FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.2812.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2812.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length23-33
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTGAAGAAACTGTGCCCGGA412.903225806451612No Hit
CCTGTGAAGAAACTGTGCCCGGAGAA39.67741935483871No Hit
TCCCAGACCGACGGCCCCTGTGAAGAAACT39.67741935483871No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGC26.451612903225806No Hit
CTGTGAAGAAACTGTGCCCGGAGAAA26.451612903225806No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAA26.451612903225806No Hit
CCTGTGAAGAAACTGTGCCCGGAGA26.451612903225806No Hit
TTAGGCAGGCAAGTAGAATGGTATAAAGC13.225806451612903No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAAAGC13.225806451612903No Hit
TGCCTGAACCTGCATCCCAAAAACCAAGAC13.225806451612903No Hit
CTGTGAAGAAACTGTGCCCGGAGA13.225806451612903No Hit
GCCCCTGTGAAGAAACTGTGCCCGGAG13.225806451612903No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAA13.225806451612903No Hit
TTTTAGAACTTCCTTCATCCATTGAAAGC13.225806451612903No Hit
TAGGCAGGCAAGTAGAATGGTATAAAGCT13.225806451612903No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGA13.225806451612903No Hit
GCCCCTGTGAAGAAACTGTGCCCGGAGAAAGCT13.225806451612903No Hit
TAGGCAGGCAAGTAGAATGGTATAAAGCA13.225806451612903No Hit
CCCCTGTGAAGAAACTGTGCCCGGA13.225806451612903No Hit
TTCGGAACCACCCTGCTGCGCCGTTGAA13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)