FastQCFastQC Report
Mon 17 Jun 2024
S33.15-35nt.284.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.284.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6046
Sequences flagged as poor quality0
Sequence length16-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACGAGGAACACCTTACTCAGGAGGA321853.22527290770758No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAGC1903.14257360238174No Hit
AACCACTGGCTGCTTTCTCAAATCGTCGAG1222.0178630499503805No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAA1121.8524644392987097No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAGG961.587826662256037No Hit
TCGACATAGCCCTTAACATTTACAACCACT841.3893483294740323No Hit
GAGGAACGAGGAACACCTTACTCAGGAGG661.0916308303010256No Hit
TGGCACTCTTGTTGAGATTCTCGGATGAG570.9427720807145219No Hit
TCAAATCGTCGAGGACGCACTAGTAAGC560.9262322196493549No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGC430.7112140258021833No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAG410.6781343036718491No Hit
CCACTGGCTGCTTTCTCAAATCGTCGAGG400.6615944426066821No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAGC390.6450545815415151No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTC360.5954349983460139No Hit
TCAAATCGTCGAGGACGCAGTAGTAAGC340.5623552762156798No Hit
TAACATTTACAACCACTGGCTGCTTTCTC320.5292755540853458No Hit
AGGAACGAGGAACACCTTACTCAGGAGGA310.5127356930201786No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAG290.47965597088984446No Hit
TCGTCGAGGTCGCACTAGTAAGCAATACT250.4134965266291763No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAGT220.36387694343367516No Hit
TTCTCAAATCGCCGAGGACGCACTAGTAAGC200.33079722130334105No Hit
TGGCACTCTTGTGGAGATTCTCGGATGAG200.33079722130334105No Hit
TAGCTGCTTTCTCAAATCGTCGAGGACGC200.33079722130334105No Hit
TCCCAAATCGTCGAGGACGCACTAGAAAGC190.314257360238174No Hit
TCCGACTCTCGTCTTATTCTGTACCTTCCT190.314257360238174No Hit
TTAACATTTACAACCACTGGCTGCTTTCTC180.29771749917300694No Hit
TGTAATCATCTTCAAGTGCTGGGCGCTCC160.2646377770426729No Hit
TTGTAATCATCTTCAAGTGCTGGGCGCTCC150.24809791597750577No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAG140.23155805491233872No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAG140.23155805491233872No Hit
TCAAATCGTCGAGGTCGTGATAGAAACCA140.23155805491233872No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAGC130.21501819384717166No Hit
TCAAATCGTCGAGGACGCACTAGTAAG120.19847833278200464No Hit
TAACATTTACAACTGGCTGCTTTCCCAAA120.19847833278200464No Hit
TTTGGCCTTCCCGCCAGCCCATCCACCTT120.19847833278200464No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAG120.19847833278200464No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAG120.19847833278200464No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAAAG110.18193847171683758No Hit
TGCTTTCTCAAATCGTCGAGGACGCACT110.18193847171683758No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAG110.18193847171683758No Hit
CACTGGCTGCTTTCCCAAATCGTCGAGGT100.16539861065167052No Hit
TTGTAATCATCTTCAAGTGCTGGGCGCTC100.16539861065167052No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGCA100.16539861065167052No Hit
TACATGCAGCCCATTTGGCCTTCCCGCCAGC100.16539861065167052No Hit
TTGGCTCTGTTGGCTGGCGTCGAGATGGT100.16539861065167052No Hit
TTACAACCACTGGCTGCTTTCTCAAATCGTCGAG100.16539861065167052No Hit
TGCATGCAGCCCATTTGGCCTTCCCGCCAGC90.14885874958650347No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAAA90.14885874958650347No Hit
TCGGGAATTCTCCCTCGCCAAAGCCGGAT90.14885874958650347No Hit
TTACAACCACTGGCTGCTTTCTCAAATC80.13231888852133644No Hit
CTGGCTGCTTTCTCAAATCGTCGAGGACGC80.13231888852133644No Hit
TCTGTTTTGTCGACATAGCCCTTAACATT80.13231888852133644No Hit
TGAGAATATCTCTAACTCTGATGGATGGA80.13231888852133644No Hit
TACAACCACTGGCTGCTTTCTCAAATCGT80.13231888852133644No Hit
TGCTTTCTCAAATCGTCGAGGTCGTGAT80.13231888852133644No Hit
GAGGAACGAGGAACACCTTACTCAGGAG80.13231888852133644No Hit
TCTGTACGGAAAGGTTGGCGGGTTGCCGGC80.13231888852133644No Hit
TCGTCGAGGACGCAGTAGTAAGCAATACTT80.13231888852133644No Hit
TCCCGATCCACCAATCTAGAGCCTCTCTCT80.13231888852133644No Hit
TTATCAGCGTGGTGTGTGGCGGTGCCTGC80.13231888852133644No Hit
TACAACCACTGGCTGCTTTCTCAAATCGCCGAG80.13231888852133644No Hit
CAACTGGCTGCTTTCCCAAATCGTCGAGGT80.13231888852133644No Hit
TCAGTTTTGTCGACATAGCCCTTAACATT80.13231888852133644No Hit
TGTAATGTGAGAGAATGAGCCTGGAGCGCC80.13231888852133644No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAGGT80.13231888852133644No Hit
TATGTTTGCACCCAACCCGATTGATGAC80.13231888852133644No Hit
TTCTCAAATCGTCGAGGTCGTGATAGAAAC70.11577902745616936No Hit
TGTGATAGAAGACTCGAAGGTACCACTTCC70.11577902745616936No Hit
TGATACTCGAAGAACACTTTCTCCCTCCCT70.11577902745616936No Hit
CTCAAATCGCCGAGGACGCACTAGTAAGC70.11577902745616936No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAGGT70.11577902745616936No Hit
TACCACTTCCGACTCTCGTCTTATTCTGT70.11577902745616936No Hit
TCTCAAATCCTCGAGGACGCACTAGTAAGC70.11577902745616936No Hit
TCTCAAATCGTCGAGGTCGTGATAGAAACC70.11577902745616936No Hit
TAACTTTCCGCCCCGCCCTTAGCAAAAG70.11577902745616936No Hit
TTTGTCAGCGTGGTGTGTGGCGGTGCCT70.11577902745616936No Hit
TGAGGAGACTCTTGATGACAGATTTGCCT70.11577902745616936No Hit
TGAGGAACGAGGAACACCTTACTCAGGAGGA70.11577902745616936No Hit
TCCCAAATCGTCGAGGTCGTGATAGAAACC70.11577902745616936No Hit
TCTCAAATCGTCGAGGTCGTGATAGAAAC70.11577902745616936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)