FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.2854.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2854.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108
Sequences flagged as poor quality0
Sequence length21-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATACGAAACTGCATCGTGGCACTCC109.25925925925926No Hit
AGATACGAAACTGCATCGTGGCACTCCTTCAAGGA54.62962962962963No Hit
CACTTCAACTACCTCGCCAACTGTTATCGC54.62962962962963No Hit
AGATACGAAACTGCATCGTGGCACTCCT43.7037037037037033No Hit
AGATACGAAACTGCATCGTGGCACTC43.7037037037037033No Hit
TATTCGGCTGCCGCCACGAAGAACAAGAAGGACTG43.7037037037037033No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGC43.7037037037037033No Hit
CCGGTTCAGACGATGAACAATTTCCTGGC43.7037037037037033No Hit
TGGACCATGAGAATCTAATATTTGCCGCGCC32.7777777777777777No Hit
ATCTAGAACACCAGGTCACCCCGAGC32.7777777777777777No Hit
AAAAAGCACAGGATAGAACTCAATGGAGAG32.7777777777777777No Hit
GACGCCTCGACAAACAGTGGAGCTTCTGCC32.7777777777777777No Hit
CACATGGATCCAAAAAGCACAGGATAGAACTCAA21.8518518518518516No Hit
TCCTGGCCTATCTGTTTCGTGTGGACGCC21.8518518518518516No Hit
ACAGATACGAAACTGCATCGTGGCACTC21.8518518518518516No Hit
ACATTACAGATCATCAAAGAGAAGCTTGGGGAC21.8518518518518516No Hit
CCTCGACAAACAGTGGAGCTTCTGCCAGC21.8518518518518516No Hit
GCGCCGCTAACTTCCATTCTACAAACTC21.8518518518518516No Hit
TCCACGATTGCGCCTACACAAGCTCCAC21.8518518518518516No Hit
ATGGAGAGGAAATGAAGAGGCCTTGGTC21.8518518518518516No Hit
AAGCACAGGATAGAACTCAATGGAAGT21.8518518518518516No Hit
TCCTGGCCTATCTGTTTCGTGTGGACG21.8518518518518516No Hit
CTTCAAGTCGGAGGGCGTACATGGCGATT10.9259259259259258No Hit
TCGACAAACAGTGGAGCTTCTGCCAGC10.9259259259259258No Hit
ACAAGAAGGACTGGTGGCAAGA10.9259259259259258No Hit
AAACTTCCAAGATTGCCTCAGCAAACAGT10.9259259259259258No Hit
CCAAAAAGCACAGGATAGAACTCAATGGAGAG10.9259259259259258No Hit
TCAGGTCACCAGAAGGAACGAAACATTACAGAT10.9259259259259258No Hit
AGGACGTGGAGTTGAAACGTCAGGTCACCA10.9259259259259258No Hit
CCTTGCTCTATCTCCAAAAAGGTCGGC10.9259259259259258No Hit
TCTATACCGACAGCTGCAAAATTCGCC10.9259259259259258No Hit
GGGACAGATACGAAACTGCATCGTGGCACTC10.9259259259259258No Hit
TATTCGGCTGCCGCCACGAAGAACAAGAAGGAC10.9259259259259258No Hit
TCGGCTGCCGCCACGAAGAACAAGAAGGACTG10.9259259259259258No Hit
AAGTTAGATAAGGCGGGTCAGGGCATT10.9259259259259258No Hit
CAAAAAGCACAGGATAGAACTCAATGGAG10.9259259259259258No Hit
TCACATGGATCCAAAAGGCACAGGATAGAACTCAA10.9259259259259258No Hit
CCAAAGACGATGAACAATTTCCTGGCCT10.9259259259259258No Hit
CAAACACAGAAGACGAGTGGGGAGCCT10.9259259259259258No Hit
TCCTGGCCTATCTGTTTCGTGTGGACA10.9259259259259258No Hit
TTTGCGGCGAATTCGGCATTGGGTCGTG10.9259259259259258No Hit
TCAGTTTGTTTCCTTTCACGGCGACCACCTCCA10.9259259259259258No Hit
ATAGAACTCAATGGAGAGGAAATGAAGAGGCCT10.9259259259259258No Hit
GGACCACCCAACAGCGCCGCTAACTTC10.9259259259259258No Hit
TGGGGAGCCTGGAACACTTGTTAACAACGG10.9259259259259258No Hit
ATTCGGCTGCCGCCACGAAGAACAAGAAGG10.9259259259259258No Hit
CGGACTTTTCGCTTACACCCAGC10.9259259259259258No Hit
TACTTTGGGGACAGACGAGATAATAGGGT10.9259259259259258No Hit
TTTGCGGCGAATTCGGCATTGGGTCGTGC10.9259259259259258No Hit
TCAGACGATGAACAATTTCCTGGCCTATC10.9259259259259258No Hit
GGAGGGCGTACATGGCGATTG10.9259259259259258No Hit
TTCAGACGATGAACAATTTCCTGGCCG10.9259259259259258No Hit
AGTCACATGGATCCAAAAAGCACAG10.9259259259259258No Hit
ACAAATACGAGGCGCTTGTAGAAGC10.9259259259259258No Hit
AGATCTAGAACACCAGGTCACCCCGAGC10.9259259259259258No Hit
TCACATGGGTCCAAAAAGCACAGGATAGAACTCA10.9259259259259258No Hit
ACAAGAAGGACTGGTGGCAAGAAGCCA10.9259259259259258No Hit
ATTCGGCTGCCGCCACGAAGAACAAGAAGGACT10.9259259259259258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)