FastQCFastQC Report
Mon 17 Jun 2024
S33.15-35nt.284.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.284.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length19-34
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAATGTTAAGGGCTATGTCGACAAAACAGAAC325.0No Hit
TCCGGCTTTGGCGAGGGACAATTCCCGGTA18.333333333333332No Hit
GCCCAATATATGATCGCTAACAATGTCCCTCT18.333333333333332No Hit
ATTTCCACCCCCCTCCTGC18.333333333333332No Hit
GAAACTAGTCGACTCCCTTGTATTTTCCCT18.333333333333332No Hit
TTCTTTCCATCATGATTTCCACTG18.333333333333332No Hit
TCAACTTGTAAAACTTCTAGGGCATGT18.333333333333332No Hit
AAACTAGTCGACTCCCTTGTATTTGTTGTATT18.333333333333332No Hit
TATCTCCATGGCTCATAGTTGAACTCTGC18.333333333333332No Hit
CTACATACGAAGTCCTCATGCGCCT18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)