FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.12121.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.12121.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624
Sequences flagged as poor quality0
Sequence length17-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGGATCCCGGCCTAAGCTCGCATAGC15424.679487179487182No Hit
TTGAAGGATCCCGGCCTAAGCTCGCATAGC294.647435897435898No Hit
TGAAACAGGATTCCGGCTGGGTACTTCTC132.083333333333333No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGCTTCT101.6025641025641024No Hit
TAATTAAGTGGGTTAGGGCTTGTCTCTTC101.6025641025641024No Hit
AAGGATCCCGGCCTAAGCTCGCATAGCTTCT101.6025641025641024No Hit
TGAACAGCCCAGGGTTTTGATCTAAAATGGT91.4423076923076923No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGCT91.4423076923076923No Hit
TCCCACGATGGCCAAGTACAATAAATATAGC91.4423076923076923No Hit
TATGTCCATTTGCAGCCAGTGTACCACCAC81.282051282051282No Hit
TACTTCTCTGCCAACTGATTGATCAGT81.282051282051282No Hit
TTTGAAGGATCCCGGCCTAAGCTCGCATAGCTTCT60.9615384615384616No Hit
TGAGAGAATCAGACCCCTTATAGACCAAG60.9615384615384616No Hit
TGTTCCATCCAAACAGTACGCCCTGTGGAATT60.9615384615384616No Hit
TTTGAAGGATCCCGGCCTAAGCTCGCA60.9615384615384616No Hit
TTTGAAGGATCCCGGCCTAAGCTCGCATA60.9615384615384616No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAG50.8012820512820512No Hit
TTTGAAGGATCCCGGCCTAAGCTCGCATAGC50.8012820512820512No Hit
TGTAACATTTGTCGTCGAGGGGGAGAGC50.8012820512820512No Hit
GGAGACCTTGGAAAAATTGAG50.8012820512820512No Hit
AAGGATCCCGGCCTAAGCTCGCATAGCTTC50.8012820512820512No Hit
TTCAACCCTAAGTGCTTGTCCTGTCCCCAAC40.641025641025641No Hit
TTAACATTGGACTCACGAGATTTGAAGAGC40.641025641025641No Hit
TCTTGTACTGCACTGGCGATATTTTCAAAC40.641025641025641No Hit
TCACCGCGAACTACAGCATTGAATCCAGAG40.641025641025641No Hit
TCTCCTTCCCTTGTTCTGTGATGATCCGT40.641025641025641No Hit
TGTCCGCAGGGAGTGAAAGCCACATACTG40.641025641025641No Hit
TACAATGGTTTGAGAAAGGGGTCGCACTC40.641025641025641No Hit
TAGTACAATGGTTTGAGAAAGGGGTCGCA40.641025641025641No Hit
TGTAACATTTGTCGTCGAGGGGGAGAGCGT30.4807692307692308No Hit
TCAGACTCCGGCGTTGTCATTCCTTTCTT30.4807692307692308No Hit
TACCATTCAACCCTAAGTGCTTGTCCTGT30.4807692307692308No Hit
TCCCCAACTGGAATTCGATTTCAAGAGATG30.4807692307692308No Hit
TCCTCTGGGCTCATTTTGGCTAAAGTCAACA30.4807692307692308No Hit
TTTGAAGAGCTCATACAGTCGATACAAGAC30.4807692307692308No Hit
TTCTGTGATGATCCGTGACCTAGGTGAC30.4807692307692308No Hit
TGTAACATTTGTCGTCGAGGGGGAGAGCG30.4807692307692308No Hit
TACAATGGTTTGAGAAAGGGGTCGCACT30.4807692307692308No Hit
TGTAACATTTGTCGCCGAGGGGGAGAGCGT30.4807692307692308No Hit
TTGCCTTAGGCAATCTCTACCATTCAACCCTG30.4807692307692308No Hit
TGAAAGCCACATACTGTCACCGCTCCCTC20.3205128205128205No Hit
GGAGACCTTGGAAAAATTGAGTGGGAATAGG20.3205128205128205No Hit
ACATACTGTCACCGCTCCCTCTATGACC20.3205128205128205No Hit
TCATTGTAACATTTGTCGTCGAGGGGGA20.3205128205128205No Hit
TAACATTTGTCGTCGAGGGGGAGAGCGT20.3205128205128205No Hit
TCGATACGAGCTTTCGTCCAGTAGTTTTC20.3205128205128205No Hit
TTTGCAGCCAGTGTACCACCACTCGTACAGG20.3205128205128205No Hit
TTTTGAAGGATCCCGGCCTAAGCTCGCA20.3205128205128205No Hit
TTTCAAGAGATGTGACTCTCACGGCCCT20.3205128205128205No Hit
TGTAACATTTGTCGTCGAGGGGGAGAG20.3205128205128205No Hit
TACTGTCACCGCTCCCTCTATGACCTGAA20.3205128205128205No Hit
CAGTCCAAGGTGCTCTGTCCGCAGGGAGT20.3205128205128205No Hit
CACCGCGAACTACAGCATTGAATCCAGAG20.3205128205128205No Hit
GAAGGATCCCGGCCTAAGCTCGCATAGCTT20.3205128205128205No Hit
CTTGGAGACCTTAAAACACGACGTTTGG20.3205128205128205No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGCTTC20.3205128205128205No Hit
TACTGGTACGCAAAGTCCTCTGGGCTCAT20.3205128205128205No Hit
TCCATTTGCAGCCAGTGTACCACCACTCGT20.3205128205128205No Hit
ACAGTAAATATTATACAATCAAAGGGCGA20.3205128205128205No Hit
TGAACAGCCCAGGGTTTTGATCTAAAATG20.3205128205128205No Hit
TCAACCTTACGACTCATGGTGAACAGCCCA20.3205128205128205No Hit
TCCTTCCCTTGTTCTGTGATGATCCGTGAC20.3205128205128205No Hit
TCACGAGATTTGAAGAGCTCATACAGTC20.3205128205128205No Hit
ACCAATTCCCAGACAAGTAGTACAATGGTT20.3205128205128205No Hit
TACAAACCCCTTGGAGACCTTAAAACACG20.3205128205128205No Hit
AAGGATCCCGGCCTAAGCTCGCATAGCTTCA20.3205128205128205No Hit
TTAACATTGGACTCACGAGATTTGAAGAGCTCAA20.3205128205128205No Hit
TGAAACAGGATTCCGGCTGGGTACTTC20.3205128205128205No Hit
TGTTCTGTGATGATCCGTGACCTAGGTGAC20.3205128205128205No Hit
CGAACAGACTCGGAAGCGAATACAATAGCCCGT20.3205128205128205No Hit
TTCGTCCAATGTTCTATCTTGTACTGCACT20.3205128205128205No Hit
TATAAAGCATTTGTCCTAGACCAACCCTG20.3205128205128205No Hit
TCAAGAGATGTGACTCTCACGGCCCTCT20.3205128205128205No Hit
GAAGGATCCCGGCCTAAGCTCGCATAGC20.3205128205128205No Hit
TAGGCGAAAGCTTGTACCGTCTTAGAGCT20.3205128205128205No Hit
GGAGACCTTGGAAAAATTGAGTGGGAATAG20.3205128205128205No Hit
TCTCAGACTCCGGCGTTGTCATTCCTTTC20.3205128205128205No Hit
AATTGAGTGGGAATAGGTAAAGGAGAAGAG20.3205128205128205No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGCC20.3205128205128205No Hit
TCGGAATCTGAAGAGATGCCATCTTGCGA20.3205128205128205No Hit
TAACAAGAAAAGATCAGGGGTGGACTAGG20.3205128205128205No Hit
TTTATCTTCGCCTGTGTAGTTAACATTGG20.3205128205128205No Hit
TCCAAGGTGCTCTGTCCGCAGGGAGTG20.3205128205128205No Hit
ACTGAAACAGGATTCCGGCTGGGTACTTC20.3205128205128205No Hit
TGTCCATTTGCAGCCAGTGTACCACCACTC20.3205128205128205No Hit
TTTGCAGCCAGTGTACCACCACTCGTACAGGT20.3205128205128205No Hit
TTAACATTGGACTCACGAGTTTTGAAGAGC20.3205128205128205No Hit
TTATAGACCAAGGATGTCGGTGAGCCACC20.3205128205128205No Hit
TCATTGTAACATTTGTCGTCGAGGGGGAG20.3205128205128205No Hit
TTCTACTCGTTCTTCTTGCCTCTTAATTT20.3205128205128205No Hit
TCATTGTAACATTTGTCGCCGAGGGG10.16025641025641024No Hit
TCCATTTGCAGCCAGTGTACCACCACTCG10.16025641025641024No Hit
TGAGAGAATCAGACCCCTTATAGACCAAGG10.16025641025641024No Hit
TGCAACCGGCAGCTCATTGTAACATTTGTC10.16025641025641024No Hit
TAATTAAGTGGGTTAGGGCTTGTCT10.16025641025641024No Hit
CAGGATTCCGGCTGGGTACTTCTCTGCCT10.16025641025641024No Hit
TGAAACAGGATTCCGGCTGGGTACTTCT10.16025641025641024No Hit
TACCATTCAACCCTAAGTGCTTGTCCTGTC10.16025641025641024No Hit
TGGAGACCTTAAAACACGACGTTTGGGT10.16025641025641024No Hit
TCAACAACTGAAACAGGATTCCCGCTGGGT10.16025641025641024No Hit
TCCAACAGGTTACTCCTTCGTTAAAGGCTC10.16025641025641024No Hit
TCACGGCCCTCTCCCAAGTATGTCCATTT10.16025641025641024No Hit
TTGAAGGATCCCGGCCTAAGCTCGCATAG10.16025641025641024No Hit
TTGGACTCCGGCGTTGTCATTCCTTTCTT10.16025641025641024No Hit
ATACAATAGCCCGTCCCTTTCCTTTTTATAACT10.16025641025641024No Hit
TACCGCCTTAGAGCTGGAAGAGTTCCAGAAA10.16025641025641024No Hit
TGTGACTCTCACGGCCCTCTCCCAAGT10.16025641025641024No Hit
TCTTGTACTGCACTGGCGATATTTTCAAA10.16025641025641024No Hit
TGCAACCGGCAGCTCATTGTAACATTTGT10.16025641025641024No Hit
TCACCACCCAAATCGATTGTGTCGGACTC10.16025641025641024No Hit
TACTGTCACCGCTCCCTCTATGACCTGAAA10.16025641025641024No Hit
TATGTCCATTTGCAGCCAGTGTACCACCACT10.16025641025641024No Hit
TACTGTCACCGCTCCCTCTATGACCTGAG10.16025641025641024No Hit
TAAGCAGGCAAAAGTCCCACGATGGCCAAGT10.16025641025641024No Hit
TACTGGTACGCAAAGTCCTCTGGGCTCATT10.16025641025641024No Hit
TGTGACTCTCACGGCCCTCTCCCAAGTAAAT10.16025641025641024No Hit
TGGGTACTTCTCTGCCAACTGATTGATCA10.16025641025641024No Hit
AAATTGAGTGGGAATAGGTAAAGGAGAA10.16025641025641024No Hit
TCGCACTCGATCTCCTTCCCTTGTTCTGT10.16025641025641024No Hit
TCTGTGATGATCCGTGACCTAGGTGACAT10.16025641025641024No Hit
TAAGAACATCTGTGTTAGGGTTGGGCATC10.16025641025641024No Hit
TAGCCCGTCCCTTTCCTTTTTATAACT10.16025641025641024No Hit
TTGGAAAAATTGAGTGGGAATAGGTAAAGG10.16025641025641024No Hit
ATACATTTATTTAAAAGGACTTGGAGACC10.16025641025641024No Hit
TTGGAAAGTAATTAAGTGGGTTAGGGCT10.16025641025641024No Hit
TGTGACTCTCACGGCCCTCTCCCAAGAAT10.16025641025641024No Hit
TCTTGTACTGCACTGGCGATATTTTCAAAT10.16025641025641024No Hit
ACGAACAGACACGGAAGCGAATACAATAGCCC10.16025641025641024No Hit
TCGGAATCTGAAGAGATGCCATCTTGCGAGA10.16025641025641024No Hit
TTTGAAGGATCCCGGCCTAAGCTCGCATAG10.16025641025641024No Hit
TCACGAGATTTGAAGAGCTCATACAGTCG10.16025641025641024No Hit
TACATTTATTTAAAAGGACTTGGAGA10.16025641025641024No Hit
ATGTCCATTTGCAGCCAGTGTACCACCAC10.16025641025641024No Hit
TGAAACAGGATTCCGGCTGGGTACTTCTCTGCCAA10.16025641025641024No Hit
TTCGATTTCAAGAGATGTGACTCTCACGGC10.16025641025641024No Hit
TTAGGGCTTGTCTCTTCTTCTTGTGGTGG10.16025641025641024No Hit
ACCCCTTGGAGACCTTAAAACACGACGTTTGG10.16025641025641024No Hit
GTAAATATTATACAATCAAAGGGCGAAATT10.16025641025641024No Hit
TCAGGGGTGGACTAGGACAAACACACT10.16025641025641024No Hit
CTTGAAGTTATAAATAGGGACGGGC10.16025641025641024No Hit
TTTTTTTTTCTTGGAGTTATAAATAGGGACGGG10.16025641025641024No Hit
AAGTTATAAATAGGGACGGGCTAAAGACC10.16025641025641024No Hit
TAAGCAGGCAAAAGTCCCACGATGGCCAAG10.16025641025641024No Hit
TAGAAATGAATCTTGCTTTTGGTCAGATT10.16025641025641024No Hit
TGTTCCATCCAAACAGTACGCCCTGTGGAA10.16025641025641024No Hit
ATTTCAAGAGATGTGACTCTCACGGCCCT10.16025641025641024No Hit
AATTGAGTGGGAATAGGTAAAGGAGAAGAGACC10.16025641025641024No Hit
TGTCACCGCTCCCTCTA10.16025641025641024No Hit
CTCTCACGGCCCTCTCCCAAGTATGTCCAT10.16025641025641024No Hit
AGGATCCCGGCCTAAGCTCGCATAGCTTC10.16025641025641024No Hit
AATTGAGTGGGAATAGGTAAAGGAGAAG10.16025641025641024No Hit
TCTTGTACTGCACTGGCGATATTTTCAA10.16025641025641024No Hit
CCTCTGGGCTCATTTTGGCTAAAGTCAACA10.16025641025641024No Hit
TAACATTTGTCGTCGAGGGGGAGAGCG10.16025641025641024No Hit
AACTGAAACAGGATTCCGGCTGGGTACTTC10.16025641025641024No Hit
TAAGAACATCTGTTTTAGGGTTGGGCATC10.16025641025641024No Hit
TCACTTGACTGGAGAGGTTAAAACCAATT10.16025641025641024No Hit
TACGCAAAGTCCTCTGGGCTCATTTTGGCT10.16025641025641024No Hit
TGTAACATTTGTCGTCGAGGGGGAGAGCGC10.16025641025641024No Hit
TGTAACATTTGTCGTCGAGGGGGAGAGCGG10.16025641025641024No Hit
TTCAAGAGATGTGACTCTCACGGCCCT10.16025641025641024No Hit
TCACCGCGAACTACAGCATTGAATCCAGA10.16025641025641024No Hit
TCCATCCAAACAGTACGCCCTGTGGAAT10.16025641025641024No Hit
TTTTGAAGGATCCCGGCCTAAGCTCGCAA10.16025641025641024No Hit
TCACGAGATTTGAAGAGCTCATACAGTCGAT10.16025641025641024No Hit
TGCTATCTGGATTTCACGCAGCGTTGCCAA10.16025641025641024No Hit
TCCTAGACCAACCCTGATCTTTTCCTGCT10.16025641025641024No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGT10.16025641025641024No Hit
AAGGACCTTGCTAACTATTGGACTACCATCA10.16025641025641024No Hit
TAAAGCATTTGTCCTAGACCAACCCTGATC10.16025641025641024No Hit
TCACGAGATTTGAAGAGCTCATACAGTCGA10.16025641025641024No Hit
TTGGAAAGTAATTAAGTGGGTTAGGGC10.16025641025641024No Hit
TCTGGATTTCACGCAGCGTTGCCAACCTGTC10.16025641025641024No Hit
TCACCGCTCCCTCTATGACCTGAAATTGA10.16025641025641024No Hit
TTTGTCGTCGAGGGGGAGAGCGTCTACA10.16025641025641024No Hit
TAGGCAATCTCTACCATTCAACCCTGGTT10.16025641025641024No Hit
AAGGATCCCGGCCTAAGCTCGCATAGCTTCAA10.16025641025641024No Hit
TGAAACAGGATTCCGGCTGGGTACT10.16025641025641024No Hit
TTGAAGGATCCCGGCCTAAGCTCGCA10.16025641025641024No Hit
TGGGAAGTGTTGGGCTCCAAAATACAAACC10.16025641025641024No Hit
TCCGGCGTTGTCATTCCTTTCTTTGCTAGA10.16025641025641024No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGCTT10.16025641025641024No Hit
TTGTAACATTTGTCGTCGAGGGGGAGAG10.16025641025641024No Hit
TTTAACAGGAAAAGATCAGGGGTGGACTAG10.16025641025641024No Hit
TACATTTATTTAAAAGGACTTGGAGACC10.16025641025641024No Hit
CAGGATTCCCGCTGGGTACTTCTCTGCCAAC10.16025641025641024No Hit
TACGAATCTGAAGAGATGCCATCTTGCGA10.16025641025641024No Hit
TTTGAAGGATCCCGGCCTAAGCTCGCAT10.16025641025641024No Hit
GAAGTTATAAATAGGGACGGGCTAAAAAC10.16025641025641024No Hit
TGAAGGATCCCGGCCTAAGCTCGCATAGCTTCTA10.16025641025641024No Hit
TGAAGGATCCCGGCCTAAGCTCGCAA10.16025641025641024No Hit
GAGACCTTGGAAAAATTGAGTGGGAATAGG10.16025641025641024No Hit
GTAAATATTATACAATCAAAGGGCGA10.16025641025641024No Hit
TTGAAGGATCCCGGCCTAAGCTCGCATAGCA10.16025641025641024No Hit
TAGGACAAACACACTATATTTATGTAGGT10.16025641025641024No Hit
TCCCGGCCTAAGCTCGCATAGCTTCTTC10.16025641025641024No Hit
TGTGTTAGGGTTGGGCATCATACAA10.16025641025641024No Hit
TCTCACGGCCCTCTCCCAAGTATGTCCATT10.16025641025641024No Hit
TCCCGCTGGGTACTTCTCTGCCAACTGATT10.16025641025641024No Hit
TATCTGAATACAGCACAAATACAAAAAGAC10.16025641025641024No Hit
TCCAAGGTGCTCTGTCCGCAGGGAGTGAA10.16025641025641024No Hit
TTCAAGAGATGTGACTCTCACGGCCCTCT10.16025641025641024No Hit
TCGTACGAGGCTGTTCAAATAGTATTAGGA10.16025641025641024No Hit
AACAGGTTTTGAAGGATCCCGGCCTAAGCTCGCA10.16025641025641024No Hit
TTATAAATAGGGACGGGCTAAAAAGCGGG10.16025641025641024No Hit
GTAAATATTATACAGTCAAAGGGCGAAATT10.16025641025641024No Hit
TGATCAGTTGCATGGTTTTCGTTGTCTAT10.16025641025641024No Hit
CCTTCCCTTGTTCTGTGATGATCCGTGAC10.16025641025641024No Hit
CGGGCTAAAAAGCGGGATCCCGCATTTGC10.16025641025641024No Hit
TGAAGGATCCCGGCCAAAGCTCGCATAG10.16025641025641024No Hit
TCCAACAGGTTACTCCTTCGTTAAAGGCT10.16025641025641024No Hit
TCCAACAGGTTACTCCTTCGTTAAAGGCTT10.16025641025641024No Hit
TTGGAGACCTTAAAACACGACGTTTGAAC10.16025641025641024No Hit
TCAACCCTAAGTGCTTGTCCTGTCCCCAACT10.16025641025641024No Hit
TTCGAATGCAACCGGCAGCTCATTGTAAC10.16025641025641024No Hit
TTGGAGACCTTGGAAAAATTGAGTGGGA10.16025641025641024No Hit
CTTGGAGACCTTAAAACACGACGTTTGAAC10.16025641025641024No Hit
TCATTCCTTTCTTTGCTAGACATTTTGAA10.16025641025641024No Hit
TTCCATCCAAACAGTACGCCCTGTGGAATT10.16025641025641024No Hit
CCTGTCACAAAACAAAAGTCGTACGAGGCT10.16025641025641024No Hit
TCTACCATTCAACCCTAAGTGCTTGTCCTGT10.16025641025641024No Hit
TAACATTGGACTCACGAGATTTGAAGAGC10.16025641025641024No Hit
TGAAGGATCACGGCCTAAGCTCGCATAGC10.16025641025641024No Hit
ACCATTCAACCCTAAGTGCTTGTCCTGT10.16025641025641024No Hit
TTTTGAAGGATCCCGGCCTAAGCTCGCAT10.16025641025641024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)