTTCACCAATGACGCCCGATGCGACAG10.7246376811594203No Hit
TAAATCTCTGACATCACTTGGTAAGCGGT10.7246376811594203No Hit
TTCATAAATCTCTGACATCACTTGGTAAGC10.7246376811594203No Hit
AGTGTTCACCAATGACGCCCGATGCGACAGCGA10.7246376811594203No Hit
ACTTGGGCACAGTGTTCACCAATGACGCC10.7246376811594203No Hit
TCGGAAGCTACAAATACTTGGGCACAGTGA10.7246376811594203No Hit
TTTTGAAAGGACCTCTGGAATCTTTGACT10.7246376811594203No Hit
TAACAGCGTTTGAGATGTGGTGCTTT10.7246376811594203No Hit
TCATAAATCTCTGACATCACTTGGTAAGG10.7246376811594203No Hit
CAGTGTTCACCAATGACGCCCGATGCGAC10.7246376811594203No Hit
AGTAGTATGGGGTTTTTTTAAAGGT10.7246376811594203No Hit
TAGTAGTATGGGGTTTTTTTAAAGGTAGGA10.7246376811594203No Hit
TAACAGCGTTTGAGATGTGGTGCTTTC10.7246376811594203No Hit
TTGTGGAGCCAGGCAGATATTTCCCA10.7246376811594203No Hit
AGTGTTCACCAATGACGCCCGATGCGACA10.7246376811594203No Hit
TGCTTATTGTGGAGCCAGGCAGATATTTCC10.7246376811594203No Hit
TCATAAATCTCTGACATCACTTGGTAAG10.7246376811594203No Hit
TATGCTTATTGTGGAGCCAGGCAGATATT10.7246376811594203No Hit
TGTTAGAAAATAGCTTGCCCCGTGTTG10.7246376811594203No Hit
TGCTGCAGCGCGACCAATTACATATCATGT10.7246376811594203No Hit
TGTGGAGCCAGGCAGATATTTCCCAAGATT10.7246376811594203No Hit
TTCTCCGCCTATGCTTATTGTGGAGCCAGG10.7246376811594203No Hit
TAGCTTAGCCCTTTCTCCGTGTTTGATA10.7246376811594203No Hit
TGCGATCTAGCTTAGCCCTTTCTCCGTG10.7246376811594203No Hit
CGATCTAGCTTAGCCCTTTCTCCGTGTTT10.7246376811594203No Hit
TGAACCTCCTTGTAGCTCTTCTTTAGCACCA10.7246376811594203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)