FastQCFastQC Report
Mon 30 Nov 2020
S33.15-35nt.3449.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.3449.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291
Sequences flagged as poor quality0
Sequence length15-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGACGTCCCCCCTTAACATTACTGCCCT7826.804123711340207No Hit
TGTGACGTCTCCCCTTAACATTACTGCCCT165.498281786941581No Hit
GGTGCTTAGGTCCTCCTGTTCTAACTAACT155.154639175257731No Hit
TCCCAGAGGTGACTTGCCTGAACATGTT82.7491408934707904No Hit
TAACATTACTGCCCTTAAGAGTTCTTCCGG72.405498281786942No Hit
TTAGAATCGTAGTCTGGGGGGAACAGCCT72.405498281786942No Hit
TTTGAATCCACACCTATCATAACCTTGCCC62.0618556701030926No Hit
TACTCAAGCATAGATCCAAGGGCAATCTCTT51.718213058419244No Hit
TCCTTAATTCTTTCATGGTCCATACCGGGT51.718213058419244No Hit
TTACTGACTCCCATCCCCAAAAAAGGCACC41.3745704467353952No Hit
TTAGAAAAGCAACACTGGATTCTCGAACTGT41.3745704467353952No Hit
TAGGTGACATGACGTGTGGTCTACGTGG41.3745704467353952No Hit
TCAGGACTGGGTACCCGAAATGCTTCATA41.3745704467353952No Hit
TTGGATATCGTTGACCCTCCAAAGTCCAC31.0309278350515463No Hit
TTCTCTACTTTCAGGACTGGGTACCCGAA31.0309278350515463No Hit
TTTGCACAGTGAATCCACGTCCACTACCCAG31.0309278350515463No Hit
TACTGACTCCCATCCCCAAAAAAGGCACCA31.0309278350515463No Hit
TGAACATGTTGTCCATATCCTCCACCCCT31.0309278350515463No Hit
TATCATAACCTTGCCCCCAATTGCTCAGT31.0309278350515463No Hit
CCTTGCCCCCAATTGCTCAGTTTGTGCGAG20.6872852233676976No Hit
TATTACATCGTCCAATGTGACGTCCCCCCT20.6872852233676976No Hit
TATAAAATCCAGTAGAGCTTGAGGGTGAAC20.6872852233676976No Hit
TCCACGTCCACTACCCAGCTTTAGTTCACAC20.6872852233676976No Hit
TCTCTACTTTCAGGACTGGGTACCCGAAA20.6872852233676976No Hit
TCCATATCCTCCACCCCTTCAGGAAGTAT20.6872852233676976No Hit
ACAACTTCTCTACTTTCAGGACTGGGTACCC20.6872852233676976No Hit
TAATTGGGGGGAGGAGG20.6872852233676976No Hit
TCTGAACATGGACATCTGATAGCTCGGCT20.6872852233676976No Hit
TTAGACCTTACACGTCACTCACTAGGAGATG20.6872852233676976No Hit
GTGTGGAGCTTACAC20.6872852233676976No Hit
TTTTGACTCCGTGACCTCGCTATCAGTCA20.6872852233676976No Hit
TAACATTACTGCCCTTAAGAGTTCTTCCG20.6872852233676976No Hit
TAGGTGACATGACGTGTTGTCTACGTGGA20.6872852233676976No Hit
TCCTTAATTCTCTCATGGTCCATACCGGGTT20.6872852233676976No Hit
TAGGTGACATGACGTGTTGTCTACG20.6872852233676976No Hit
TGTGACGTCTCCCCTTAACATTACTGCCC20.6872852233676976No Hit
TAAGGCTCCATGGTTCTTAAACTACGAACG20.6872852233676976No Hit
TGAACATGGACATCTGATAGCTCGGCTGCT20.6872852233676976No Hit
TCGAACTGTATGTTCCAAATTTGGCAAGGA20.6872852233676976No Hit
TTGTGCTCTCCTTGACTGTCG20.6872852233676976No Hit
TGTATGTTCCAAATTTGGCAAGGACTCAAT10.3436426116838488No Hit
TGTGACGTCCCCCCTTAACATTACTGCCCTT10.3436426116838488No Hit
TCTCCTTGACTGTCGTATTTATTCCCATCT10.3436426116838488No Hit
TAGAGTTCAGCAAATCATTAGAGTCAGGC10.3436426116838488No Hit
GGACTCCTCTGCATA10.3436426116838488No Hit
TATAAAATCCAGGAGAGCTTGAGGGTGAAC10.3436426116838488No Hit
TTATGGTTAGAATCGTAGTCTGGGGGGA10.3436426116838488No Hit
TCATAACCCCAAGAATCTCCAGATAGATC10.3436426116838488No Hit
GCCTGAACATGTTGTCCATATCCTCCACCC10.3436426116838488No Hit
TCAGGACTGGGTACCCGAAATGCTTCATAAC10.3436426116838488No Hit
TCCCCCCTTAACATTACTGCCCTTAAGAGT10.3436426116838488No Hit
TTAAATCAGGATCTCTACTTTTTATCAACCT10.3436426116838488No Hit
TCAGACAAAATTCTCTCTTAACTACACCCAT10.3436426116838488No Hit
GCATTACTCGTGTTTCCTCCTTCCCTCCGT10.3436426116838488No Hit
TACTCGTGTTTCCTCCTTCCCTCCGTAAT10.3436426116838488No Hit
TGATAGCTCGGCTGCTAACCTAATAGCACC10.3436426116838488No Hit
TCTCTACTTTCAGGACTGGGTACCCGA10.3436426116838488No Hit
TGGTTTATGGTTAGAATCGTAGTCTGGG10.3436426116838488No Hit
TTCGTCTTCCATGATTCGTCTTCCATGTT10.3436426116838488No Hit
TAAAATCCAGTAGAGCTTGAGGGTGAACC10.3436426116838488No Hit
TCATCATAATCGCTCCCTTCTCGTCCTTGT10.3436426116838488No Hit
AGAGCTTTTACCCCAACCGATTTCTCTAGC10.3436426116838488No Hit
TCATAACCTTGCCCCCAATTGCTCAGTT10.3436426116838488No Hit
TGTCTAATCTCGCCAGTCCCTCTGTCATA10.3436426116838488No Hit
TAGGTCCTCCTGTTCTAACTAACTTTGTGCT10.3436426116838488No Hit
TCCTTGACTGTCGTTTTTATTCCCATCTT10.3436426116838488No Hit
TCTTACACGCTCTCTTAGTACCCTGGCTTA10.3436426116838488No Hit
TGCTTCATAACCCCAAGAATCTCCAGATAGAT10.3436426116838488No Hit
TGTGACGTCCCCCCTTAACATTACTGCC10.3436426116838488No Hit
GCTTAGGTCCTCCTGTTCTAACTAACT10.3436426116838488No Hit
TAATTCTTTCATGGTCCATACCGGGTTC10.3436426116838488No Hit
TCTAATAATCTTTTTGACTCCGTGACCTCGC10.3436426116838488No Hit
TGTCATAGTGTTGAGGGTGCTGATCATAG10.3436426116838488No Hit
TTTGACTCCGTGACCTCGCTATCAGTCATAG10.3436426116838488No Hit
TACTCGTGTTTCCTCCTTCCCTCCGTA10.3436426116838488No Hit
TCATAACCTTGCCCCCAATTGCTCAGTTT10.3436426116838488No Hit
TGCTTCATAACCCCAAGAATCTCCAGA10.3436426116838488No Hit
TTCGTCTTCCATGTTTAGGTCCTCCTGTT10.3436426116838488No Hit
TGTGACGTCCCCCCTTAACATTACTACCCT10.3436426116838488No Hit
TAATTGGGGGGAGGAGGCTTATCATAAAGC10.3436426116838488No Hit
CCCCTTAACATTACTGCC10.3436426116838488No Hit
TGTGACGTCCCCCCTTAACATTACTGCCC10.3436426116838488No Hit
AACATTACTGCCCTTAAGAGTTCTTCCGG10.3436426116838488No Hit
TGCTCCTCTGTGCACTGGTCTACAGGAATT10.3436426116838488No Hit
TTAATTCTCTCATGGTCCATACCGGGTT10.3436426116838488No Hit
TTCTCTCATGGTCCATACCGGGTTCTATTCT10.3436426116838488No Hit
TCCGGTAGGCCATCCAATAAATATTCCT10.3436426116838488No Hit
TAGAGCATACGGATAGCAGGATTGGTTAC10.3436426116838488No Hit
TTAGGGTGGTTATCACTAGATGATGTGTT10.3436426116838488No Hit
TGCCTGAACATGTTGTCCATATCCTCCACC10.3436426116838488No Hit
TGTATATCTTGAATGAAGTCCCAGAGGT10.3436426116838488No Hit
TCCTAAGTTGAGGAATGCCTTGATGTGCCC10.3436426116838488No Hit
TGAATTACTGACTCCCATCCCCAAAAAAGG10.3436426116838488No Hit
TCTGATAGCTCGGCTGCTAACCTAATAGCACCTAT10.3436426116838488No Hit
TTCTCTACTTTCAGGACTGGGTACCCGAAAT10.3436426116838488No Hit
TTATCTTACACGCTCTCTTAGTACCCTGGCCC10.3436426116838488No Hit
GACGTCCCCCCTTAACATTACTGCCC10.3436426116838488No Hit
TTTATGGTTAGAATCGTAGTCTGGGGGG10.3436426116838488No Hit
GTGCTTAGGTCCTCCTGTTCTAACTAACT10.3436426116838488No Hit
TACTGACTCCCATCCCCAAAAAAGGCACC10.3436426116838488No Hit
GGTGCTTAGGTCCTCCTGTTCTAACTAACTT10.3436426116838488No Hit
TTCTATTCTAAAACAAAGGGTTTGTACATC10.3436426116838488No Hit
TTCAGGACTGGGTACCCGAAATGCTTCATAA10.3436426116838488No Hit
TGCATAGATTGGAGAGCTGTGATGCCCC10.3436426116838488No Hit
TGACTTGCCTGAACATGTTGTCCATATCCT10.3436426116838488No Hit
CCTGCGATTGTTGCAACCTTGTGGTTGTC10.3436426116838488No Hit
GTGACGTCCCCCCTTAACATTACTGCCCT10.3436426116838488No Hit
ATTACATCGTCCAATGTGACGTCCCCCCT10.3436426116838488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)