FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.12121.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.12121.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length17-35
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACAAGAATAAGAGACAAGCCT3542.68292682926829No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCACC56.097560975609756No Hit
TACTAACACAAGAATAAGAGACAAGCCTCCAC44.878048780487805No Hit
GAAAGGGACGGGCTATTGTATTCGCTTCC33.6585365853658534No Hit
TTTTTAGCCCGTCCCTATTTATAACTTCAAGAAA22.4390243902439024No Hit
AGGAAAGGGACGGGCTA22.4390243902439024No Hit
TGACTCCACCACAAGAAGAAGAGACAAGC22.4390243902439024No Hit
GAAAGGGACGGGCTATTGTATTCGCT22.4390243902439024No Hit
TACTAACACAAGAATAAGAGACAAGCC22.4390243902439024No Hit
AAAGAAAGGAATGACAACGCCGGCGCC11.2195121951219512No Hit
AATTTCGCCCTTTGATTGTATACTATTTACT11.2195121951219512No Hit
TTCGCCCTTTGATTGTATAATATTTGCTGT11.2195121951219512No Hit
TTTAGCCCGTCCCTATTTATAACTA11.2195121951219512No Hit
ATGGTAGAGCTTGCCTAAGGCAATAATTTCGCCCT11.2195121951219512No Hit
TTTCGCCCTTTGATTGTATAATATTTACTGT11.2195121951219512No Hit
TTAGCCCGTCCCTATTTATAACTTCAAGG11.2195121951219512No Hit
TTATAAAAAGGTAAGGGACGGGCTATTGT11.2195121951219512No Hit
TACTAACACAAGAATAAGAGACAAGCCTC11.2195121951219512No Hit
TTTCGCCCTTTGATTGTATAATATTTACTGTC11.2195121951219512No Hit
GACTTTTTGCCTGCTTAGGACCAATG11.2195121951219512No Hit
ATAAAAAGGAAAGGGACGGGCTATTGTATTCGCTT11.2195121951219512No Hit
AAAAGGAAAGGGACGGGCTATTGTATTC11.2195121951219512No Hit
TACTAACACAAGAATAAGAGACAATCCT11.2195121951219512No Hit
GTTATAAAAAGGAAAGGGACGGGCTATTGTA11.2195121951219512No Hit
TGACTCCACCACAAGAAGAAGAGACAAGCCCT11.2195121951219512No Hit
AACACAAGAATAAGAGACAAGCCTCCACC11.2195121951219512No Hit
TAAAAAGGAAAGGGACGGGCTATTGTATT11.2195121951219512No Hit
TATACTAACACAAGAATAAGAGACAAGCCTCCACC11.2195121951219512No Hit
TACTAACACAAGAATAAGAGACAAGCATCCACC11.2195121951219512No Hit
AAAAGGACGGGCTATTGTATTCGCTT11.2195121951219512No Hit
TTCGCCCTTTGATTGTATAATATTTACTGT11.2195121951219512No Hit
TTGTATTCGCTTCCGTGTCTGTTCGTTC11.2195121951219512No Hit
AAAAGTTATAAAAAGGAAAGGGACGGGCT11.2195121951219512No Hit
AATTGTATGATGCCCAACCCTAACAGAA11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)