FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.2854.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2854.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089
Sequences flagged as poor quality0
Sequence length17-35
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACGGCGCTGTTGGGTGGTCCCGAAGCC11110.192837465564738No Hit
TGAACCGGAACCACAGAACTCAGAGTCACT555.05050505050505No Hit
TATACGTCGTGTATGATGCGAGGCCTTGG373.3976124885215793No Hit
TAACGGCGCTGTTGGGTGGTCCCGAAGCC333.0303030303030303No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTACC232.1120293847566574No Hit
TCTGAACCGGAACCACAGAACTCAGAGTC191.7447199265381086No Hit
TACCCAATCGCCATGTACGCCCTCCGACT181.6528925619834711No Hit
TTAACGGCGCTGTTGGGTGGTCCCGAAG181.6528925619834711No Hit
TACGGCGGTAACAATGTCCCAGAGGCTGC151.3774104683195594No Hit
TGAACCGGAACCACAGAACTCAGAGTCACTG131.1937557392102847No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTG121.1019283746556474No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACCGG121.1019283746556474No Hit
TCTAGATCTGCCGAGAACTTTCCCTTACC111.0101010101010102No Hit
TCGTCTGAACCGGAACCACAGAACTCAG100.9182736455463728No Hit
TACCCTATTATCTCGTCTGTCCCCAAAGT90.8264462809917356No Hit
ACCGGAACCACAGAACTCAGAGTCACTGTT90.8264462809917356No Hit
TTTGTAGAATGGAAGTTAGCGGCGCTGTT90.8264462809917356No Hit
TTAACGGCGCTGTTGGGTGGTCCCGAAGC80.7346189164370982No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGG80.7346189164370982No Hit
TGTTTGAGGACTCCACGCTGAAATGCCCT80.7346189164370982No Hit
TCTTATAATACGCCAGCGAAGGATCGTTCC80.7346189164370982No Hit
TCAAAAAAGAAGTTCTTTCCCGCGTTGGA70.642791551882461No Hit
TACCTGTCCACTGCTGTACGAAGGCCTCT70.642791551882461No Hit
TTTGACTACCCAATCGCCATGTACGCCCTC70.642791551882461No Hit
TTGTAGAATGGAAGTTAGCGGCGCTGTT60.5509641873278237No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAACC60.5509641873278237No Hit
TACGCCCTCCGACTTGAAGGTACACAGCCT60.5509641873278237No Hit
TTGTCGCATTAAGAAAAGATTCCCTACCC60.5509641873278237No Hit
TCCCTTACCATACTTCCACGACAAACAGCT60.5509641873278237No Hit
TATACGTCGTGTATGATGCGAGGCCTTGGA60.5509641873278237No Hit
TCCACGCTGAAATGCCCTGACCCGCCTTATC60.5509641873278237No Hit
TGTTCCAGGCTCCCCACTCGTCTTCTGTGT60.5509641873278237No Hit
TAACGGCGCTGTTGGGTGGTCCCGAAG60.5509641873278237No Hit
TCAGCCGTTACCTGTCCACTGCTGGACGAA60.5509641873278237No Hit
TTCTAGATCTGCCGAGAACTTTCCCTTAC60.5509641873278237No Hit
TCGTCTGAACCGGAACCACAGAACTCAGA60.5509641873278237No Hit
TTATACAACCTCCCCACCAATAGCCAACAT50.4591368227731864No Hit
TTTTTCAACAAGGTTCTTGGTTGGTTGGG50.4591368227731864No Hit
TCCCAGAGGCTGCTCCGACAAACATTACAT50.4591368227731864No Hit
TGAGGACTCCACGCTGAAATGCCCTGACCC50.4591368227731864No Hit
TCAGAGTCACTGTTTGCTGAGGCAATCTT50.4591368227731864No Hit
TGAGGACTCCACGCTGAAATGCCCTGACC50.4591368227731864No Hit
GAGGACTCCACGCTGAAATGCCCTGACCCG40.3673094582185491No Hit
TAGGCACAATCGTGGACGACGTGGAGGT40.3673094582185491No Hit
AACCACAGAACTCAGAGTCACTGTTTGCTG40.3673094582185491No Hit
TGTTAACAAGTGTTCCAGGCTCCCCACTC40.3673094582185491No Hit
TAAAAGGATCTGTTCGGGTAATGAGTGTC40.3673094582185491No Hit
TTACCTAAATTTCCTCTACCATTCCTCGG40.3673094582185491No Hit
TACGCCAGCGAAGGATCGTTCCCTAAACTC40.3673094582185491No Hit
TTTTCAACAAGGTTCTTGGTTGGTTGGGA40.3673094582185491No Hit
TGATTGTCGTCGTCGTGGTCAGCCGTTA40.3673094582185491No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTC40.3673094582185491No Hit
TTTGCGCCGAACCCGTTACATAGTTAGATCTC40.3673094582185491No Hit
TCCACGCTGAAATGCCCTGACCCGCCT40.3673094582185491No Hit
GAACCGGAACCACAGAACTCAGAGTCACT40.3673094582185491No Hit
TTCCCTACCCGGATGTATCTGTTTATCGACT40.3673094582185491No Hit
TCGGTTATTACTCTAAATGCCCCGCAAAAC40.3673094582185491No Hit
TGAACCGGAACCACAGAACTCAGAGTCACTGT40.3673094582185491No Hit
TGCGCCGAACCCGTTACATAGTTAGATCTC40.3673094582185491No Hit
TGCAGAAATACCGCTGGCAGAAGCTCCACT40.3673094582185491No Hit
TAGGCCAGGAAATTGTTCATCGTCTGAAC40.3673094582185491No Hit
TCCGAATCGATATTGTCGAATAACCCACCT40.3673094582185491No Hit
TAGAATACAGCACCAGCACCAGCAGATGA30.27548209366391185No Hit
TGAACCGGAACCACAGAACTCAGAGTCAC30.27548209366391185No Hit
TACGTCGTGTATGATGCGAGGCCTTGG30.27548209366391185No Hit
TACGTCGTGTATGATGCGAGGCCTTGGA30.27548209366391185No Hit
TACCCAATCGCCATGTACGCCCTCCGACTT30.27548209366391185No Hit
TTCCCTACCCGGATGTATCTGTTTATCGAC30.27548209366391185No Hit
TACCTACCAAGCCGAAGTTACAATAAAAAT30.27548209366391185No Hit
TTCGCCGCAAAAGTCACCGTCGTTCTCATAGT30.27548209366391185No Hit
TACATATACCCTATTATCTCGTCTGTCCCC30.27548209366391185No Hit
TTCCACGAGTGCCACATAATCCCTAAGGG30.27548209366391185No Hit
TCTGAACCGGAACCACAGAACTCAGAGTCAC30.27548209366391185No Hit
TTATCTCGTCTGTCCCCAAAGTACATAATT30.27548209366391185No Hit
TAAGTTCGGTACGACTTTCGGAAGAGGT30.27548209366391185No Hit
TACACGTGCCTCGCGATAACAGTTGGCG30.27548209366391185No Hit
CTGAACCGGAACCACAGAACTCAGAGTCACT30.27548209366391185No Hit
TATAGAATACAGCACCAGCAGCAGCAGAT30.27548209366391185No Hit
TCTGAACCGGAACCACAGAACTCAGAGTCACT30.27548209366391185No Hit
TTAGAGAGAGGACCCAACAACATTGTCGC30.27548209366391185No Hit
TAATGAGTGTCTTTTCTGAGTGGTCCCACC30.27548209366391185No Hit
TACGTCGTGTATGATGCGAGGCCTTGGAG30.27548209366391185No Hit
TCATCTTCTCCACCTAGTCCCTCAAATCT30.27548209366391185No Hit
TTCCCTTACCATACTTCCACGACAAACAGC30.27548209366391185No Hit
CAGGCTCCCCACTCGTCTTCTGTGT30.27548209366391185No Hit
TATGGACACCCGGCTGGGCCTCTCTCA30.27548209366391185No Hit
ACCGGAACCACAGAACTCAGAGTCACTGT30.27548209366391185No Hit
TCCTTCTTGTTCTTCGTGGCGGCAGCCGA30.27548209366391185No Hit
TTGAGGACTCCACGCTGAAATGCCCTGACC30.27548209366391185No Hit
TATACCCTATTATCTCGTCTGTCCCCAAAG30.27548209366391185No Hit
ATATCGTCGTCGTGGTCAGCCATTACCTGTCCA30.27548209366391185No Hit
TATAGAATACAGCACCAGCAGCAGCAGATG20.18365472910927455No Hit
AACGATCCGACCTGTTGTATCGACACCCGGC20.18365472910927455No Hit
TCCCTAATGAACGGAGCCAGCTGCATCTC20.18365472910927455No Hit
TTCCCTTACCATACTTCCACGACAAACAG20.18365472910927455No Hit
TGAACCGGAACCACAGAACTCAGAGTCACTGTT20.18365472910927455No Hit
TCTGCCGAGAACTTTCCCTTACCATACTTCC20.18365472910927455No Hit
TCCCACCTCCCCCGCCTGAAAGTGGTCCATC20.18365472910927455No Hit
TTGATTGTCGTCGTCGTGGTCAGCCGTT20.18365472910927455No Hit
TACCTGTTGAAATTACCTACCAAGCCGAAG20.18365472910927455No Hit
TACCCGGATGTATCTGTTTATCGACTAAGT20.18365472910927455No Hit
TACTTCCACGACAAACAGCTGACCGCTCTT20.18365472910927455No Hit
TATAGAATACAGCACCAGCACCAGCAGAT20.18365472910927455No Hit
TATCGACACCCGGCTGGGCCTCTCTCAGT20.18365472910927455No Hit
TGACCGCTCTTATCAACTTCTCCGAATACC20.18365472910927455No Hit
TCTTGCCACCAGTCCTTCTTGTTCTTCGT20.18365472910927455No Hit
TGAACCGGAATCACAGAACTCAGAGTCACT20.18365472910927455No Hit
TTTGACTACCCAATCGCCATGTACGCCC20.18365472910927455No Hit
TGAACGGAGCCAGCTGCATCTCACCCTCTT20.18365472910927455No Hit
TTATAATACGCCAGCGAAGGATCGTTCCC20.18365472910927455No Hit
TTCAACGTTTCTAAGGCCGTCGGTTCAGCT20.18365472910927455No Hit
TCTTCATTTCCTCTCCATTGAGTTCTATCC20.18365472910927455No Hit
TGTTTGAGGACTCCACGCTGAAATGCCC20.18365472910927455No Hit
TTTTCCGATCCTCTTTTTATACAACCTCCC20.18365472910927455No Hit
TACGCCCTCCGACTTGAAGGTATACAGCCT20.18365472910927455No Hit
TCATAACTCTTTCTAGCCATGTCCGCATA20.18365472910927455No Hit
TTCGCCGCAAAAGTCACCGTCGTTCTCATAG20.18365472910927455No Hit
TGTTTGATCCCCACCAAGTAACGTTTTCA20.18365472910927455No Hit
TTACCTAAATTTCCTCTACCATTCCTGGG20.18365472910927455No Hit
TTCCTTCTGGTGACCTGACGTTTCAACTCCACT20.18365472910927455No Hit
GTGGCTCCCCACTCGTC20.18365472910927455No Hit
TCCCGTTGTTAACAAGTGTTCCAGGCTCCC20.18365472910927455No Hit
TGAGTGTCTTTTCTGAGTGGTCCCACCTC20.18365472910927455No Hit
TCCTTAATGTGAAGTGAAGACCATACCCTG20.18365472910927455No Hit
TGTTGTATCGACACCCGGCTGGGCCTCTCC20.18365472910927455No Hit
TTGAAAAATATGTTTCCCGGATATTGCCC20.18365472910927455No Hit
TGTGTAGGCGCAATCGTGGACGACGTGG20.18365472910927455No Hit
TGGGTCGAGTACGGAGGGCGTCTTGTATGC20.18365472910927455No Hit
TAAGAAAAGATTCCCTACCCGGATGTATC20.18365472910927455No Hit
TCCCTACCCGGATGTATCTGTTTATCGACT20.18365472910927455No Hit
TTTACCTAAATTTCCTCTACCATTCCTCGG20.18365472910927455No Hit
TGAAGACCATACCCTGTACCAAAAGAATA20.18365472910927455No Hit
AACATGGTCTCTATTCTCAGCTGCAAGGG20.18365472910927455No Hit
TCAAACACTACTCCGTTGAACCATCCGACC20.18365472910927455No Hit
TCCATTACCCTATCCGGATGCTTGCAGCCT20.18365472910927455No Hit
TCTTTTCTGAGTGGTCCCACCTCCCCCGCC20.18365472910927455No Hit
TATCCGGATGCTTGCAGCCTCTTTTAACA20.18365472910927455No Hit
TAGAATGGGCGGATTCTGTCATTGACA20.18365472910927455No Hit
TTCGATTCGGACCAATGACAAGTTTGTAGAA20.18365472910927455No Hit
TCAATATCGACAACGTGCACCGGTGCAAGGGA20.18365472910927455No Hit
TTCCCTAAACTCGCGTTCATTTCTGCCCT20.18365472910927455No Hit
TATAGAATACAGCACCAGCACCAGCAGATGATAA20.18365472910927455No Hit
AACGGCGCTGTTGGGTGGTCCCGAAGCC20.18365472910927455No Hit
TAATGAGTGTCTTTTCTGAGTGGTCCCA20.18365472910927455No Hit
AACGATCCGACCTGTTGTATCGACACCCGG20.18365472910927455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)