FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.12682.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.12682.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length15-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTGGGCCCCGAAAACAGCCAGTCGCAG910.227272727272728No Hit
TAGGTGGGCCCCGAAAACAGCCAGTCACAG66.8181818181818175No Hit
TGCAGTGATCATCGCGCCATGGGAGCTG33.4090909090909087No Hit
TCACAGGATAGAACATTTTACATGCGAAAAA33.4090909090909087No Hit
TCGAACCCGGGACTCCCGCGTGCAAGGCGAA33.4090909090909087No Hit
TCATAGCTACCTATTTTCCTCACGAGGCT33.4090909090909087No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAG33.4090909090909087No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAGA22.272727272727273No Hit
TTTTACATGCGAAAAATCTTCGACCCCGCC22.272727272727273No Hit
TTTACCCACAATGCTGACACCGAGTCGC22.272727272727273No Hit
CACGGGATAGAACATTTTACATGCGAAAAATC22.272727272727273No Hit
TACATGCGAAAAATCTTCGACCCCGCCGGGAA22.272727272727273No Hit
TTTTCCTCACGAGGCTAGGTGGGCCCCGAAA22.272727272727273No Hit
TCACAGGATAGAACATTTTACATGCGAAAA22.272727272727273No Hit
TTTTACCCACAATGCTGACACCGAGTCGC22.272727272727273No Hit
TATTTTCCTCACGAGGCTAGGTGGGCCCC22.272727272727273No Hit
TTTACCCACAAAGCTGACACCGAGTCGCTATGC11.1363636363636365No Hit
ATGCTGACACCGAGTCGCTATGCGCATT11.1363636363636365No Hit
ATTTTCCTCACGAGGCTAGGTGGG11.1363636363636365No Hit
ATGTTTTGGAGACGTTGCACTTGGCTT11.1363636363636365No Hit
CGTGGAGGACTTCCA11.1363636363636365No Hit
TACATGCGAAAAATCTTCGACCCCGCC11.1363636363636365No Hit
AACATTTTACATGCGAAAAATCTTCGCCCCCGCCG11.1363636363636365No Hit
TGCAGTGATCATCGCGCCATGGGAGCT11.1363636363636365No Hit
TCTTCGCCCCCGCCGGGAATCGAACCCGA11.1363636363636365No Hit
TTTTACCCACAATGCTGATACCGAGT11.1363636363636365No Hit
ATTTTACCCACAAAGCTGACACCGAGTCGC11.1363636363636365No Hit
TGGGCCCCGAAAACAGCCAGTCACAGGATAGAA11.1363636363636365No Hit
CTCCCGCGTGCAAGG11.1363636363636365No Hit
TTACCCACAATGCTGACACCGAGTCGCTATGCGC11.1363636363636365No Hit
TTTTCCTCACGAGGCTAGGTGGGCC11.1363636363636365No Hit
AAATTTTACCCACAATGCTCACACCGAGTCGC11.1363636363636365No Hit
TTTTACCTACAATGCTGACACCGAGTCGCT11.1363636363636365No Hit
ATGCAGTGATCATCGCGCCATGGGAGCTG11.1363636363636365No Hit
AATCGAACTCGAGACTCCCGCGTGCAAGGC11.1363636363636365No Hit
TAGCTACCTATTTTCCTCACGAGG11.1363636363636365No Hit
TCACAGGATAGAACATTTTACATGCGAAAAAT11.1363636363636365No Hit
TCGAACTCGGGACTCCCGCGTGCAAGGCGA11.1363636363636365No Hit
TAAATTTTACCCACAATGCTGACACCGAGTCGC11.1363636363636365No Hit
TCCCGCGTGCAAGGCGAATGCAGTGATC11.1363636363636365No Hit
TCATAGCTACCTATTTTCCTCACGAGGATA11.1363636363636365No Hit
TTATTTCATAGCTACCTATTTTCCTCACGA11.1363636363636365No Hit
TACCCACAATGCTGACACCAAGTCGCTATGCGCA11.1363636363636365No Hit
TTTTACCCACAAAGCTGACACCGAGTCGC11.1363636363636365No Hit
TTTTACCCACAAAGCTGACACCGAGTC11.1363636363636365No Hit
CCTATTTTCCTCACGAGGCTAGGTGGGCC11.1363636363636365No Hit
GAATCGAACCCGGGACTCCCGCGTGCAAGGCGAA11.1363636363636365No Hit
TTAAATTTTACCCACAATGCTGACACCGAG11.1363636363636365No Hit
TCGAACCCGGGACTCCCGCGTGCAAGGCGA11.1363636363636365No Hit
ACATTTTACATGCGAAAAATCTTCGACCCC11.1363636363636365No Hit
TTTTACATGCAAAAAATCTTCGCCCCCGCC11.1363636363636365No Hit
TTTACCCACAAAGCTGACACCGAGTCGCTAT11.1363636363636365No Hit
AAAATCTTCGCCCCCGCCGGAAATCGAAC11.1363636363636365No Hit
TCATAGCTACCTATTTTCCTCACGAGGAT11.1363636363636365No Hit
TTCCTCACGAGGATAGGTGGGCCCCGAAA11.1363636363636365No Hit
TTAAATTTTACCCACAATGCTGACACCGAGTCGC11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)