FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.16656.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.16656.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065
Sequences flagged as poor quality0
Sequence length21-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTCAGACTAGAGACAACCCGAGAGC60329.200968523002423No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAGC21210.26634382566586No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAG1376.634382566585957No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGA683.2929782082324457No Hit
TCAGACTAGAGACAACCCGAGAGCTCAAT582.8087167070217918No Hit
TCAGTTAGTACACCACACCGGGAGACGAC552.663438256658596No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAG522.5181598062953996No Hit
TCCTGTCAGACTAGAGACAACCCGAGAG462.2276029055690074No Hit
TAGGTTCTTGAGAATATCGGCACGGAGC432.082324455205811No Hit
TTGGAGGAAAACTACGGCTCAACCCGGGC381.8401937046004841No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGA311.5012106537530265No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATA271.3075060532687652No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGC261.2590799031476998No Hit
TTTGGAGGAAAACTACGGCTCAACCCGGGC251.2106537530266344No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAG231.1138014527845037No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATAT231.1138014527845037No Hit
CCTGTCAGACTAGAGACAACCCGAGAGC150.7263922518159807No Hit
TACGCCCTTCTCTATACTGCCGAAACTGGT130.6295399515738499No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAGC120.5811138014527845No Hit
TCCAAGGACCCATCTCTCACGTGATTAGCA110.5326876513317191No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGCT110.5326876513317191No Hit
TCCCCTGGCTGTACCAATTGCCTGACAAAT110.5326876513317191No Hit
CTTTTCCTGTCAGACTAGAGACAACCCGAG100.48426150121065376No Hit
TGTCAGACTAGAGACAACCCGAGAGCTCAAT90.4358353510895884No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAA90.4358353510895884No Hit
TCTACCCCACCACAGAGCAAAGAATCCTCCC90.4358353510895884No Hit
TTCTTGAGAATATCGGCACGGAGCTCGC90.4358353510895884No Hit
TCAGACTAGAGACAACCCGAGAGCTCAA90.4358353510895884No Hit
TAGGTTCTTGAGAATATCGGCACGGAGCT80.387409200968523No Hit
TCTTCGTCGAGACGAGAATTCATTATACGC80.387409200968523No Hit
TTAACGGTTTGGTCACCACACTTGAATGCC70.3389830508474576No Hit
TGTAATCATAGAACCTGAACCACGGTAAACC60.29055690072639223No Hit
TGCATACGCCCTTCTCTATACTGCCGAAAC60.29055690072639223No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACTG60.29055690072639223No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACT60.29055690072639223No Hit
TAATATAAAATGGAGGGTTAATTAGAGT50.24213075060532688No Hit
TTAGTACACCACACCGGGAGACGACATGTC50.24213075060532688No Hit
TACTGCATTGAAACCGGAGTCCGAAAAAT50.24213075060532688No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGCA50.24213075060532688No Hit
TTCCTGTCAGACTAGAGACAACCCGAGA50.24213075060532688No Hit
TGTTTGACGCCTTCATTCCCTCTGGCAGC50.24213075060532688No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGCC50.24213075060532688No Hit
TGAGAAAATCGGCACGGAGCTCGCATCGTT50.24213075060532688No Hit
TTTCCTGTCAGACTAGAGACAACCCGAG50.24213075060532688No Hit
TGAAGGAGGTTAGAATCAAAGTGAGCCT50.24213075060532688No Hit
TGAAATCTACCCCACCACAGAGCAAAGAATC50.24213075060532688No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAAT50.24213075060532688No Hit
TTAGTACACCACACCGGGAGACGACATGT40.1937046004842615No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGC40.1937046004842615No Hit
TTGAGAAAATCGGCACGGAGCTCGCATC40.1937046004842615No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGC40.1937046004842615No Hit
TGCCCATGTTGCCTCCCGATTAGTACTC40.1937046004842615No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAGC40.1937046004842615No Hit
GACTATCAGTTAGTACACCACACCGGGA40.1937046004842615No Hit
TCGATACAATCTCTCAACCTGTTGCTTT40.1937046004842615No Hit
TTGGCCGCTAGAGTGCCACCACTGGTGCATAAA40.1937046004842615No Hit
TCTGTCTCTGACAGAATGTTTGACGCCT40.1937046004842615No Hit
TATGACAGACCAGAAACATTGCCCTAACTA40.1937046004842615No Hit
TATCAGTTAGTACACCACACCGGGAGAC40.1937046004842615No Hit
TCGTCACCTCCGAGATCTAGTAGGTCAGAT40.1937046004842615No Hit
TCACCTCCGAGATCTAGTAGGTCAGATTC40.1937046004842615No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAAA40.1937046004842615No Hit
TATGACAGACCAGAAACATTGCCCAAACTA30.14527845036319612No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAG30.14527845036319612No Hit
TGTCAGACTAGAGACAACCCGAGAGCTC30.14527845036319612No Hit
TCAATAGGTTCTTGAGAATATCGGCACGG30.14527845036319612No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCAATT30.14527845036319612No Hit
TATGGGTATTTGGTCAAGCAGGGCAGGA30.14527845036319612No Hit
TCTTGAGAATATCGGCACGGAGCTCGCATC30.14527845036319612No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGG30.14527845036319612No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATAA30.14527845036319612No Hit
TCATAGAACCTGAACCACGGTAAACCAATG30.14527845036319612No Hit
TTCTTGAGAATATCGGCACGGAGCTCGCA30.14527845036319612No Hit
TATGACAGACCAGAAACATTGCCCAAACTAT30.14527845036319612No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGC30.14527845036319612No Hit
TGTGTACCACCTTCACAAGCTTGTCTCCTT30.14527845036319612No Hit
TAGGTTCTTGAGAATATCGGCACGGAG30.14527845036319612No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGCT30.14527845036319612No Hit
TCAGATTCGTAATCCGCCTCACCTGTCCCT30.14527845036319612No Hit
TCATTCCCTCTGGCAGCGCACCTTTTCCTC30.14527845036319612No Hit
TCAGACTAGAGACAACCCGAGAGCTCA30.14527845036319612No Hit
TCGTAATCCGCCTCACCTGTCCCTAACTG30.14527845036319612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)