FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.2997.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2997.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length23-33
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACATAAAATTACAAGATCGAGGCCCCC411.11111111111111No Hit
CCTGTGAAGAAACTGTGCCCGGA411.11111111111111No Hit
CCTGTGAAGAAACTGTGCCCGGAGAA38.333333333333332No Hit
TCCCAGACCGACGGCCCCTGTGAAGAAACT38.333333333333332No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGC25.555555555555555No Hit
ATTACATAAAATTACAAGATCGAGGCCCC25.555555555555555No Hit
CTGTGAAGAAACTGTGCCCGGAGAAA25.555555555555555No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAA25.555555555555555No Hit
CCTGTGAAGAAACTGTGCCCGGAGA25.555555555555555No Hit
TTGATACCTACCACCACTCCAATCCGT12.7777777777777777No Hit
TTACATAAAATTACAAGATCGAGGCCCCC12.7777777777777777No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAAAGC12.7777777777777777No Hit
CTGTGAAGAAACTGTGCCCGGAGA12.7777777777777777No Hit
GCCCCTGTGAAGAAACTGTGCCCGGAG12.7777777777777777No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAA12.7777777777777777No Hit
TAAAATTACAAGATCGAGGCCCCCACAT12.7777777777777777No Hit
TTACAAGATCGAGGCCCCCACATCTCACT12.7777777777777777No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGA12.7777777777777777No Hit
GCCCCTGTGAAGAAACTGTGCCCGGAGAAAGCT12.7777777777777777No Hit
CCCCTGTGAAGAAACTGTGCCCGGA12.7777777777777777No Hit
TTCGGAACCACCCTGCTGCGCCGTTGAA12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)