FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.3449.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.3449.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length16-35
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGGAGAGCACAAAGTTAGTTAGAACA315.0No Hit
AAGGGCAGTAATGTTAAGGGGGGACGT315.0No Hit
CATGAGAGAATTAAGGAATATTTATTGGATGGCCT210.0No Hit
AAGGGCAGTAATGTTAAGGGGGGACGTC210.0No Hit
TATTGGATGGCCTACCGGAAGAACTCCT210.0No Hit
ATGGCCTACCGGAAGAACTCCT15.0No Hit
GCTCCACGTAGACAACACGTCATGTCACC15.0No Hit
AATATTTATTGGATGGCCTACCGGAAGAACTCCTA15.0No Hit
GAAGAACTCCTAAGGGC15.0No Hit
AAGGAGAGCACAAGGTTAGTTAGAACAGGAGGACC15.0No Hit
TTAAGGAATATTTATTGGATGGCCTACCGGAAGAA15.0No Hit
ACAATCGCAGGAAAAGAGTGTGAACTAAA15.0No Hit
CTACCGGAAGAACTCC15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)