FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.2829.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2829.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409
Sequences flagged as poor quality0
Sequence length18-35
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGACGGAGCACCTGACCCTGCCAGG4210.268948655256724No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCA266.356968215158925No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCA194.645476772616137No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAA153.6674816625916873No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGC153.6674816625916873No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGCCC122.93398533007335No Hit
ATAGGACGGAGCACCTGACCCTGCCAG122.93398533007335No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGC112.689486552567237No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAGCC102.444987775061125No Hit
ATAGGACGGAGCACCTGACCCTGCCA92.2004889975550124No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGC81.9559902200488997No Hit
TCTACCCACTCTCGTTGCTCCTCTGGCATA71.7114914425427872No Hit
TAGGACGGAGCACCTGACCCTGCCAGG71.7114914425427872No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGT71.7114914425427872No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAAG71.7114914425427872No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCC71.7114914425427872No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAA61.466992665036675No Hit
TACCCACTCTCGTTGCTCCTCTGGCATAG51.2224938875305624No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAC51.2224938875305624No Hit
TAACAATGGTCAATCGCTAAAAGAACGCT51.2224938875305624No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCCTCTG51.2224938875305624No Hit
CACTCTCGTTGCTCCTCTGGCATAGCCCCAC51.2224938875305624No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGCATA40.9779951100244498No Hit
TGTGTTGTTTTACCCACTCTCGTTGCTCCTC40.9779951100244498No Hit
TCTCGTTGCTCCTCTGGCATAGCCCCACAG40.9779951100244498No Hit
TGTTCTACCCACTCTCGTTGCTCCTCTGGC30.7334963325183375No Hit
ATAGGACGGAGCACCTGACCCTGCC30.7334963325183375No Hit
TGCAACGATAGGTAGGAAATCCTCATGGAT30.7334963325183375No Hit
TCTACCCACTCTCGTTGCTCCTCTGGCATAG30.7334963325183375No Hit
TGTAGTAATAAGCCAGGCTAAGGAGGT30.7334963325183375No Hit
ATAACAATGGTCAATCGCTAAAAGAACTCCGT30.7334963325183375No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGTG30.7334963325183375No Hit
TTGTTCTACCCACTCTCGTTGCTCCTCTG30.7334963325183375No Hit
AATAGGACGGAGCACCTGACCCTGCCAGGCACA30.7334963325183375No Hit
TTCTACCCACTCTCGTTGCTCCT30.7334963325183375No Hit
TGTGTTGTTCTACCCACTCTTGTTGCTCC20.4889975550122249No Hit
TCCATGCTATCCTGGGTTCCCCACACTGG20.4889975550122249No Hit
TAGGACGGAGCACCCGACCCTGCCAGGCA20.4889975550122249No Hit
TACTCCGTTAGTAGAGCTCCATGCTATCCT20.4889975550122249No Hit
AAAGAAACAGTTAAGGGAGGACCACTC20.4889975550122249No Hit
ACTCTCGTTGCTCCTCTGGCATAGCCCCACA20.4889975550122249No Hit
AACAATGGTCAATCGCTAAAAGAACTCCGT20.4889975550122249No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAC20.4889975550122249No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCCT20.4889975550122249No Hit
CCAATTTAATTACAATAGTCAGTCAGT20.4889975550122249No Hit
TTCTACCCACTCTCGTTGCTCCTCTGGCATAGCCC20.4889975550122249No Hit
TCTCGTTGCTCCTCTGGCATAGCCC20.4889975550122249No Hit
TGTGGTCAATCCCATGCACCCTCCCCAAAG20.4889975550122249No Hit
ACTCTGTGTTGTTCTACCCACTCTC20.4889975550122249No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCATA20.4889975550122249No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAAGT20.4889975550122249No Hit
TGTTCTACCTACTCTCGTTGCTCCT20.4889975550122249No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGG20.4889975550122249No Hit
CCCACTCTCGTTGCTCCTCTGGCATAGCC20.4889975550122249No Hit
TGTGTTGTTTTACCCACTCTCGTTGCTCCT20.4889975550122249No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAAG20.4889975550122249No Hit
TAAAAGAACGCTGTGTTGTTCTACCCACTCTCGT20.4889975550122249No Hit
GTTGTTCTACCCACTCTCGTTGCTCCT20.4889975550122249No Hit
ATCCTGGGTTCCCAACACTGGCAATCACTTATAAC20.4889975550122249No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGA20.4889975550122249No Hit
TCCTGGGAAAGAAACAGTTAAGGGAGGAC20.4889975550122249No Hit
TAGGACGGAGCACCTGACCCTGCCAGGC20.4889975550122249No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAAGTAC20.4889975550122249No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCACCA10.24449877750611246No Hit
TCCTGGGAAAGAAACAGTTAAGGGAGGACC10.24449877750611246No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAG10.24449877750611246No Hit
TAACAATGGTCAATCGCTAAAAGAACTCC10.24449877750611246No Hit
CCCACTCTCGTTGCTCCTCTGGCATAG10.24449877750611246No Hit
TGCAACGATAGGTAGGAAATCCTCATGGATAAA10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCATT10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGT10.24449877750611246No Hit
ATCGCTAAAAGAAAGCTGTGTTGTTCTACCCA10.24449877750611246No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAT10.24449877750611246No Hit
TGTTGTTCTACCCACTCTCGTTGCTCCTC10.24449877750611246No Hit
ATTGCAACGATAGGTAGGAAATCCTCATGGAT10.24449877750611246No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTCCTC10.24449877750611246No Hit
TTTTACCCACTCTCGTTGCTCCT10.24449877750611246No Hit
TGTGTTGTTCTACCCACTCTCGTTGCTC10.24449877750611246No Hit
CGCTGTGTTGTTCTACCCACTCTCGTTGCTCCTCT10.24449877750611246No Hit
TGGGAAAGAAACAGTTAAGGGAGGACCACTC10.24449877750611246No Hit
CGTACTCCGTTAGTAGAGCTCCA10.24449877750611246No Hit
CGCTGTGTTGTTCTACCCACTCTCGTTGCTCCT10.24449877750611246No Hit
TCTACCCACTCTCGTTGCTCCTCTGGCATAGC10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGGA10.24449877750611246No Hit
ATTCTAACGCGTACTCCGTTAGTAGAGCTCCA10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGCAAGTG10.24449877750611246No Hit
GTTCTACCCACTCTCGTTGCTCCTCTGGC10.24449877750611246No Hit
CTCGTTGCTCCTCTGGCATAGCCCCACA10.24449877750611246No Hit
CGTGTTGTTCTACCCACTCTCGTTGCTCCT10.24449877750611246No Hit
TCCATGCTATCCTGGGTTCCCCACACTG10.24449877750611246No Hit
ATAGGACGGAGCACCTGA10.24449877750611246No Hit
TGCAATAGGACGGAGCACCTGACCCTGCCAGGCA10.24449877750611246No Hit
CTACCCACTCTCGTTGCTCCTCTGGCATAGCCC10.24449877750611246No Hit
TAGGAAATCCTCATGGATAAATGGAAGACCCCGGA10.24449877750611246No Hit
CCCACTCTCGTTGCTCCTCTGGCATAGCCCCACA10.24449877750611246No Hit
TCTCGTTGCTCCTCTGGCATAGCCCCACA10.24449877750611246No Hit
TTAGGACGGAGCACCAGACCCTGCCAGGCAAGTAC10.24449877750611246No Hit
TGTTCTACCCACTCTCGTTGCTCCTCTGGCA10.24449877750611246No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCACT10.24449877750611246No Hit
AATAGGACGGAGCACCTGACCCTGCCAGG10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGTGCA10.24449877750611246No Hit
TTGCTCCTCTGGCATAGCCCCACAGG10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGAGG10.24449877750611246No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCACC10.24449877750611246No Hit
ATAACAATGGTCAATCGCTAAAAGAACTCCG10.24449877750611246No Hit
TCAATCGCTAAAAGAACGCTGTGTTGTTTTAC10.24449877750611246No Hit
TCAATCGCTAAAAGAACGCTGTGTTGTTC10.24449877750611246No Hit
TAACGCGTACTCCGTTAGTAGAGCTCCAT10.24449877750611246No Hit
AGATCTCCGTGGGCTCCTCTCAGCCAA10.24449877750611246No Hit
TCCGTTAGTAGAGCTCCATGCTATCC10.24449877750611246No Hit
TAGGACGTAGCACCTGACCCTGCCAGGCAAG10.24449877750611246No Hit
AATAGGACGGAGCACCTGACCCTGCCAGGCAC10.24449877750611246No Hit
CTGACCCTGCCAGGCAAGTACCTACCTTTTG10.24449877750611246No Hit
TTCTAACGCGTACTCCGTTAGTAGAGCTCC10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGC10.24449877750611246No Hit
TTACCCACTCTCGTTGCTCCTCTGGCATAGC10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTG10.24449877750611246No Hit
TTCCCCACACTGGCAATCACTTATACAATG10.24449877750611246No Hit
CTAAAAAAACGCTGTGTTGTTCTACCCACT10.24449877750611246No Hit
TGTGTTGTTCTACCTACTCTCGTTGCTCC10.24449877750611246No Hit
ATAGGACGGAGCACCTGACCCTGCCAGGTGC10.24449877750611246No Hit
TTCTACCCACTCTCGTTGCTCCTCTG10.24449877750611246No Hit
ACGCGTACTCCGTTAGTAGAGCTCCATGCTATCCT10.24449877750611246No Hit
TGTGTTGTTTTACCCACTCTCGTTGCTCC10.24449877750611246No Hit
CACTTATAACAATGGTCAATCGCTAAAAG10.24449877750611246No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCACTA10.24449877750611246No Hit
TAGGACGGAGCACCTGACCCTGCCAGGCAAGT10.24449877750611246No Hit
TTGTTCTACCCACTCTCGTTGCTCCTCTGGC10.24449877750611246No Hit
AGAAGTTAGACCCTACTGTAGTAATAAG10.24449877750611246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)