FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.22483.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.22483.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length26-32
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGAAAAGCTTACTCGGCCTGTGCATC3333.0No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAA77.000000000000001No Hit
TCAGGGGTCGGCAACTTTTTCAGAGCGC55.0No Hit
TACATCAGGGGTCGGCAACTTTTTCAGAGC44.0No Hit
TCGGCAACTTTTTCAGAGCGCGTGCGAAAA33.0No Hit
TTCTGCTGCTAAGTCTGCTCGTACTGAAGG33.0No Hit
CAGGCATACATAAGAAGCGCAATGGTTCCACC22.0No Hit
TACATAAGAAGCGCAATGGTTCCACCTAGG22.0No Hit
TTCTACAAATCCTACGGAATCAAGCTCAAC22.0No Hit
TCGTACTGAAGGAACTGGGGAAACTGTG22.0No Hit
TCAAAGAAAAGCTTACTCGGCCTGTGCATCA22.0No Hit
TCGGCTAAGTCTACACCACCATATTTGTA22.0No Hit
GCAAACAAGAAAAAGATCGGCCATGGCAG11.0No Hit
TACCTCAATTAAATCCTTCCACCCAAGATC11.0No Hit
TATAAAAAATCAAACTCCACCTTACCTTT11.0No Hit
TCCCTAGGAAATTCGTCGGCCTCACAAGT11.0No Hit
TCTATTGCAACGACTGCACGCAAACGAGGC11.0No Hit
TGGTCCCAACTGGTTTGTCTGATATGAGT11.0No Hit
TCTATCGGCTAAGTCTACACCACCATATT11.0No Hit
TACAACTGTGGAACGGAGCGATTGGGACGC11.0No Hit
TGAAGGAACTGGGGAAACTGTGTCTGCT11.0No Hit
ATGGTCCCAACTGGTTTGTCTGATATGAGT11.0No Hit
TAGGAAATTCGTCGGCCACACAAGTGTG11.0No Hit
AGGAAGCGTAAACCCAGAGAACCGTTGGC11.0No Hit
ATCCCTAGGAAATTCGTCGGCCTCACAAGT11.0No Hit
TCCACCCAAGATCAAAATTTTGTAAACTGTT11.0No Hit
TACTGAAGGAACTGGGGAAACTGTGTCT11.0No Hit
TAGGAAATTCGTCGGCCACACAAGTGT11.0No Hit
TACAACTGTGGAACGGGGCGATTGGGAC11.0No Hit
AATACAACTGTGGAACGGGGCGATTGGGA11.0No Hit
TTCCACCGAAGATCAAAATTTTGTAAACTGT11.0No Hit
TGCGGTACAGGGCAATCATTCTATCGGCC11.0No Hit
TCTACGTAGCGGAGGAGGTGCGAGTGCT11.0No Hit
TCAGGGGTCGGCAACTTTTTCAGAGC11.0No Hit
TAGGAAGCGTAAACCCAGAGAACCGTTGGC11.0No Hit
TTACTCCTTTGTCGTATATACCTGTAGACT11.0No Hit
TACATAAGAAGTGCAATGGTTCCACCTAGG11.0No Hit
TCAAACTCCACCTTACCTTTTCGTCAATGG11.0No Hit
CACAATGTCGGCGACACAGGTGGCGAGTCTT11.0No Hit
TCCTACGGAATCAAGCTCAACAGACCCTTC11.0No Hit
GCGAGTGCTGTATCCCTAGGAAATTCGT11.0No Hit
TACAAATCCTACGGAATCAAGCTCAACAGA11.0No Hit
TAAATTTTGCGAGGTAGACGTAACGTCGGC11.0No Hit
TAGGAAATTCGTCGGCCTCACAAGTGTGGATA11.0No Hit
CCACAAGTTCATGTTGGTATCAGCGCTAT11.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)