FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.2812.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2812.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849
Sequences flagged as poor quality0
Sequence length19-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1156.219578150351541No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGT1075.78691184424013No Hit
TACTTAATTGTGAACTCATGCCGGGGAA713.839913466738778No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC603.244997295835587No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC542.9204975662520285No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA502.704164413196322No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG492.650081124932396No Hit
TAACTCTTCGTCCGACTCGACATCCATA372.0010816657652786No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT311.6765819361817198No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC281.5143320713899404No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT271.4602487831260142No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG241.2979989183342346No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA241.2979989183342346No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT241.2979989183342346No Hit
TCACAGGGGACGTCGGTCTGGGACACTC221.1898323418063819No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC221.1898323418063819No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC211.1357490535424553No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC180.9734991887506761No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA170.9194159004867496No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT170.9194159004867496No Hit
TACTTAATTGTGAACTCATGCCGGGGA170.9194159004867496No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT160.8653326122228232No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT150.8112493239588967No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC140.7571660356949702No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC140.7571660356949702No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT140.7571660356949702No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG130.7030827474310438No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT120.6489994591671173No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG120.6489994591671173No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT110.5949161709031909No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT110.5949161709031909No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC110.5949161709031909No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC110.5949161709031909No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC110.5949161709031909No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG100.5408328826392644No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC100.5408328826392644No Hit
TACTTAATTGTGAATTCATGCCGGGGAA100.5408328826392644No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC100.5408328826392644No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT90.48674959437533805No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACTT90.48674959437533805No Hit
TGGGCACATCTGCACTGGTTGGATTGATA90.48674959437533805No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC90.48674959437533805No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG80.4326663061114116No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT80.4326663061114116No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC80.4326663061114116No Hit
TACTTAATTGTGAATTCATGCCGGGGA80.4326663061114116No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.4326663061114116No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCT70.3785830178474851No Hit
TTTGAAGAATTCTAGCACCACGATGAAAT70.3785830178474851No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT70.3785830178474851No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT70.3785830178474851No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT70.3785830178474851No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC70.3785830178474851No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC60.32449972958355866No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA60.32449972958355866No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG60.32449972958355866No Hit
TGAAACCGTGCACAATGTCTTCCAAGTTGC60.32449972958355866No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT60.32449972958355866No Hit
TAGATATGGGCACATCTGCACTGGTTGGA60.32449972958355866No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC60.32449972958355866No Hit
TGCTGGTAGATATGGGCACATCTGCACTG50.2704164413196322No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT50.2704164413196322No Hit
TACAGGGTCAATTGTTGCGTAGTAATCTCC50.2704164413196322No Hit
TAGTCGGCTTCTTTCATAATTATTTGATGC40.2163331530557058No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT40.2163331530557058No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC40.2163331530557058No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.2163331530557058No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT40.2163331530557058No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAA40.2163331530557058No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAAT40.2163331530557058No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCT40.2163331530557058No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT40.2163331530557058No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGGG40.2163331530557058No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGA40.2163331530557058No Hit
TCGATGAAATAGTGGGGGCAGCAGACCT40.2163331530557058No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG40.2163331530557058No Hit
TGCACTTTGGCTCCGGATGCTTTCTC40.2163331530557058No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT40.2163331530557058No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG40.2163331530557058No Hit
TATGGGCACATCTGCACTGGTTGGATT40.2163331530557058No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG40.2163331530557058No Hit
TGAGTACAGTTTGAAGAATTCTAGCACC40.2163331530557058No Hit
TTGGTCCAGTGAGGCGTTTGAAATATGAGC40.2163331530557058No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT40.2163331530557058No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG40.2163331530557058No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT40.2163331530557058No Hit
TTCGTCCGACTCGACATCCATAATTGGCG40.2163331530557058No Hit
GAGTAATTGTTCTCCCGTATTTCTGGGGC40.2163331530557058No Hit
TATGAGCTTCAACGGCGCAGCAGGGTG40.2163331530557058No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA40.2163331530557058No Hit
TTGAAATATGAGCTTCAACGGCGCAGCAGGG30.16224986479177933No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTA30.16224986479177933No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGT30.16224986479177933No Hit
TGATATTGACCCAACTAGACTAGTAGAACT30.16224986479177933No Hit
TAGATATGGGCACATCTGCACTGGTTGG30.16224986479177933No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGT30.16224986479177933No Hit
TGCTTCCAGTAGACTTCCTTCGGTTG30.16224986479177933No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCC30.16224986479177933No Hit
TTCGGCCCATAGCATTGACATTTTCTTCATC30.16224986479177933No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTCT30.16224986479177933No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGAC30.16224986479177933No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGAC30.16224986479177933No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGG30.16224986479177933No Hit
TCGGTCTGGGACACTCTTGGAAGGTCA30.16224986479177933No Hit
TGCACTGGTTGGATTGATAGGAACAGTC30.16224986479177933No Hit
TCCAGATTCGCATGCTGACGCGACCACGCT30.16224986479177933No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA30.16224986479177933No Hit
ACAGTTTGAAGAATTCTAGCACCACAATG30.16224986479177933No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC30.16224986479177933No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC30.16224986479177933No Hit
TTCATCTCCAGATTCGCATGCTGACGCGACC30.16224986479177933No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG30.16224986479177933No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGG30.16224986479177933No Hit
CCCCGTCTGCGCACTGGATGAAACCGTGC30.16224986479177933No Hit
TACAGGGTCAATTGTTGCATAGTAATCTCC30.16224986479177933No Hit
TGTATAACGTGGACGTGGTCGTGGTGTT30.16224986479177933No Hit
TTAGATTGATAATTCCCTGAGGATGTTCCTCCC30.16224986479177933No Hit
TATGGGCACATCTGCACTGGTTGGA30.16224986479177933No Hit
TGTAAAACGTTTGCTTTGACAGGTATCCT30.16224986479177933No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTC30.16224986479177933No Hit
TACTTAATTGTGAACTCATGCCGGGGAATA30.16224986479177933No Hit
TTCGCATGCTGACGCGACCACGCTGCTGC30.16224986479177933No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG30.16224986479177933No Hit
TTCCAGTAGACTTCCTTCGGTTGACCTACA30.16224986479177933No Hit
ACTTGCACTTTGGCTCCGGATGCTTTCTC20.1081665765278529No Hit
TACTTAATTGTGAATTCATGCCGGGGAAT20.1081665765278529No Hit
TGGTAGATATGGGCACATCTGCACTGGTTG20.1081665765278529No Hit
CTCGACATCCATAATTGGCGGTTTGCTG20.1081665765278529No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAT20.1081665765278529No Hit
GCTTGCTTCCAGTAGACTTCCTTCGGTT20.1081665765278529No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAA20.1081665765278529No Hit
TAGTAATTGTTCTCCCGTATTTCTGGGGC20.1081665765278529No Hit
ACTTAATTGTGAACTCATGCCGGGGAA20.1081665765278529No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGG20.1081665765278529No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGCCT20.1081665765278529No Hit
TATGGGCACATCTGCACTGGTTGGATTG20.1081665765278529No Hit
TCTCAACAGTTGCCCCGTCTGCGCACT20.1081665765278529No Hit
TCACAGGGGCCGTCGGTCTGGGACACT20.1081665765278529No Hit
TAACTCTTCGTCCGACTCGACATCCAT20.1081665765278529No Hit
TGCTGGTAGATATGGGCACATCTGCACT20.1081665765278529No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAA20.1081665765278529No Hit
TTAGATTGATAATTCCCTGAGGATGTGA20.1081665765278529No Hit
TCACAGGGGACGTCGGTCTGGGACACTCT20.1081665765278529No Hit
TAGAGATGGGCACATCTGCACTGGTTGGAT20.1081665765278529No Hit
TCTCAACAGTTGCCCCGTCTGCGCACTG20.1081665765278529No Hit
TTCCAAATAGAGCACGGAATTGACGGCATA20.1081665765278529No Hit
TAGATATGGGCACATCTGCACTGGTTG20.1081665765278529No Hit
TCCAGTAGACTTCCTTCGGTTGACCTACA20.1081665765278529No Hit
TCGTGTATAACGTGGACGTGGTCGTGGTG20.1081665765278529No Hit
TCTCCAGATTCGCATGCTGACGCGACCACGCTGC20.1081665765278529No Hit
TCGATTTGGTCCAGTGAGGCGTTTGAAAT20.1081665765278529No Hit
TGCACTTGCACTTTGGCTCCGGATGCTTTC20.1081665765278529No Hit
TGACCCAACTAGACTAGTAGAACTATTTA20.1081665765278529No Hit
TTGGTCCTTTTTCCAAATAGAGCACGGAAT20.1081665765278529No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCGT20.1081665765278529No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATCA20.1081665765278529No Hit
TAGATTGATAATTCCCTGAGGATGTGAAG20.1081665765278529No Hit
TTCATGCCGGGGAATAACTCTTCGTCCGAC20.1081665765278529No Hit
GACATCCATAATTGGCGGTTTGCTGGTA20.1081665765278529No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATT20.1081665765278529No Hit
TTCATGCCGGGGAATAACTCTTCGTCC20.1081665765278529No Hit
TCGTGTATAACGTGGACGTGGTCGTGG20.1081665765278529No Hit
ACGCTGCTGCTCGATGAAATAGTGGGG20.1081665765278529No Hit
ACTTAATTGTGAATTCATGCCGGGGA20.1081665765278529No Hit
TTAGATTGATAATTCCCTGAGGATGTGAAG20.1081665765278529No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTGG20.1081665765278529No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCT20.1081665765278529No Hit
TGCACTTTGGCTCCGGATGCTTTCTCC20.1081665765278529No Hit
TTCCGAACGAACTCGAGTAATTGTTCTCCC20.1081665765278529No Hit
TGCTGATCGGGAGTCAAATGCACTTGCAC20.1081665765278529No Hit
TGTGAACTCATGCCGGGGAATAACTCTTCGT20.1081665765278529No Hit
GATGAAATAGTGGGGGCAGCAGACCTATA20.1081665765278529No Hit
TAGATCGTCTGGAACTTGGTAAGAGATA20.1081665765278529No Hit
TTGAAATATGAGTTTCAACGGCGCAGCA20.1081665765278529No Hit
ATACTTAATTGTGAACTCATGCCGGGGAA20.1081665765278529No Hit
TCTCCAGATTCGCATGCTGACGCGACCACG20.1081665765278529No Hit
TGTTCTGCGATGACGGTAAATGCGTTATT20.1081665765278529No Hit
TCCATAATTGGCGGTTTGCTGGTAGA20.1081665765278529No Hit
TTGGCGGTTTGCTGGTAGATATGGGCAC20.1081665765278529No Hit
TCAACAGTTGCCCCGTCTGCGCACTGGATG20.1081665765278529No Hit
TTGTTGAGTACAGTTTGAAGAATTCTAGC20.1081665765278529No Hit
TTGGTATAGTCGGCTTCTTTCATAATTAT20.1081665765278529No Hit
GTTTGAAGAATTCTAGCACCACGATGAA20.1081665765278529No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTCC20.1081665765278529No Hit
TCCATTAGTGACCAAGCCGCCAATTCAAT20.1081665765278529No Hit
TCATCTCCAGATTCGCATGCTGACGCGACA20.1081665765278529No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACAC20.1081665765278529No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCT20.1081665765278529No Hit
TAATTGTGAACTCATGCCGGGGAATAAC20.1081665765278529No Hit
TTAATTGTGAACTCATGCCGGGGAATAACT20.1081665765278529No Hit
TTCTTCACAGGGGACGTCGGTCTGGGACACT20.1081665765278529No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTCAAC20.1081665765278529No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCATA20.1081665765278529No Hit
TACAGGGTCAATTGTTGCATAGTAATCTC20.1081665765278529No Hit
TTCACAGGGGCCGTCGGTCTGGGACACT20.1081665765278529No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCC20.1081665765278529No Hit
TTGAAATATGAGCTTCAACGGCGCAGCA20.1081665765278529No Hit
TAGCAACCAAAGAAACATCTACAGACATGT20.1081665765278529No Hit
TAATTGTGAATTCATGCCGGGGAATAACT20.1081665765278529No Hit
TCTCCGGGCACAGTTTCTTCACAGGGGCC20.1081665765278529No Hit
TCCTTCGGTTGACCTACACATTTCGAGT20.1081665765278529No Hit
TTCGGCCCATAGCATTGACATTTTCTTCATCT20.1081665765278529No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACTC20.1081665765278529No Hit
TGCCCCGTCTGCGCACTGGATGAAACCCTGA20.1081665765278529No Hit
TGATAGGAACAGTCGTGTATAACGTGGAC20.1081665765278529No Hit
TTATAGCAACCAAAGAAACATCTACAGAC20.1081665765278529No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTTGACA20.1081665765278529No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGAG20.1081665765278529No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTC20.1081665765278529No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTT20.1081665765278529No Hit
GTACTCCTCGTGTATATATTGTTGTAGATC20.1081665765278529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)