FastQCFastQC Report
Mon 17 Jun 2024
S33.15-35nt.10628.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.10628.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229
Sequences flagged as poor quality0
Sequence length21-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACCTGAGCTTTGTACCACACAGACG86838.941229250785106No Hit
TAGAACCTGAGCTTTGTACCACACAGACGA944.217137729923732No Hit
TTCCAATACGCCCGTATGCCTGTTTGGCTC763.4096007178106778No Hit
TAGAACCTGAGCTTTGTACCACACAGACGAA703.140421713772992No Hit
TCCAATTGTAAAGCATCGACCCCCGAGTA562.512337371018394No Hit
TACCACTTCGTTCGCCTTACCTGTTGCCT391.7496635262449527No Hit
TCATCATACCACTTCGTTCGCCTTACCTG371.6599371915657246No Hit
TCCAATACGCCCGTATGCCTGTTTGGCTCC361.6150740242261103No Hit
TTCACAACCCGACTTCTCTGAATGCATTTC351.570210856886496No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGCTC351.570210856886496No Hit
TGCATTTCATCATACCACTTCGTTCGCCT301.3458950201884252No Hit
TCACAACCCGACTTCTCTGAATGCATTTCA301.3458950201884252No Hit
TCCAATTGTGAAGCATCGACCCCCGAGTA241.0767160161507403No Hit
TCACAACCCGACTTCTCTGAATGCATTTCAT231.031852848811126No Hit
TAGGGATATCTCTAGGCTGCCTTGCACAAG220.986989681471512No Hit
TCACAACCCGACTTCTCTGAATGCATTTC210.9421265141318977No Hit
TCCAATACGCCCGTATGCCTGTTTGGCTC200.8972633467922835No Hit
TAGGGATATCTCTAGGCTGCCTTGCACAA190.8524001794526694No Hit
TTAGGGATATCTCTAGGCTGCCTTGCACA160.7178106774338268No Hit
TAGGGATATCTCTAGGCTGCCTTGCACAAC140.6280843427545985No Hit
ATAGAACCTGAGCTTTGTACCACACAGACG120.5383580080753702No Hit
TGCTTCCAGTCTAGCCTCCTCCTGCTGCT110.493494840735756No Hit
TAGAACCTGAGCTTTGTACCACACAGA100.44863167339614174No Hit
TACGCCCGTATGCCTGTTTGGCTCCAGTCT100.44863167339614174No Hit
TAAATCCCAGCAGTAGCCAAGTGGTCTAGAC100.44863167339614174No Hit
TGGGACAGTATCCGATAGTTGAACCAGCT100.44863167339614174No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGCTCC100.44863167339614174No Hit
TTCAATGCATTTCACAACCCGACTTCTCT100.44863167339614174No Hit
TCCAATTGTAAAGCATCGACCCCCGAGT90.4037685060565276No Hit
TCATACCACTTCGTTCGCCTTACCTGTT90.4037685060565276No Hit
TCATCATACCACTTCGTTCGCCTTACCTGT90.4037685060565276No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGC80.3589053387169134No Hit
ATCCAATTGTAAAGCATCGACCCCCGAGT80.3589053387169134No Hit
TTCGCCACTTGACCCACAGTACGTGACTGTCC80.3589053387169134No Hit
TGCAAAGACAAATGTTCCAATACGCCCGT80.3589053387169134No Hit
TAGAACCTGAGCTTTGTACCACACAGAC70.31404217137729923No Hit
TCGCCACTTGACCCACAGTACGTGACTGTCC70.31404217137729923No Hit
TACCACTTCGTTCGCCTTACCTGTTGCCTC70.31404217137729923No Hit
TTCATCATACCACTTCGTTCGCCTTACCTG70.31404217137729923No Hit
TGTTGATGTCGAGGACCTCGAAGTTGAG60.2691790040376851No Hit
AGAACCTGAGCTTTGTACCACACAGACG60.2691790040376851No Hit
TTCATCATACCACTTCGTTCGCCTTACCT60.2691790040376851No Hit
TCCATTGCTTCCAGTCTAGCCTCCTCCTGC60.2691790040376851No Hit
TTGGCTCCAGTCTAGCCTCCTCCTGCTGCT60.2691790040376851No Hit
TCCAATACGCCCGTATGCCTGTTTGGCTCCA50.22431583669807087No Hit
TCCAATTGTAAAGCATCGACCCCCGAGTAT50.22431583669807087No Hit
TAGAACCTGAGCTTTGTACCACACAGACGAATGCA50.22431583669807087No Hit
CCAATTGTAAAGCATCGACCCCCGAGTA50.22431583669807087No Hit
TCCCACGACCCAAATTGGTCCGAGTACCAC50.22431583669807087No Hit
TCATACAGATCACCTGCCACGATAAACTG50.22431583669807087No Hit
AAGGTCTTCCTGATCCCAGGAGTAGGTC50.22431583669807087No Hit
TTCACAACCCGACTTCTCTGAATGCATTTCAT50.22431583669807087No Hit
TGTAAAGCATCGACCCCCGAGTATTCGAGT50.22431583669807087No Hit
TCCCACGACCCAAATTGGTCCGAGTACCACTC50.22431583669807087No Hit
TCACAACCCGACTTCTCTGAATGCATTTCATC40.1794526693584567No Hit
TAGGGATATCTCTAGGCTGCCTTGCAC40.1794526693584567No Hit
TCATACCACTTCGTTCGCCTTACCTGTTGC40.1794526693584567No Hit
TCCTGCTGCTGCAAAGACAAATGTTCCAAT40.1794526693584567No Hit
TGTATATGACCTCAGCTCCGAGTACCCCT40.1794526693584567No Hit
TGCTTCCAGTCTAGCCTCCTCCTGCTGC40.1794526693584567No Hit
TTCCAATACGCCCGTATGCCTGTTTGGCTCC40.1794526693584567No Hit
TAGGGATATCTCTAGGCTGCCTTGCACA40.1794526693584567No Hit
TCTAGACCGTTGTTACTCCACGTTCCCGG40.1794526693584567No Hit
TACCATTCGCCACTTGACCCACAGTACGT40.1794526693584567No Hit
TTTTGTTGTATCTTTCGTTTCACCCCGGGC40.1794526693584567No Hit
TCGCCACTTGACCCACAGTACGTGACTGTC40.1794526693584567No Hit
TGTAAAGCATCGACCCCCGAGTATTCGAG40.1794526693584567No Hit
TATTCGACCACACACCTTCGATAGTCCAGT40.1794526693584567No Hit
TAACGATGTATATGACCTCAGCTCCGAGT30.13458950201884254No Hit
TACCACTTCGTTCACCTTACCTGTTGCCT30.13458950201884254No Hit
TTATTCGTTACAGACCAGCTCAGTTCCGAC30.13458950201884254No Hit
TCACAACTTGGGGCGCAGGGGACAAAGT30.13458950201884254No Hit
TCAATGCATTTCACAACCCGACTTCTCT30.13458950201884254No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGG30.13458950201884254No Hit
TTCTCCATGAAACATTAACGAGTTCCTCGTC30.13458950201884254No Hit
TTCGTTACAGACCAGCTCAGTTCCGACT30.13458950201884254No Hit
TAGACCGTTGTTACTCCACGTTCCCGGTT30.13458950201884254No Hit
TGCACAAGCACTCCGTCCCACGACCCAAAT30.13458950201884254No Hit
TAGAACCTGAGCTTTGTACCACACAGAAG30.13458950201884254No Hit
TGCATTTCATCATACCACTTCGTTCGCC30.13458950201884254No Hit
TTTTTTTTGGGGGCATTCACAACTTGGGG30.13458950201884254No Hit
TGATGTCGAGGACCTCGAAGTTGAGAGAG30.13458950201884254No Hit
TAGGCTGCCTTGCACAACCACTCCGTCCCAC30.13458950201884254No Hit
TCCATTGCTTCCAGTCTAGCCTCCTCCTGT30.13458950201884254No Hit
TCCTCCTGCTGCTGCAAAGACAAATGTTCC30.13458950201884254No Hit
CGCGAGAGATGTCAAGGTAGTACTCAGCT30.13458950201884254No Hit
TCCCACGACCCAAATTGGTCCGAGTACCACT30.13458950201884254No Hit
TTTTTTTGGGGCATTCACAACTTGGGGC30.13458950201884254No Hit
TAGGGATATCTCTAGGCTGCCTTGCACAAA30.13458950201884254No Hit
TCTTGGTTGATACGTAATATGTTTTTAGGG30.13458950201884254No Hit
TTCAATGCATTTCACAACCCGACTTCTCTC30.13458950201884254No Hit
TGTGAAGCATCGACCCCCGAGTATTCGAG30.13458950201884254No Hit
TAACGATGTATATGACCTCAGCTCCGAGTA30.13458950201884254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)