FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.2997.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.2997.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943
Sequences flagged as poor quality0
Sequence length19-35
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1155.918682449819866No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGT1075.50694801852805No Hit
TACTTAATTGTGAACTCATGCCGGGGAA713.654143077714874No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC603.0880082346886257No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC542.779207411219763No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA502.573340195573855No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG492.521873391662378No Hit
TAACTCTTCGTCCGACTCGACATCCATA371.9042717447246524No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT311.59547092125579No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC281.4410705095213587No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT271.3896037056098816No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG241.2352032938754505No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA241.2352032938754505No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT241.2352032938754505No Hit
TCACAGGGGACGTCGGTCTGGGACACTC221.1322696860524961No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC221.1322696860524961No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC211.0808028821410192No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC180.9264024704065877No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA170.8749356664951106No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT170.8749356664951106No Hit
TACTTAATTGTGAACTCATGCCGGGGA170.8749356664951106No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT160.8234688625836336No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT150.7720020586721564No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC140.7205352547606794No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC140.7205352547606794No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT140.7205352547606794No Hit
TTGGCTTTCGAAGGAGACGGATGTGTGCC130.6690684508492023No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG130.6690684508492023No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT120.6176016469377252No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG120.6176016469377252No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT110.5661348430262481No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT110.5661348430262481No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC110.5661348430262481No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC110.5661348430262481No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC110.5661348430262481No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG100.514668039114771No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC100.514668039114771No Hit
TACTTAATTGTGAATTCATGCCGGGGAA100.514668039114771No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC100.514668039114771No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT90.46320123520329387No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACTT90.46320123520329387No Hit
TGGGCACATCTGCACTGGTTGGATTGATA90.46320123520329387No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC90.46320123520329387No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG80.4117344312918168No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT80.4117344312918168No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC80.4117344312918168No Hit
TACTTAATTGTGAATTCATGCCGGGGA80.4117344312918168No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.4117344312918168No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCT70.3602676273803397No Hit
TTTGAAGAATTCTAGCACCACGATGAAAT70.3602676273803397No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT70.3602676273803397No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT70.3602676273803397No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT70.3602676273803397No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC70.3602676273803397No Hit
TCAATGGGGAGACTACTTTGAATGAAAAGTC60.3088008234688626No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC60.3088008234688626No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA60.3088008234688626No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG60.3088008234688626No Hit
TGAAACCGTGCACAATGTCTTCCAAGTTGC60.3088008234688626No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT60.3088008234688626No Hit
TAGATATGGGCACATCTGCACTGGTTGGA60.3088008234688626No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC60.3088008234688626No Hit
TGCTGGTAGATATGGGCACATCTGCACTG50.2573340195573855No Hit
TTAATTGTGGTGGTCTAGAACTAGGAATT50.2573340195573855No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT50.2573340195573855No Hit
TACAGGGTCAATTGTTGCGTAGTAATCTCC50.2573340195573855No Hit
TAGTCGGCTTCTTTCATAATTATTTGATGC40.2058672156459084No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT40.2058672156459084No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC40.2058672156459084No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.2058672156459084No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT40.2058672156459084No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAA40.2058672156459084No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAAT40.2058672156459084No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCT40.2058672156459084No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT40.2058672156459084No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGGG40.2058672156459084No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGA40.2058672156459084No Hit
TCGATGAAATAGTGGGGGCAGCAGACCT40.2058672156459084No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG40.2058672156459084No Hit
TGCACTTTGGCTCCGGATGCTTTCTC40.2058672156459084No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT40.2058672156459084No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG40.2058672156459084No Hit
TATGGGCACATCTGCACTGGTTGGATT40.2058672156459084No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG40.2058672156459084No Hit
TGAGTACAGTTTGAAGAATTCTAGCACC40.2058672156459084No Hit
TTGGTCCAGTGAGGCGTTTGAAATATGAGC40.2058672156459084No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT40.2058672156459084No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG40.2058672156459084No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT40.2058672156459084No Hit
TTCGTCCGACTCGACATCCATAATTGGCG40.2058672156459084No Hit
GAGTAATTGTTCTCCCGTATTTCTGGGGC40.2058672156459084No Hit
TATGAGCTTCAACGGCGCAGCAGGGTG40.2058672156459084No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA40.2058672156459084No Hit
TTGAAATATGAGCTTCAACGGCGCAGCAGGG30.1544004117344313No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTA30.1544004117344313No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGT30.1544004117344313No Hit
TAGATATGGGCACATCTGCACTGGTTGG30.1544004117344313No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGT30.1544004117344313No Hit
TGCTTCCAGTAGACTTCCTTCGGTTG30.1544004117344313No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCC30.1544004117344313No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTCT30.1544004117344313No Hit
TTCCTCTTCGCCTTCTTCATAGTTGTCGGA30.1544004117344313No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGAC30.1544004117344313No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGAC30.1544004117344313No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGG30.1544004117344313No Hit
TCGGTCTGGGACACTCTTGGAAGGTCA30.1544004117344313No Hit
TGCACTGGTTGGATTGATAGGAACAGTC30.1544004117344313No Hit
TCCAGATTCGCATGCTGACGCGACCACGCT30.1544004117344313No Hit
TGTGGGGGCCTCGATCTTGTAATTTTATGT30.1544004117344313No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA30.1544004117344313No Hit
ACAGTTTGAAGAATTCTAGCACCACAATG30.1544004117344313No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC30.1544004117344313No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC30.1544004117344313No Hit
TTCATCTCCAGATTCGCATGCTGACGCGACC30.1544004117344313No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG30.1544004117344313No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGG30.1544004117344313No Hit
CCCCGTCTGCGCACTGGATGAAACCGTGC30.1544004117344313No Hit
TACAGGGTCAATTGTTGCATAGTAATCTCC30.1544004117344313No Hit
TGTATAACGTGGACGTGGTCGTGGTGTT30.1544004117344313No Hit
TCGGACCCTTTACTACCTAATACATAGAAGT30.1544004117344313No Hit
ACGGACCCTTTACTACCTAATACATAGAAGT30.1544004117344313No Hit
TTAGATTGATAATTCCCTGAGGATGTTCCTCCC30.1544004117344313No Hit
TATGGGCACATCTGCACTGGTTGGA30.1544004117344313No Hit
TAAGCACACAGTAAGTTTATAAAAAGACTTG30.1544004117344313No Hit
TCAGCTCGTTGCAAGAACAGTACCGCATTGGC30.1544004117344313No Hit
TGTAAAACGTTTGCTTTGACAGGTATCCT30.1544004117344313No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTC30.1544004117344313No Hit
TACTTAATTGTGAACTCATGCCGGGGAATA30.1544004117344313No Hit
TTCGCATGCTGACGCGACCACGCTGCTGC30.1544004117344313No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG30.1544004117344313No Hit
TTCCAGTAGACTTCCTTCGGTTGACCTACA30.1544004117344313No Hit
ACTTGCACTTTGGCTCCGGATGCTTTCTC20.1029336078229542No Hit
TATATTATATTACAAGCTGTTCGTCCCGT20.1029336078229542No Hit
TACTTAATTGTGAATTCATGCCGGGGAAT20.1029336078229542No Hit
TGGTAGATATGGGCACATCTGCACTGGTTG20.1029336078229542No Hit
CTCGACATCCATAATTGGCGGTTTGCTG20.1029336078229542No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAT20.1029336078229542No Hit
GCTTGCTTCCAGTAGACTTCCTTCGGTT20.1029336078229542No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAAA20.1029336078229542No Hit
TAGTAATTGTTCTCCCGTATTTCTGGGGC20.1029336078229542No Hit
ACTTAATTGTGAACTCATGCCGGGGAA20.1029336078229542No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGG20.1029336078229542No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGCCT20.1029336078229542No Hit
TATGGGCACATCTGCACTGGTTGGATTG20.1029336078229542No Hit
TCTCAACAGTTGCCCCGTCTGCGCACT20.1029336078229542No Hit
TCACAGGGGCCGTCGGTCTGGGACACT20.1029336078229542No Hit
TAACTCTTCGTCCGACTCGACATCCAT20.1029336078229542No Hit
TGCTGGTAGATATGGGCACATCTGCACT20.1029336078229542No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAA20.1029336078229542No Hit
TTAGATTGATAATTCCCTGAGGATGTGA20.1029336078229542No Hit
TCACAGGGGACGTCGGTCTGGGACACTCT20.1029336078229542No Hit
TAGAGATGGGCACATCTGCACTGGTTGGAT20.1029336078229542No Hit
TCTCAACAGTTGCCCCGTCTGCGCACTG20.1029336078229542No Hit
TTCCAAATAGAGCACGGAATTGACGGCATA20.1029336078229542No Hit
TAGATATGGGCACATCTGCACTGGTTG20.1029336078229542No Hit
TCCAGTAGACTTCCTTCGGTTGACCTACA20.1029336078229542No Hit
TCGTGTATAACGTGGACGTGGTCGTGGTG20.1029336078229542No Hit
TCTCCAGATTCGCATGCTGACGCGACCACGCTGC20.1029336078229542No Hit
TCGATTTGGTCCAGTGAGGCGTTTGAAAT20.1029336078229542No Hit
TGCACTTGCACTTTGGCTCCGGATGCTTTC20.1029336078229542No Hit
TAAGACTGTAGTAAAGTTCTTTGTGGCGGT20.1029336078229542No Hit
TTGGTCCTTTTTCCAAATAGAGCACGGAAT20.1029336078229542No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCGT20.1029336078229542No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATCA20.1029336078229542No Hit
TAGATTGATAATTCCCTGAGGATGTGAAG20.1029336078229542No Hit
TTCATGCCGGGGAATAACTCTTCGTCCGAC20.1029336078229542No Hit
GACATCCATAATTGGCGGTTTGCTGGTA20.1029336078229542No Hit
TGGGGAGACCACTTTGAATGAAAAGTCAGA20.1029336078229542No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATT20.1029336078229542No Hit
TTGGCTTTCGAAGGAGACGGATGTGTGCCTC20.1029336078229542No Hit
TTCATGCCGGGGAATAACTCTTCGTCC20.1029336078229542No Hit
TCGTGTATAACGTGGACGTGGTCGTGG20.1029336078229542No Hit
ACGCTGCTGCTCGATGAAATAGTGGGG20.1029336078229542No Hit
ACTTAATTGTGAATTCATGCCGGGGA20.1029336078229542No Hit
TCGCACCCCTATTTCGACACTGCCATATCT20.1029336078229542No Hit
TTAGATTGATAATTCCCTGAGGATGTGAAG20.1029336078229542No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTGG20.1029336078229542No Hit
TGTACATACGGTCGGACCCTTTACTACCTA20.1029336078229542No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCT20.1029336078229542No Hit
AGGGACCCTTTACTACCTAATACATAGAAGT20.1029336078229542No Hit
TGCACTTTGGCTCCGGATGCTTTCTCC20.1029336078229542No Hit
TTCCGAACGAACTCGAGTAATTGTTCTCCC20.1029336078229542No Hit
TGCTGATCGGGAGTCAAATGCACTTGCAC20.1029336078229542No Hit
CGGACCCTTTACTACCTAATACATAGAGT20.1029336078229542No Hit
TGTGAACTCATGCCGGGGAATAACTCTTCGT20.1029336078229542No Hit
TTAAGCACACAGTAAGTTTATAAAAAGACT20.1029336078229542No Hit
GATGAAATAGTGGGGGCAGCAGACCTATA20.1029336078229542No Hit
TAGATCGTCTGGAACTTGGTAAGAGATA20.1029336078229542No Hit
TTGAAATATGAGTTTCAACGGCGCAGCA20.1029336078229542No Hit
ATACTTAATTGTGAACTCATGCCGGGGAA20.1029336078229542No Hit
TCTCCAGATTCGCATGCTGACGCGACCACG20.1029336078229542No Hit
TGTTCTGCGATGACGGTAAATGCGTTATT20.1029336078229542No Hit
TCCATAATTGGCGGTTTGCTGGTAGA20.1029336078229542No Hit
TTGGCGGTTTGCTGGTAGATATGGGCAC20.1029336078229542No Hit
TCAACAGTTGCCCCGTCTGCGCACTGGATG20.1029336078229542No Hit
TTGTTGAGTACAGTTTGAAGAATTCTAGC20.1029336078229542No Hit
TCAATGGGGAGACTACTTTGAATGAAAAGT20.1029336078229542No Hit
TTGGTATAGTCGGCTTCTTTCATAATTAT20.1029336078229542No Hit
GTTTGAAGAATTCTAGCACCACGATGAA20.1029336078229542No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTCC20.1029336078229542No Hit
TCCATTAGTGACCAAGCCGCCAATTCAAT20.1029336078229542No Hit
TCATCTCCAGATTCGCATGCTGACGCGACA20.1029336078229542No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGACAC20.1029336078229542No Hit
TTCTTGAAGTCCCGAGTTATCGCTTTCT20.1029336078229542No Hit
TAATTGTGAACTCATGCCGGGGAATAAC20.1029336078229542No Hit
TTAATTGTGAACTCATGCCGGGGAATAACT20.1029336078229542No Hit
TTCTTCACAGGGGACGTCGGTCTGGGACACT20.1029336078229542No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTCAAC20.1029336078229542No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCATA20.1029336078229542No Hit
TACAGGGTCAATTGTTGCATAGTAATCTC20.1029336078229542No Hit
TGTGCCGAACCGACGTAAGTTCTGTTGTGG20.1029336078229542No Hit
TACATAATTATGAACTTTGAAGTATATTA20.1029336078229542No Hit
TTCACAGGGGCCGTCGGTCTGGGACACT20.1029336078229542No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCC20.1029336078229542No Hit
TTGAAATATGAGCTTCAACGGCGCAGCA20.1029336078229542No Hit
TAGCAACCAAAGAAACATCTACAGACATGT20.1029336078229542No Hit
TAATTGTGAATTCATGCCGGGGAATAACT20.1029336078229542No Hit
TCTCCGGGCACAGTTTCTTCACAGGGGCC20.1029336078229542No Hit
TCCTTCGGTTGACCTACACATTTCGAGT20.1029336078229542No Hit
TCTTCACAGGGGACGTCGGTCTGGGACACTC20.1029336078229542No Hit
TGCCCCGTCTGCGCACTGGATGAAACCCTGA20.1029336078229542No Hit
TGATAGGAACAGTCGTGTATAACGTGGAC20.1029336078229542No Hit
TTATAGCAACCAAAGAAACATCTACAGAC20.1029336078229542No Hit
TTGTCGGAAGTTGTCGGAAGAAGAGCATC20.1029336078229542No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTTGACA20.1029336078229542No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGAG20.1029336078229542No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTC20.1029336078229542No Hit
ACTTGAACAATATCCTTATAGAGATT20.1029336078229542No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTT20.1029336078229542No Hit
GTACTCCTCGTGTATATATTGTTGTAGATC20.1029336078229542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)