FastQCFastQC Report
Mon 17 Jun 2024
S34.15-35nt.10628.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.10628.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259
Sequences flagged as poor quality0
Sequence length16-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAACCTGAGCTTTGTACCACACAGACG88439.132359451084554No Hit
TAGAACCTGAGCTTTGTACCACACAGACGAA1386.108897742363878No Hit
TAGAACCTGAGCTTTGTACCACACAGACGA984.338202744577247No Hit
TACCACTTCGTTCGCCTTACCTGTTGCCT883.8955289951305887No Hit
TCATCATACCACTTCGTTCGCCTTACCTG773.408587870739265No Hit
TGCATTTCATCATACCACTTCGTTCGCCT672.9659141212926072No Hit
TTCCAATACGCCCGTATGCCTGTTTGGCTC622.7445772465692784No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGCTC472.080566622399292No Hit
TCATCATACCACTTCGTTCGCCTTACCTGT431.9034971226206288No Hit
TAGAACCTGAGCTTTGTACCACACAGAC381.6821602478973No Hit
TCATACCACTTCGTTCGCCTTACCTGTTG251.1066843736166445No Hit
TCCAATACGCCCGTATGCCTGTTTGGCTC231.018149623727313No Hit
ATAGAACCTGAGCTTTGTACCACACAGACG231.018149623727313No Hit
TCCAATACGCCCGTATGCCTGTTTGGCTCC210.9296148738379815No Hit
TACGCCCGTATGCCTGTTTGGCTCCAGTCT200.8853474988933155No Hit
TTCATCATACCACTTCGTTCGCCTTACCTG170.7525453740593183No Hit
TCATACCACTTCGTTCGCCTTACCTGTTGC160.7082779991146525No Hit
TAGGGATATCTCTAGGCTGCCTTGCACAAC150.6640106241699867No Hit
TCCAATTGTGAAGCATCGACCCCCGAGTA140.619743249225321No Hit
TACCACTTCGTTCGCCTTACCTGTTGCCTC140.619743249225321No Hit
TAGAACCTGAGCTTTGTACCACACAGA130.5754758742806552No Hit
AGAACCTGAGCTTTGTACCACACAGACG130.5754758742806552No Hit
CATTGCTTCCAGTCTAGCCTCCTCCTGCT130.5754758742806552No Hit
TCATACCACTTCGTTCGCCTTACCTGTT100.44267374944665777No Hit
TTCATCATACCACTTCGTTCGCCTTACCT100.44267374944665777No Hit
TCAATGCATTTCACAACCCGACTTCTCTC80.35413899955732625No Hit
TTCCAATACGCCCGTATGCCTGTTTGGCTCC80.35413899955732625No Hit
TCATACCACTTCGTTCGCCTTACCTG70.3098716246126605No Hit
TCCCAGGAGTAGGTCATTCTGTTGACATA70.3098716246126605No Hit
TGCACAACCACTCCGTCCCACGACCCAAAT60.2656042496679947No Hit
TCTAGCCTCCTGCTGCTGCTGCAAAGAC60.2656042496679947No Hit
TCCATTGCTTCCAGTCTAGCCTCCTGCTGC60.2656042496679947No Hit
TCCATTGCTTCCAGTCTAGCCTCCTCCTG60.2656042496679947No Hit
TTGGCTCCAGTCTAGCCTCCTCCTGCTGCT60.2656042496679947No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGC50.22133687472332889No Hit
TTCCATTGCTTCCAGTCTAGCCTCCTGC50.22133687472332889No Hit
TCTTGTCGTTGAGTTAGGTGCCAGGCCATC50.22133687472332889No Hit
TGCTTCCAGTCTAGCCTCCTCCTGCTGCT50.22133687472332889No Hit
TTAGGGATATCTCTAGGCTGCCTTGCACA50.22133687472332889No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGCTCC50.22133687472332889No Hit
TGCATGAGCAGACATGATGTGGGACAGT50.22133687472332889No Hit
TCCAATACGCCCGTATGCCTGTTTGGCTCCA40.17706949977866313No Hit
TCATACCACTTCGTTCGCCTTACCTGT40.17706949977866313No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGG40.17706949977866313No Hit
TAGGGATATCTCTAGGCTGCCTTGCACAA40.17706949977866313No Hit
TTCGTTTCACCCCGGGCCAATGGTCTTCC40.17706949977866313No Hit
TGCATTTCATCATACCACTTCGTTCGCC40.17706949977866313No Hit
TTCACAACCCGACTTCTCTCAATGCATTTC40.17706949977866313No Hit
TACCACTTCGTTCGCCTTACCTGTTGCC40.17706949977866313No Hit
TAGAACCTGAGCTTTGTACCACACAGACGG40.17706949977866313No Hit
TCTAAGTCTGACGTGGTATAAATCCCAGC40.17706949977866313No Hit
TGCTTCCAGTCTAGCCTCCTGCTGCTGCT40.17706949977866313No Hit
TCATCATACCACTTCGTTCGCCTTACCTGTT40.17706949977866313No Hit
TTCCATTGCTTCCAGTCTAGCCTCCTCCT40.17706949977866313No Hit
TTCAATGCATTTCACAACCCGACTTCTCTC40.17706949977866313No Hit
TCTAGACCGTTGTTACTCCACGTTCCCG30.13280212483399734No Hit
TCCTGCTGCTGCTGCAAAGACAAATGTTCC30.13280212483399734No Hit
TCCTCCTGCTGCTGCAAAGACAAATGTTCCAAT30.13280212483399734No Hit
TTCAATGCATTTCACAACCCGACTTCTC30.13280212483399734No Hit
TAGAACCTGAGCTTTGTACCACACA30.13280212483399734No Hit
TGTTCCAATACGCCCGTATGCCTGTTTGGCT30.13280212483399734No Hit
TTCATCATACCACTTCGTTCGCCTTACCTGT30.13280212483399734No Hit
TCTACAGGATGCGAACGAGCAGTAGCTTGGA30.13280212483399734No Hit
TGATGTCGAGGACCTCGAAGTTGAGAGAG30.13280212483399734No Hit
TGCACAACCACTCCGTCCCACGACCCAA30.13280212483399734No Hit
TCCATTGCTTCCAGTCTAGCCTCCTCCTGC30.13280212483399734No Hit
TCATCATACCACTTCGTTCGCCTTACCTGTTGCC30.13280212483399734No Hit
TTCCAGTCTAGCCTCCTCCTGCTGCTGC30.13280212483399734No Hit
TTCGAGTTGGAACCCTTTCCTCCCTTTCT30.13280212483399734No Hit
TTCGAGTTGGAACCCTTTCCTCCCTTTCA30.13280212483399734No Hit
TCCTCCTGCTGCTGCAAAGACAAATGTTCC30.13280212483399734No Hit
TGCTTCCAGTCTAGCCTCCTCCTGCTGC30.13280212483399734No Hit
TAGACCGTTGTTACTCCACGTTCCCGGT30.13280212483399734No Hit
TTCCATTGCTTCCAGTCTAGCCTCCT30.13280212483399734No Hit
CACTTCGTTCGCCTTACCTGTTGCCTCTT30.13280212483399734No Hit
TCACAACCCGACTTCTCTCAATGCATTTC30.13280212483399734No Hit
TACCACTTCAATGCATTTCACAACCCGACT30.13280212483399734No Hit
TAACGATGTATATGACCTCAGCTCCGAGTA30.13280212483399734No Hit
TTCAATGCATTTCACAACCCGACTTCTCT30.13280212483399734No Hit
TTGTATGCGACGAGGCCATAACAGAGAG30.13280212483399734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)