FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.28821.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.28821.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length15-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAAGTACCCCTGAACCTTCAGGCCCA2013.422818791946309No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATAGG74.697986577181208No Hit
TACGTCTGTATGACTCAGCCCCGTACATA64.026845637583892No Hit
TAATCGCTGTACATCTCTCTGAACCGTGG64.026845637583892No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCCT53.3557046979865772No Hit
TCGGAGCCATCAACATGCAAAGTTAAGCCT53.3557046979865772No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGGT42.684563758389262No Hit
TGATCTTCCAACTTGACCTCCTCTGGTACT42.684563758389262No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCAAT32.013422818791946No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCC32.013422818791946No Hit
TCCAATAGAACTCGTGAATCGCAGTGGGC32.013422818791946No Hit
TAGAACTCGTGAATCGCAGTGGGCTGC32.013422818791946No Hit
TGCAATGGCCGCTTGGCATAGATGGGG21.342281879194631No Hit
TGAAGAACTCTCGTGCTTGCGCTGGG21.342281879194631No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGT21.342281879194631No Hit
TCTCCCATTTCTTTACCAAGTACCCCTGAAC21.342281879194631No Hit
TCGCTGTACATCTCTCTGAACCGTGGGA21.342281879194631No Hit
TCAACGATCTTATACAGAGGCCACGCGGC21.342281879194631No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGG21.342281879194631No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGGC21.342281879194631No Hit
TACTAGTCTCTTCGCAGGAGCTGGACCGCCT21.342281879194631No Hit
TCCATGTCGCAAACCCTCTTTCTCCTCCA21.342281879194631No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCA21.342281879194631No Hit
TCCGCTGCGCTAGGTACTAGTCTCTTCGC21.342281879194631No Hit
TAACCTACCATTCTGAGACAGTTGATCTTCC21.342281879194631No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCG21.342281879194631No Hit
TCTTCCAACTTGACCTCCTCTGGTACTTG21.342281879194631No Hit
TAAGACTTTCACGATATCAACTTGTCCCT21.342281879194631No Hit
TTTCAAGGGAGATGGGCTATTCACTAGG10.6711409395973155No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGTT10.6711409395973155No Hit
TCCAACCTCCTGAGAACATCTACGCATCTT10.6711409395973155No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCCA10.6711409395973155No Hit
CTAGCAGCTTCCCGGCCTCATTTATTCCTAGGACC10.6711409395973155No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATA10.6711409395973155No Hit
TTGAAGAACTCTCGTGCTTGCGCTGG10.6711409395973155No Hit
TAGTGAAATCCGATACCCAGGTGATCTTC10.6711409395973155No Hit
TCAACATGCAAAGTTAAGCCTGGCTCAAGCT10.6711409395973155No Hit
TTTACCAAGTACCCCTGAACCTTCAGGCCC10.6711409395973155No Hit
TCTGAACCGTGGGAGATGAGTAAATGGCCTTA10.6711409395973155No Hit
TTTAACTTGCCCTGCTTCTCTCACAGACA10.6711409395973155No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCAGGCC10.6711409395973155No Hit
TCACGATATCAACTTGTCCCTTTGACACCG10.6711409395973155No Hit
TAACCTACCATTCTGAGACAGTTGATCTTC10.6711409395973155No Hit
TCTACATCGATAACTACCCCGTCATCTGG10.6711409395973155No Hit
TACAACCATCCCACCACCATCTACTCCAACCT10.6711409395973155No Hit
GAAGAACTCTCGTGCTTGCGCTGGGGT10.6711409395973155No Hit
TGCGATATGGTTTTTCAAGGGAGATGGGC10.6711409395973155No Hit
TGAACATCGACATGT10.6711409395973155No Hit
ATAGAACTCGTGAATCGCAGTGGGCTGC10.6711409395973155No Hit
ACCCATCTCCTCTAGCAGCTTCCCGG10.6711409395973155No Hit
TTCACTAAGAGTTTGTATCCAGATGTCGA10.6711409395973155No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATAGGGTA10.6711409395973155No Hit
TAGGTACTAGTCTCTTCGCAGGAGCTGGAC10.6711409395973155No Hit
TACATCGATAACTACCCCGTCATCTGG10.6711409395973155No Hit
TAGGACCTCTTTAGCTTGACTTATAACAA10.6711409395973155No Hit
TTTACCAAGTACCCCTGAACCTTCAGGCCCA10.6711409395973155No Hit
TTTGAAGAACTCTCGTGCTTGCGCTGGG10.6711409395973155No Hit
TCATACGTCTGTATGACTCAGCCCCGTA10.6711409395973155No Hit
TTGTATCCAGATGTCGAGATGAACAACTC10.6711409395973155No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCCTCAA10.6711409395973155No Hit
TTCACTAAGAGTTTGTATCCAGATGTCGAG10.6711409395973155No Hit
TATGACTCAGCCCCGTACATACAAGTCCAT10.6711409395973155No Hit
TACGTCTGTATGACTCAGCCCCGTACAT10.6711409395973155No Hit
TAATCGCTGTACATCTCTCTGAACCGT10.6711409395973155No Hit
TCTGCGATATGGTTTTTCAAGGGAGATGGGC10.6711409395973155No Hit
TCTTTGAAGAACTCTCGTGCTTGCGCTG10.6711409395973155No Hit
TATGGTTTTTCAAGGGAGATGGGCTATT10.6711409395973155No Hit
TTCCAATAGAACTCGTGAATCGCAGTGGGC10.6711409395973155No Hit
TCTGTCCATGTCGCAAACCCTCTTTCTCCT10.6711409395973155No Hit
TTGATCTTCCAACTTGACCTCCTCTGGTACT10.6711409395973155No Hit
TTTCTTTACCAAGTACCCCTGAACCTTC10.6711409395973155No Hit
AGGGGCAGAACTTTTTTCATTAGAGGACC10.6711409395973155No Hit
TAGGGGCAGAACTTTTTTCATTAGAGGACC10.6711409395973155No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCC10.6711409395973155No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGGT10.6711409395973155No Hit
TCTTTACCAAGTACCCCTGAACCTTCAGG10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)