FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.31032.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.31032.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251
Sequences flagged as poor quality0
Sequence length15-35
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGTAAGGCGCGGCTTGGTAACC135.179282868525896No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACT83.187250996015936No Hit
TACAAAGTACGGGACGGGCAGCTTGTTAGG83.187250996015936No Hit
TAGGGTGGTAAGGCGCGGCTTGGTAACC72.788844621513944No Hit
CTCGGGTGGTAAGGCGCGGCTTGGTAACC62.3904382470119523No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTTTA62.3904382470119523No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAACC51.9920318725099602No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACTTTTT51.9920318725099602No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCCT51.9920318725099602No Hit
TTACAAAGTACGGGACGGGCAGCTTGTT41.593625498007968No Hit
TAGTGTGGTAAGGCGCGGCTTGGTAACC41.593625498007968No Hit
TTCGAACCCCGCACCGAGCTACTTTTTT41.593625498007968No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACC41.593625498007968No Hit
TAAGGCGCGGCTTGGTAACCCTAAGGTCAC41.593625498007968No Hit
TCGGGTGGTAAGGCGCGGCTTGGTAACCC41.593625498007968No Hit
TGGTAAGGCGCGGCTTGGTAACCCTAAGGT31.1952191235059761No Hit
CGCACCGGGCTACTTTTT31.1952191235059761No Hit
TCACGGGTTCGAACCCCACACCGGGCTACTTTTT31.1952191235059761No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTTTTT31.1952191235059761No Hit
GTAAGGCGCGGCTTGGTAACCCTAAGGTCACGGG31.1952191235059761No Hit
CGGGTTCGAACCCCGCACCGGGCTACT31.1952191235059761No Hit
AGCTCGGGTGGTAAGGCGCGGCTTGGTAACCC31.1952191235059761No Hit
TCACGGGTTCGAACCCCGCACCGGGC31.1952191235059761No Hit
TCACGGGTTCGAACCCCGCACCGGGCTAC31.1952191235059761No Hit
TTACAAAGTACGGGACGGGCAGCTTGTA31.1952191235059761No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACCTTT31.1952191235059761No Hit
TTCGAACCCCGCACCGAGCTACTTTTT20.796812749003984No Hit
TTCGAACCCCGCACCGAGCTACTTT20.796812749003984No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAAC20.796812749003984No Hit
CGTTACAAAGTACGGGACGGGCAGCTTGT20.796812749003984No Hit
TAGGGTGGTAAGACGCGGCTTGGTAACCC20.796812749003984No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTT20.796812749003984No Hit
TACAAAGTACGGGACGGGCAGCTTGTTAGGT20.796812749003984No Hit
CACGGGTTCGAACCCCGCACCGGGCTAC20.796812749003984No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACTTTT20.796812749003984No Hit
AAGGCGCGGCTTGGTAACCCTAAGGTCACG20.796812749003984No Hit
CGGGTTCGAACCCCGCACCGGGCTATTTT20.796812749003984No Hit
TCACGGGTTCGAACCCCACACCGGGCTACTT20.796812749003984No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACTTTTTT20.796812749003984No Hit
ACGGGTTCGAACCCCGCACCGGGCTACTTTGGAGA20.796812749003984No Hit
CGTTACAAAGTACGGGACGGGCAGCTTG20.796812749003984No Hit
TTGGTAACCCTAAGGTCACGGGTTCGAACCC20.796812749003984No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTTT20.796812749003984No Hit
ACGGGTTCGAACCCCGCACCGGGCC20.796812749003984No Hit
GCTCGGGTGGTAAGGCGCGGCTTGGTA20.796812749003984No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCC20.796812749003984No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTTAAA20.796812749003984No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACTTTTGA20.796812749003984No Hit
TCACGGGTTCGAACCCCGCACCGGGCTA20.796812749003984No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTA20.796812749003984No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTTTAA10.398406374501992No Hit
ACGGGTTCGAACCCCGCACCGGGC10.398406374501992No Hit
CGGGTTCGAACCCCGCACCGGGCTACTTTTTTTA10.398406374501992No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCCT10.398406374501992No Hit
AGGTCATGGGTTCGAACCCCGCACCGGGCTAC10.398406374501992No Hit
TGTCACGGGTTCGAACCCCGCACCGGGC10.398406374501992No Hit
CACGGGTTCAAACCCCGCACCGGGCTACTTT10.398406374501992No Hit
CGCACCGGGCTACTT10.398406374501992No Hit
TAGCTAGGGTGGTAAGGCGCGGCTTGGTAACC10.398406374501992No Hit
CACGGGTTCGAACCCCGCACCGGGCTACT10.398406374501992No Hit
TCGGGTGGTAAGGCGCGGCTTGGTA10.398406374501992No Hit
TACAAAGTACGGGACGGGCAGCTTGTATT10.398406374501992No Hit
TGCTAGGGTGGTAAGGCGCGGCTTGGTA10.398406374501992No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAA10.398406374501992No Hit
ACGGGTTCGAACCCCACACCGGGCTAC10.398406374501992No Hit
CACGGGTTCGAACCCCACACCGGGCTACT10.398406374501992No Hit
CACGGGTTCGAACCCCGCACCGGGCT10.398406374501992No Hit
AGGAGGTCACGGGTT10.398406374501992No Hit
TACAAAGTACGGGACGGGCAGCTTGTTGTT10.398406374501992No Hit
AAGGCGCGGCTTGGTAACCCTAAGGTCAC10.398406374501992No Hit
TCACGGGTTCGAACCCCGCACCGGGCTACCTT10.398406374501992No Hit
CACGGGTTCGTACCCCGCACCGGGCTACT10.398406374501992No Hit
CGGGTTCGAACCCCGCACCGGGC10.398406374501992No Hit
CCGCACCGGGCTACTTTTTTTA10.398406374501992No Hit
TTTTTCTCCGTTACAAAGTACGGGACGGGCAGC10.398406374501992No Hit
AAGTACGGGACGGGCAG10.398406374501992No Hit
CGTTACAAAGTACGGGACGGGCAGCTAG10.398406374501992No Hit
AAAATTCGAGCAAACCGGGTCTGTAGAAG10.398406374501992No Hit
GTCACGGGTTCGAACCCCACACCGGGC10.398406374501992No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTTTTA10.398406374501992No Hit
GTCACGGGTTCGAACCCCGCACCGGGCTATCTCAA10.398406374501992No Hit
GTAAGGCGCGGCTTGGTAACCCTAAGGTCACGG10.398406374501992No Hit
GTCACGGGTTCGAACCCCGCACCGGGC10.398406374501992No Hit
CGGGTGGTAAGGCGCGGCTTGGT10.398406374501992No Hit
CGCACCGGGCTACTTTTTTTTCTC10.398406374501992No Hit
TCGAACCCCGCACCGGG10.398406374501992No Hit
GCGGCTTGGTAACCCTAAGGTCACGG10.398406374501992No Hit
TACGGGTTCGAACCCCGCACCGGGCTACT10.398406374501992No Hit
GGTGGTAAGGCGCGGCTTGGTAACCCTAAAAA10.398406374501992No Hit
TAGCTCGGGTGGTAAGGCGCGGCTTGGTAACCCTA10.398406374501992No Hit
AAGGTCACGGGTTCGAACCCCACACCGGGCT10.398406374501992No Hit
ATTCGAGCAAACCGGGTCTGTAGAAGATG10.398406374501992No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTT10.398406374501992No Hit
TTACAAAGTACGGGACGGGCAGCTTGT10.398406374501992No Hit
CACGGGTTCGAACCCCGCACCGGGCTA10.398406374501992No Hit
TCGCGGGTTCGAACCCCGCACCGGGCTACTTTTT10.398406374501992No Hit
AGCTCGGGTGGTAAGGCGCGGCTTGGTAACC10.398406374501992No Hit
CGGGTGGTAAGGCGCGGCTTGGTAACC10.398406374501992No Hit
ACGGGTTCGAACCCCGCACCGGGCTACTTTTTT10.398406374501992No Hit
CGGGTTCGAACCCCGCACCGGGCTATTTTT10.398406374501992No Hit
ATTAGTAAGGCGCGGCTTGGTAACCCT10.398406374501992No Hit
CACGGGTTCGAACCCCGCACCGGGCTACTTTT10.398406374501992No Hit
GGTTCGAACCCCGCACCG10.398406374501992No Hit
AGGGTGGTAAGGCGCGGCTTGGTAACC10.398406374501992No Hit
ACGGGTTCGAACCCCACACCGGGCT10.398406374501992No Hit
ACACGGGTTCGAACCCCGCACCGGGCTACTTAAA10.398406374501992No Hit
GCAAACCGGGTCTGTAGAAGATGTGAGAAGAC10.398406374501992No Hit
TTTCTCCGTTACAAAGTACGGGACGGGCAGCT10.398406374501992No Hit
CACGGGTTCGAACCCCGCAACGGGCTAC10.398406374501992No Hit
TCACGGGTTCGAACCCCGCACCGGGCTAAA10.398406374501992No Hit
ACGGGTTCGAACCCTGCACCGGGCTACTTTTT10.398406374501992No Hit
ACGGGTTCGAACCCCGCACCGGGCATT10.398406374501992No Hit
CCGCACCGGGCTACTTTT10.398406374501992No Hit
TAACGGGTTCGAACCCCGCACCGGGCTAC10.398406374501992No Hit
TGTGGTAAGGCGCGGCTTGGTAACCCT10.398406374501992No Hit
TCGAGTGGTAAGGCGCGGCTTGGTAACC10.398406374501992No Hit
ACGGGTTCGAACCCCGCACCGGGCTACTT10.398406374501992No Hit
TCACGGGTTCGAACCCCACACCGGGCTA10.398406374501992No Hit
AGGTCACGAGTTCGAACCCCGCACCGGGCTACT10.398406374501992No Hit
TAGGGTGGTAGGGCGCGGCTTGGTAACC10.398406374501992No Hit
ACGGGTTCGAACCCCGCACCGGGCTAC10.398406374501992No Hit
TACAAAGTACGGGACGGGCAGCTTGTGTAAA10.398406374501992No Hit
GTAAGGCGCGGCTTGGTAACCCTAAGGTCA10.398406374501992No Hit
CGCACCGGGCTACTTTTTTT10.398406374501992No Hit
GTAGCTCGGGTGGTAAGGCGCGGCTTGGTAAC10.398406374501992No Hit
GGTCACGGGTTCGAACCCCACACCGGGCT10.398406374501992No Hit
AAGGCGCGGCTTGGTAACCCTAAGGTCACGGGTT10.398406374501992No Hit
GAGCAAACCGGGTCTGTAGAA10.398406374501992No Hit
CCGCACCGGGCTACTTTTT10.398406374501992No Hit
TACAAAGTACGGGACGGGCAGCTTGTGT10.398406374501992No Hit
AAGGCGCGGCTTGGTAACCCTGAGGTCACGGGT10.398406374501992No Hit
TTACAAAGTACGGGACGGGCAGCTTGTC10.398406374501992No Hit
CTCGGGTGGTAAGGCGCGGCT10.398406374501992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)