FastQCFastQC Report
Mon 17 Jun 2024
S34.15-35nt.284.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS34.15-35nt.284.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6666
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACGAGGAACACCTTACTCAGGAGGA238635.7935793579358No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAGC2503.7503750375037503No Hit
AACCACTGGCTGCTTTCTCAAATCGTCGAG1422.13021302130213No Hit
TCGACATAGCCCTTAACATTTACAACCACT1221.8301830183018304No Hit
CCACTGGCTGCTTTCTCAAATCGTCGAGG1121.6801680168016802No Hit
TGGCACTCTTGTTGAGATTCTCGGATGAG1081.6201620162016201No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAA1071.6051605160516051No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAGG1041.5601560156015601No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTC660.9900990099009901No Hit
TCAAATCGTCGAGGACGCACTAGTAAGC630.9450945094509451No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAGC610.9150915091509152No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGC590.8850885088508851No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAGT500.7500750075007501No Hit
GAGGAACGAGGAACACCTTACTCAGGAGG470.7050705070507051No Hit
TAGCTGCTTTCTCAAATCGTCGAGGACGC470.7050705070507051No Hit
TCAAATCGTCGAGGACGCAGTAGTAAGC450.6750675067506751No Hit
TCTCAAATCGCCGAGGACGCACTAGTAAG440.6600660066006601No Hit
TCTCAAATCGTCGAGGACGCACTAGTAAG420.6300630063006301No Hit
TAACATTTACAACCACTGGCTGCTTTCTC380.57005700570057No Hit
TGCTTTCTCAAATCGTCGAGGACGCACT300.45004500450045004No Hit
TCTCAAATCCTCGAGGACGCACTAGTAAGC280.42004200420042004No Hit
TCCCAAATCGTCGAGGTCGTGATAGAAACC250.37503750375037503No Hit
TTCTCAAATCGCCGAGGACGCACTAGTAAGC240.36003600360036003No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAGG240.36003600360036003No Hit
CCACTGGCTGCTTTCCCAAATCGTCGAGG240.36003600360036003No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAGC240.36003600360036003No Hit
AGGAACGAGGAACACCTTACTCAGGAGGA240.36003600360036003No Hit
TGTAATCATCTTCAAGTGCTGGGCGCTCC230.34503450345034503No Hit
TCCCAAATCGTCGAGGACGCACTAGAAAG230.34503450345034503No Hit
TGCTTTCTCAAATCGTCGAGGTCGTGAT220.33003300330033003No Hit
ACCACTGGCTGCTTTCTCAAATCGTCGAG220.33003300330033003No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAG220.33003300330033003No Hit
TCGTCGAGGTCGCACTAGTAAGCAATACT210.31503150315031503No Hit
TCTGTTTTGTCGACATAGCCCTTAACATT200.3000300030003No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAGGT200.3000300030003No Hit
TCCCAAATCGTCGAGGACGCACTAGAAAGC200.3000300030003No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGGA190.285028502850285No Hit
TATGTTTGCACCCAACCCGATTGATGAC190.285028502850285No Hit
CACTGGCTGCTTTCCCAAATCGTCGAGGT180.27002700270027No Hit
TGGCTGCTTTCTCAAATCGTCGAGGACGCA180.27002700270027No Hit
TACAACTGGCTGCTTTCCCAAATCGTCGAG180.27002700270027No Hit
AACCACTGGCTGCTTTCCCAAATCGTCGAG180.27002700270027No Hit
TACAACCACTGGCTGCTTTCTCAAATCGT180.27002700270027No Hit
ACCACTGGCTGCTTTCCCAAATCGTCGAGG160.24002400240024005No Hit
TTCTCAAATCGTCGAGGACGCACTAGTAAGC160.24002400240024005No Hit
TCGACTTGAGAGCCTCCACAGCAGCAGAG160.24002400240024005No Hit
CACTGGCTGCTTTCCCAAATCGTCGAGG160.24002400240024005No Hit
TAACATTTACAACTGGCTGCTTTCCCAAA160.24002400240024005No Hit
TCCCAAATCGTCGAGGACGCACTAGTAAGC160.24002400240024005No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAGG160.24002400240024005No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAC150.22502250225022502No Hit
TAACTTTCCGCCCCGCCCTTAGCAAAAG150.22502250225022502No Hit
TCAACAGCTTTCCCAAATCGTCGAGGACGC140.21002100210021002No Hit
TGCATGCAGCCCATTTGGCCTTCCCGCCAGC140.21002100210021002No Hit
TCGACATAGCCCTTAACATTTACAACCACTG140.21002100210021002No Hit
TCCGACTCTCGTCTTATTCTGTACCTTCCT140.21002100210021002No Hit
GCTGGCACTCTTGTTGAGATTCTCGGATG130.19501950195019502No Hit
TTTATCAGCGTGGTGTGTGGCGGTGCCTGC130.19501950195019502No Hit
TTTGTCAGCGTGGTGTGTGGCGGTGCCT130.19501950195019502No Hit
TGATACTCGAAGAACACTTTCTCCCTCCCT120.18001800180018002No Hit
TCAAATCGTCGAGGACGCACTAGTAAG120.18001800180018002No Hit
TGGCACTCTTGTTGAGATTCTCGGATGA120.18001800180018002No Hit
TGAGGAGACTCTTGATGACAGATTTGCCT120.18001800180018002No Hit
TACAACCACTGGCTGCTTTCTCAAATCCT120.18001800180018002No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGGT120.18001800180018002No Hit
CCACTGGCTGCTTTCTCAAATCGTCGAGGT120.18001800180018002No Hit
TCAAATCGTCGAGGTCGTGATAGAAACCA120.18001800180018002No Hit
TTGGGACTTGGGCGTTAGTCTGCTTGGCT110.16501650165016502No Hit
TAAGATGACCCTCACTCTGATAGATGGAT110.16501650165016502No Hit
TACATGCAGCCCATTTGGCCTTCCCGCCAGC110.16501650165016502No Hit
TTTGGCCTTCCCGCCAGCCCATCCACCTT100.15001500150015No Hit
TTATCAGCGTGGTGTGTGGCGGTGCCTGC100.15001500150015No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAAA100.15001500150015No Hit
GAGGAACGAGGAACACCTTACTCAGGA100.15001500150015No Hit
TTGGCTGGCGTCGAGATGGTGATGAGGA100.15001500150015No Hit
TCTCAAATCGTCGAGGTCGTGATAGAAAC100.15001500150015No Hit
TGGCTGCTTTCTCAAATCCTCGAGGACGC90.135013501350135No Hit
TCCCAAATCGTCGAGG90.135013501350135No Hit
TAATGTGAGAGAATGAGCCTGGAGCGCCA90.135013501350135No Hit
TTGTAATCATCTTCAAGTGCTGGACGCTCC90.135013501350135No Hit
TCAGCGTGGTGTGTGGCGGTGCCTGCGGT90.135013501350135No Hit
TGTTCTGTTTTGTCGACATAGCCCTTAAC90.135013501350135No Hit
TCAGTCGGTAGAGCACC90.135013501350135No Hit
TAACATTTACAACCACTAGCTGCTTTCTC80.12001200120012002No Hit
CACTGGCTGCTTTCTCAAATCGTCGAGG80.12001200120012002No Hit
AACTGGCTGCTTTCCCAAATCGTCGAGGT80.12001200120012002No Hit
TACAAGTGTTCTGTTTTGTAGACATAGCCCT80.12001200120012002No Hit
TGATAGAAGACTCGAAGGTACCACTTCCGA80.12001200120012002No Hit
TTTGGCCTTCCCGCCAGCCCATCCACCT80.12001200120012002No Hit
TTTATCAGCGTGGTGTGTGGCGGTGCCT80.12001200120012002No Hit
AACCACTAGCTGCTTTCTCAAATCGTCGAG80.12001200120012002No Hit
GAGGAACGAGGAACACCTTACTCAGGAGGAT80.12001200120012002No Hit
TCATCATTGTGGCACTCTCTTTTCGAGATT80.12001200120012002No Hit
TGTCGACATAGCCCTTAACATTTACAACCAC80.12001200120012002No Hit
TGGCACTCTTGTGGAGATTCTCGGATGAG80.12001200120012002No Hit
TTAACATTTACAACTGGCTGCTTTCCCAAA80.12001200120012002No Hit
TTATCAGCGTGGTGTGTGGCGGTGCCT80.12001200120012002No Hit
ACCACTGGCTGCTTTCCCAAATCGTCGAG80.12001200120012002No Hit
TTGTAATCATCTTCAAGTGCTGGGCGCTCC80.12001200120012002No Hit
CAACTGGCTGCTTTCCCAAATCGTCGAGGT80.12001200120012002No Hit
TCAGTTTTGTCGACATAGCCCTTAACATT80.12001200120012002No Hit
TCTCAAATCGTCGAGGACGCAGTAGTAAG80.12001200120012002No Hit
TACAACCACTAGCTGCTTTCTCAAATCGTC80.12001200120012002No Hit
TGCAGCCCATTTGGCCTTCCCGCCAGCCC80.12001200120012002No Hit
TACAACCACTGGCTGCTTTCTCAAATCGTCGAGGT70.10501050105010501No Hit
TGTCAGCGTGGTGTGTGGCGGTGCCTGC70.10501050105010501No Hit
GAGGAACGAGGAACACCTTACTCAGGAG70.10501050105010501No Hit
TATCTCTAACTCTGATGGATGGATCTGCC70.10501050105010501No Hit
TCAAATCGTCGAGGACGCACTAGTAAGCA70.10501050105010501No Hit
CTGGCTGCTTTCTCAAATCGTCGAGGACGC70.10501050105010501No Hit
TGCTTTCCCAAATCGTCGAGGACGCACT70.10501050105010501No Hit
TTTGTCAGCGTGGTGTGTGGCGGTGCCTGC70.10501050105010501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)