FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.22483.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.22483.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length27-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGCCTGCCATGGCCGATCTTTTTCTTCT216.666666666666664No Hit
TTTGTTCAAAATATGACTGCGCACGCGAGC18.333333333333332No Hit
TTCCTAGGGATACAGCACTCGCACCTCCT18.333333333333332No Hit
TTTCCTAGGGATACAGCACTCGCACCTCCT18.333333333333332No Hit
CGGCGTCCCAATCGCCCCGTTCCACAG18.333333333333332No Hit
TATGCCTGCCATGGCCGATCTTTTTCTAATT18.333333333333332No Hit
TTATAATGCGCACTTAGAGCCTTTTGCACAA18.333333333333332No Hit
TATGCCTGCCATGGCCGATCTTTTTGTTCT18.333333333333332No Hit
TGCACGTGCTCTGAAAAAGTTGCCGACCCC18.333333333333332No Hit
GTTCAAAATATGACTGCGCACGCGAGCTC18.333333333333332No Hit
TTGTTCAAAATATGACTGCGCACGCGAG18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)