FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.16463.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.16463.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571
Sequences flagged as poor quality0
Sequence length15-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTTAGTACACCACACCGGGAGACGAC559.632224168126093No Hit
TAGGTTCTTGAGAATATCGGCACGGAGC437.530647985989491No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGC264.553415061295972No Hit
TACGCCCTTCTCTATACTGCCGAAACTGGT132.276707530647986No Hit
TTCTTGAGAATATCGGCACGGAGCTCGC91.5761821366024518No Hit
TGTAACATTATTTGCCCGAGCGCCACCACT81.4010507880910683No Hit
TAGGTTCTTGAGAATATCGGCACGGAGCT81.4010507880910683No Hit
TAGAACAAGGGCCTGTAATCTACACTAGG71.2259194395796849No Hit
TTAACGGTTTGGTCACCACACTTGAATGCC71.2259194395796849No Hit
AACATTATTTGCCCGAGCGCCACCACTGC71.2259194395796849No Hit
TGTAATCATAGAACCTGAACCACGGTAAACC61.0507880910683012No Hit
TGCATACGCCCTTCTCTATACTGCCGAAAC61.0507880910683012No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACTG61.0507880910683012No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACT61.0507880910683012No Hit
ATTTTGCCCGAGCGCCACCACTGCTGGTCA61.0507880910683012No Hit
TAATATAAAATGGAGGGTTAATTAGAGT50.8756567425569177No Hit
TGAGATTGGTTCAACTCATATAGGCAGGCGGG50.8756567425569177No Hit
TTAGTACACCACACCGGGAGACGACATGTC50.8756567425569177No Hit
TACTGCATTGAAACCGGAGTCCGAAAAAT50.8756567425569177No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGCA50.8756567425569177No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGCC50.8756567425569177No Hit
TGAGAAAATCGGCACGGAGCTCGCATCGTT50.8756567425569177No Hit
TTAGTACACCACACCGGGAGACGACATGT40.7005253940455342No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGC40.7005253940455342No Hit
TGCCCATGTTGCCTCCCGATTAGTACTC40.7005253940455342No Hit
TATTTGCCCGAGCGCCACCACTGCTGGTC40.7005253940455342No Hit
GACTATCAGTTAGTACACCACACCGGGA40.7005253940455342No Hit
TCGATACAATCTCTCAACCTGTTGCTTT40.7005253940455342No Hit
TTGGCCGCTAGAGTGCCACCACTGGTGCATAAA40.7005253940455342No Hit
TTTGTAACATTATTTGCCCGAGCGCCACC40.7005253940455342No Hit
TATGACAGACCAGAAACATTGCCCTAACTA40.7005253940455342No Hit
TATCAGTTAGTACACCACACCGGGAGAC40.7005253940455342No Hit
TCGTCACCTCCGAGATCTAGTAGGTCAGAT40.7005253940455342No Hit
TCACCTCCGAGATCTAGTAGGTCAGATTC40.7005253940455342No Hit
TATGACAGACCAGAAACATTGCCCAAACTA30.5253940455341506No Hit
TTATTTGCCCGAGCGCCACCACTGCTGGT30.5253940455341506No Hit
TATCACATATCCAAAATTCAGCTTTCCCCT30.5253940455341506No Hit
AAGAGAAACAAATCGGCGGCCGTAAT30.5253940455341506No Hit
TCAATAGGTTCTTGAGAATATCGGCACGG30.5253940455341506No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCAATT30.5253940455341506No Hit
TATGGGTATTTGGTCAAGCAGGGCAGGA30.5253940455341506No Hit
TCTTGAGAATATCGGCACGGAGCTCGCATC30.5253940455341506No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGG30.5253940455341506No Hit
TGAATAGAAAGTGTGGAAACTCCCCCCCGCCT30.5253940455341506No Hit
TCATAGAACCTGAACCACGGTAAACCAATG30.5253940455341506No Hit
TTCTTGAGAATATCGGCACGGAGCTCGCA30.5253940455341506No Hit
TATGACAGACCAGAAACATTGCCCAAACTAT30.5253940455341506No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGC30.5253940455341506No Hit
TGTGTACCACCTTCACAAGCTTGTCTCCTT30.5253940455341506No Hit
TAGGTTCTTGAGAATATCGGCACGGAG30.5253940455341506No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGCT30.5253940455341506No Hit
TCAGATTCGTAATCCGCCTCACCTGTCCCT30.5253940455341506No Hit
TCGTAATCCGCCTCACCTGTCCCTAACTG30.5253940455341506No Hit
ACTCATATAGGCAGGAGGGGGGTGGAA20.3502626970227671No Hit
TCCGCCTCACCTGTCCCTAAC20.3502626970227671No Hit
TATACTGCATTGAAACCGGAGTCCGAAAAAT20.3502626970227671No Hit
TTATCCAGAGTTTGCCCATGTTGCCTCCCG20.3502626970227671No Hit
TTGGTTCAACTCATATAGGCAGGCGGGG20.3502626970227671No Hit
TATGTGTACCACCTTCACAAGCTTGTCTCCT20.3502626970227671No Hit
TTATTTGCCCGAGCGCCACCACTGCTGGTC20.3502626970227671No Hit
TCCGCCTCACCTGTCCCTAACTGGAATTCGA20.3502626970227671No Hit
TCCAGAAGACGAAACCTTTGGCCACCTT20.3502626970227671No Hit
ATAGAAAGTGTGGAAACTCCCCCCCCGCCT20.3502626970227671No Hit
ATGAGACTGGTTCAACTCATATAGGCAGGCGGGG20.3502626970227671No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCGATT20.3502626970227671No Hit
TATGTAATCTTGGGGGGTATTATTTAAGAA20.3502626970227671No Hit
TATGTAATCTTGGGGGGTATTATTTAAG20.3502626970227671No Hit
TGTGGTCACAAGTGTCCTGCATTCCTCTCTTC20.3502626970227671No Hit
ATTGAATAGAAAGTGTGGAAACTCCCCCCCGCCT20.3502626970227671No Hit
TGAGATTGGTTCAACTCATATAGGCAGGAGG20.3502626970227671No Hit
ACATTATTTGCCCGAGCGCCACCACTGCT20.3502626970227671No Hit
TGCCCATGTTGCCTCCCGATTAGTACTCG20.3502626970227671No Hit
TAATCATAGAACCTGAACCACGGTAAACCA20.3502626970227671No Hit
GCGTTCACTTTGTAACATTATTTGCCCGAG20.3502626970227671No Hit
TTGGTCGTCACCTCCGAGATCTAGTAGGT20.3502626970227671No Hit
TTTCCGGGCTTTACCATACCAATAAAACCA20.3502626970227671No Hit
AGACTGGTTCAACTCATATAGGCAGGCGGGGG20.3502626970227671No Hit
TGAGAATATCGGCACGGAGCTCGCATCGTT20.3502626970227671No Hit
AATTTGCCCGAGCGCCACCTCTGCTGGTCA20.3502626970227671No Hit
TCCGCCTCACCTGTCCCTAACTGGAATTCGAT20.3502626970227671No Hit
TTATAAACAAGCCGGGGTGCTCAGTCTT20.3502626970227671No Hit
TGAGATTGGTTCAACTCATATAGGCAGGCGGGG20.3502626970227671No Hit
GTTCTTGAGAAAATCGGCACGGAGCTCGCA20.3502626970227671No Hit
TTTCGGTATGTGGAGTAGTGATATAGCC20.3502626970227671No Hit
TTTTGCCCGAGCGCCACCACTGCTGGTC20.3502626970227671No Hit
TTTGCCCATGTTGCCTCCCGATTAGTACTCG20.3502626970227671No Hit
TATGACAGACCAGAAACATTGCCCAAACT20.3502626970227671No Hit
ATGAGATTGGTTCAACTCATATAGGCAGGCGGAGG20.3502626970227671No Hit
ATAGGTTCTTGAGAAAATCGGCACGGAGC20.3502626970227671No Hit
TTGAGAATATCGGCACGGAGCTCGCATC20.3502626970227671No Hit
TCAACTCATATAGGCAGGAGGGGGGTGGA20.3502626970227671No Hit
TCAAGCAGGGCAGGATTAGATAGGAGCC20.3502626970227671No Hit
AACGGTTTGGTCACCACACTTGAATGCC20.3502626970227671No Hit
TGCATACGCCCTTCTCTATACTGCCGAAACT20.3502626970227671No Hit
TAGTACACCACACCGGGAGACGACATGTC20.3502626970227671No Hit
TGTAACATTATTTGCCCGAGCGCCACCAC20.3502626970227671No Hit
ATGAGACTGGTTCAACTCATATAGGCAGGCGGGGG20.3502626970227671No Hit
AGGTTCTTGAGAATATCGGCACGGAGC20.3502626970227671No Hit
AGGAGAAACAAACCGGCGGCCG20.3502626970227671No Hit
TTAACGGTTTGGTCACCACACTTGAATGCCT20.3502626970227671No Hit
AAGAGAAACAAACCGGCGGCCGTAAA10.17513134851138354No Hit
AGATTGGTTCAACTCATATAGGCAGGAGGGGGGT10.17513134851138354No Hit
CATGAGATGGGTTCAACTCATATAGGCAGGCGGGG10.17513134851138354No Hit
AATTCAGCTTTCCCCTAATTATTTGCGC10.17513134851138354No Hit
TTGGCCCATGTTGCCTCCCGATTAGTACTC10.17513134851138354No Hit
GCATTGGTCGTCACCTCCGAGATCTAGTA10.17513134851138354No Hit
TCGTAATCCGCCTCACCTGTCCCTAAC10.17513134851138354No Hit
TTCACTTTGTAACATTATTTGCCCGAGCGCC10.17513134851138354No Hit
TTGGTCGTCACCTCCGAGATCTAGTAGG10.17513134851138354No Hit
ATTGAATAGAAAGTGTGGAAACTCCCCCCCGC10.17513134851138354No Hit
TCCGCCTCACCTGTCCCTAACTGGAATTCG10.17513134851138354No Hit
TCACTTTGTAACATTATTTGCCCGAGCGC10.17513134851138354No Hit
TATAGGCAGGCGGGGG10.17513134851138354No Hit
TTGGTTCAACTCATATAGGCAGGAGGGGGGT10.17513134851138354No Hit
TCTCTCAACCTGTTGCTTTGTATGTCTCAT10.17513134851138354No Hit
GAATAGAAAGTGTGGAAACTCCCCCCCGCCT10.17513134851138354No Hit
AGATTGGTTCAACTCATATGGGCAGGCGGGG10.17513134851138354No Hit
ATTGAATAGAAAGTGTGGAAACTCCCCCCCCCGC10.17513134851138354No Hit
TGCCACCACTGGTGCATACGCCCTTCTCTA10.17513134851138354No Hit
CGTATTGAATAGAAAGTGTGGAAACTCCCCCCCC10.17513134851138354No Hit
TCATATAGGCAGGCGGGGGGTGG10.17513134851138354No Hit
TTTGCCCGAGCGCCACCTCTGCTGGTCA10.17513134851138354No Hit
TCTTACTGCCCTTTCCCAGGTGTGGCCATT10.17513134851138354No Hit
CGTGGTCGGTATAATCTCCATGTATGCTCCAGCA10.17513134851138354No Hit
ACCACCTTCACAAGCTTGTCTCCTTCTGG10.17513134851138354No Hit
TGACAGACCAGAAACATTGCCCTAACTATT10.17513134851138354No Hit
TAATGTTATAAACAAGCCGGGGTGCTCA10.17513134851138354No Hit
CAGTTAGTACACCACACCGGGAGACGAC10.17513134851138354No Hit
TCAACTCATATAGGCAGGCGGGGGTGT10.17513134851138354No Hit
TCGGTATAATCTCCATGTATGCTCCAGCATCT10.17513134851138354No Hit
TTGCCCATGTTGCCTCCCAATTAGTACTC10.17513134851138354No Hit
TCCAATTTTACATCAGAGTGTGGTAGATG10.17513134851138354No Hit
TTTGTAACATTATTTGCCCGAGCGCCACCAC10.17513134851138354No Hit
AGATTGGTTCAACTCATATAGGCAGGAGGG10.17513134851138354No Hit
AGATTGGTTCAACTCATATAGGCTGGCGGGGGGT10.17513134851138354No Hit
TTGGTCACCACACTTGAATGCCATAACTGCC10.17513134851138354No Hit
GTATTGAATAGAAAGTGTGGAAACTCCCCCCCGC10.17513134851138354No Hit
TGCCACCACTGGTGCATACGCCCTTC10.17513134851138354No Hit
TCTTGAGAATATCGGCACGGAGCTCGCA10.17513134851138354No Hit
TGTACCACCTTCACAAGCTTGTCTCCTTCT10.17513134851138354No Hit
CATTATTTGCCCGAGCGCCACCACTGCT10.17513134851138354No Hit
TTTTGCCCGAGCGCCACCACTGCTGGTCA10.17513134851138354No Hit
TAGAACAAGGGCCTGTAATCTAGACTAGGAG10.17513134851138354No Hit
TGGGTTGTATATAATATAAAATGGAGGGT10.17513134851138354No Hit
ATTGGTTCAACTCATATAGGCAGGCGGGAGGTG10.17513134851138354No Hit
GATTGGTTCAACTCATATAGGCAGGCGGT10.17513134851138354No Hit
TGAAGGTTTATGTAATCTTGGGGGGTATT10.17513134851138354No Hit
TCCAGAAGACGAAACCTTTGGCCACCTTA10.17513134851138354No Hit
TGTGGTCACAAGTGTCCTGCATTCCTCTC10.17513134851138354No Hit
TTGTAACATTATTTGCCCGAGCGCCACCAC10.17513134851138354No Hit
TGAAACCGGAGTCCGAAAAATATTGATACG10.17513134851138354No Hit
CTATCAGTTAGTACACCACACCGGGAGACGAC10.17513134851138354No Hit
TCAGATTCGTAATCCGCCTCACCTGTCCC10.17513134851138354No Hit
CGTATTGAATAGAAAGTGTGGAAACTCCCCCCCGC10.17513134851138354No Hit
TTTGCCCATGTTGCCTCCCGATTAGTACTCGAA10.17513134851138354No Hit
TGTGGAGTAGTGATATAGCCGTCTGATTA10.17513134851138354No Hit
ATAGGCAGGCGGGGGGTGGAAAAAAC10.17513134851138354No Hit
AGGGCAGGATTTGATAGGAGCCAAAAGAA10.17513134851138354No Hit
TCCAAAATTCAGCTTTCCCCTAATTATTTGC10.17513134851138354No Hit
AGGGGCATTGTTGCATTTCGGTATGTGG10.17513134851138354No Hit
CTGGATTCAACTCATATAGGCAGGCGGGGGGTGG10.17513134851138354No Hit
TCTTGAGAAAATCGGCACGGAGCTCGCATC10.17513134851138354No Hit
TATTGAATAGAAAGTGTGGAAACTCCCCCC10.17513134851138354No Hit
TGATGTATGACAATATATTCAAGGAGGGA10.17513134851138354No Hit
TGACGTTTGTGTGAGACGGCATTGGTC10.17513134851138354No Hit
AGGTTCTTGAGAAAATCGGCACGGAGC10.17513134851138354No Hit
CAACTCATATAGGCAGGCGGGG10.17513134851138354No Hit
CTTTGGCCACCTTAAAACATGACGTTTGT10.17513134851138354No Hit
ATTGGTTCAATTCATATAGGCAGGCGGGGG10.17513134851138354No Hit
TACCACCTTCACAAGCTTGTCTCCTTCTGG10.17513134851138354No Hit
TCTTGAGAAAATCGGCACGGAGCTCGC10.17513134851138354No Hit
GTATTGAATAGAAAGTGTGGAAACTCCCCCCCCGC10.17513134851138354No Hit
TAACGGTTTGGTCACCACACTTGAATGCC10.17513134851138354No Hit
TTTGCCCGAGCGCCACCTCTGCTGGTCAG10.17513134851138354No Hit
TGGTCGTCACCTCCGAGATCTAGTAGGTC10.17513134851138354No Hit
TGAATAGAAAGTGTGGAAACTCCCCCCCT10.17513134851138354No Hit
ACCCAGGGCTCGGTTAGGTA10.17513134851138354No Hit
TATAGAGAGTGCTGT10.17513134851138354No Hit
AAGGTTTATGTAATCTTGGGGGG10.17513134851138354No Hit
GAGAAACAAACCGGCGGCCGT10.17513134851138354No Hit
TGATAGGAGCCAAAAGAAATGGGTTGTAT10.17513134851138354No Hit
CAACTCATATAGGCAGGCGGGGG10.17513134851138354No Hit
ATAACTCGAGCGTTCACTTTGTAACATTA10.17513134851138354No Hit
TATGGGTATTTGGTCAAGCAGGGCAGGAT10.17513134851138354No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGGA10.17513134851138354No Hit
AATTCAGCTTTCCCCTAATTATTTGCGCAT10.17513134851138354No Hit
TACAATCTCTCAACCTGTTGCTTTGTATGT10.17513134851138354No Hit
ATGGGTATTTGGTCAAGCAGGGCAGGAT10.17513134851138354No Hit
TAACGGTTTGGTCACCACACTTGAATGCCT10.17513134851138354No Hit
TCACATATCCAAAATTCAGCTTTCCCCTAAT10.17513134851138354No Hit
TCAAGCAGGGCAGGATTAGATAGGAGCCAAA10.17513134851138354No Hit
TAGATGATATTCTCGTGTGGTCACAAGT10.17513134851138354No Hit
TCTTGAGAAAATCGGCACGGAGCTCGCA10.17513134851138354No Hit
TATGTAATCTTGGGGGGTATTATTTAAGA10.17513134851138354No Hit
GAGATTGGTTCAACTCATATAGGCAGGCGGGG10.17513134851138354No Hit
TCCGGGCTTTACCATACCAATAAAACCA10.17513134851138354No Hit
TTCTTGAGAAAATCGGCACGGAGCTC10.17513134851138354No Hit
CAAACTCATATAGGCAGGCGGGGGGTGGAAAT10.17513134851138354No Hit
CTTGAGAAAATCGGCACGGAGCTCGCATC10.17513134851138354No Hit
TCGATTTCTAGGGAAGTAACTCTTACTGCC10.17513134851138354No Hit
TAATCCGCCTCACCTGTCCCTAA10.17513134851138354No Hit
TGTGTACCACCTTCACAAGCTTGTCTCCTTC10.17513134851138354No Hit
TGAGATTGGTTCAACTCATATAGGCAGGAGGGG10.17513134851138354No Hit
TCAAACAATCGATACAATCTCTCAACCTGT10.17513134851138354No Hit
TCACCACACTTGAATGCCATAACTGCTCCAT10.17513134851138354No Hit
TAAGAAACCTGGTGTGTGGGTGGTAGAT10.17513134851138354No Hit
GGGTATTTGGTCAAGCAGGGCAGGAT10.17513134851138354No Hit
CATATAGGCAGGCGGGGGGTGGAAATAACC10.17513134851138354No Hit
GCGTAAAGTACTCGCCAAGAGAAACAAATCGGCG10.17513134851138354No Hit
TTTTCAAACAATCGATACAATCTCTCAACCT10.17513134851138354No Hit
TTTTACTACTAGCGCTCCCA10.17513134851138354No Hit
TATTTGCCCGAGCGCCACCACTGCTGGTCACT10.17513134851138354No Hit
TCGGTATGTGGAGTAGTGATATAGCCGTCT10.17513134851138354No Hit
TTATTTGCCCGAGCGCCACCACTGCT10.17513134851138354No Hit
AGAAAGTGTGGAAACTCCCCCCCGCCT10.17513134851138354No Hit
TCGGTATAATCTCCATGTATGCTCCAGC10.17513134851138354No Hit
CTATCAGTTAGTACACCACACCGGGAGAC10.17513134851138354No Hit
TATTATCCAGAGTTTGCCCATGTTGCCTCC10.17513134851138354No Hit
AGGCAGGCGGGGGGTGGAAAAAC10.17513134851138354No Hit
AATAGGTTCTTGAGAAAATCGGCACGG10.17513134851138354No Hit
TTTGGCCACCTTAAAACATGACGTTTGTGT10.17513134851138354No Hit
TAGCCGTCTGATTAACGGTTTGGTCACCACA10.17513134851138354No Hit
TAACATTATTTGCCCGAGCGCCACCACTGC10.17513134851138354No Hit
TTCGATTTCTAGGGAAGTAACTCTTACTGCC10.17513134851138354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)