FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.31032.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.31032.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length16-35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCAAGCCGCGCCTTACCACCCTAGCTACCTGC34.477611940298507No Hit
AAAAAGTAGCTCGGTGCGGGGTTCGAACCCG34.477611940298507No Hit
AAGCTGCCCGTCCCGTACTTTGTAACAGA22.9850746268656714No Hit
TAACGGAGAAAAAAAAGTAGCCCGGTGCGGG22.9850746268656714No Hit
ACGGAGAAAAAAAAGTAGCCCGGTGCGGG22.9850746268656714No Hit
TTACCAAGCCGCGCCTTACCACCCGAG22.9850746268656714No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGGT22.9850746268656714No Hit
TGAAAGGGTTGGCAGTTGGAAATGTAAC22.9850746268656714No Hit
TTGGCAGTTGGAAATGTAACGTCTGATTCGG22.9850746268656714No Hit
TACCAAGCCGCGCCTTACCACCCGAGCTAC22.9850746268656714No Hit
TCGGAAAAAAACTTGGCGTTTAGGTGTGG22.9850746268656714No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACAG22.9850746268656714No Hit
TGTTACCAAGCCGCGCCTTACCACCCTAGCAC22.9850746268656714No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACG11.4925373134328357No Hit
AGCTGCCCGTCCCGTACTTTGTAACTGAGA11.4925373134328357No Hit
ACCAAGCCGCGCCTTACCACCCTAGCTACC11.4925373134328357No Hit
TTAGTGTTACCAAGCCGCGCCTTACCACCC11.4925373134328357No Hit
AAAAAACAAGCTGCCCGTCCCGTACTTT11.4925373134328357No Hit
CCTTAGGGTTACCAAGCCGCGCCTTACCACCCG11.4925373134328357No Hit
TGTCAAAAAAAAAATCGGAAAAAAACTTGGC11.4925373134328357No Hit
TAAACAAGCTGCCCGTCCCG11.4925373134328357No Hit
TTACCAAGCCGCGCCTTACCACCCTAG11.4925373134328357No Hit
CAAGCTGCCCGTCCCATACTTTGTAACGG11.4925373134328357No Hit
CAAGCCGCGCCTTACCACCCGA11.4925373134328357No Hit
TGTAACGGAGAGAAAAAAAAGTAGCCCGGTGCGGG11.4925373134328357No Hit
GTTACCAAGCCGCGCCTTACCACCCTAGCTACCT11.4925373134328357No Hit
TACAAGCTGCCCGTCCCGTACTTTGTAACAGA11.4925373134328357No Hit
AAAAGTAGCTCGGTGCGGGGTTCGAACCC11.4925373134328357No Hit
TACTGAACAAGCTGCCCGTCCCGTACTTTGTAACG11.4925373134328357No Hit
GTTTTCTTGGCTGTAC11.4925373134328357No Hit
TTACAAGCTGCCCGTCCCGTACTTTGTAA11.4925373134328357No Hit
AAAAAAAAGTAGCTCGGTGCGGGGTTCGAAC11.4925373134328357No Hit
TAGACAAGCTGCCCGTCCCGTACTT11.4925373134328357No Hit
GCTGCCCGTCCCGTACTTTGTA11.4925373134328357No Hit
TTTGGCAGTTGGAAATGTAACGTCTGATT11.4925373134328357No Hit
ACCAAGCCGCGCCTTACCACCCTAGCTACCTGCA11.4925373134328357No Hit
TTACCAAGCCGCGCCTTACCACCCTAGCTACCTG11.4925373134328357No Hit
TACACAAGCTGCCCGTCCCGTACTTTGTAAC11.4925373134328357No Hit
TTAAGGTTACCAAGCCGCGCCTTACCACCC11.4925373134328357No Hit
AACAAGCTGCCCGTCCCGTACTTTGT11.4925373134328357No Hit
TCCTCAAATGACGTCTAGCGTCGGTCCTTG11.4925373134328357No Hit
TACCAAGCCGCGCCTTACCACCCTCGCTAC11.4925373134328357No Hit
TACCAAGCCGCGCCTTACCACCCG11.4925373134328357No Hit
ACCAAGCCGCGCCTTACCACCCTA11.4925373134328357No Hit
AAAAAAAGTAGCCCGGTGCGGG11.4925373134328357No Hit
TACCAAGCCGCGCCTTACCACCCGAGC11.4925373134328357No Hit
AACGGAGAAAAAAAAGTAGCCCGGTGCGGG11.4925373134328357No Hit
ACAAGCTGCCCGTCCCG11.4925373134328357No Hit
CAAGCCGCGCCTTACCACCCTAGCTACCTGC11.4925373134328357No Hit
TAAAACAAGCTGCCCGTCCCGTACT11.4925373134328357No Hit
TGCTGATGGTGAACAATGACGGGTTCTGG11.4925373134328357No Hit
AGCTGCCCGTCCCGTACTTTGTAAC11.4925373134328357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)