FastQCFastQC Report
Wed 31 Mar 2021
S34.15-35nt.orthomyxovirus.NODE_62727.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.orthomyxovirus.NODE_62727.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length17-33
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTTTGTTTTCGTCGTGTGTGCCT842.10526315789473No Hit
CTGTTTGTTTTCGTCGTGTGTGCCT210.526315789473683No Hit
TGCCAATCCTCTTGGTTATTTCGCTGTC210.526315789473683No Hit
ACGGAGAAAGGGCTAAG15.263157894736842No Hit
AATTGGTCGCGCTGCAGCAAAGACACATAAGGC15.263157894736842No Hit
TGCCAATCCTCTTGGTTATTTCGCTGTCG15.263157894736842No Hit
TATGCCAATCCTCTTGGTTATTTCGCTGTC15.263157894736842No Hit
ACACGGAGAAAGGGCTAAGCTAGATCGC15.263157894736842No Hit
GTCTGTTTGTTTTCGTCGTGTGTGCCT15.263157894736842No Hit
TCTGTTTGTTTTCGTCGTGTGT15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)