FastQCFastQC Report
Mon 16 Nov 2020
S33.15-35nt.3452.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.15-35nt.3452.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031
Sequences flagged as poor quality0
Sequence length24-34
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCTCGGTACTGACTCGACAAGCGCC12412.027158098933075No Hit
TCTATATTGTCGAATAACCCACCTCTTGG494.75266731328807No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCT393.7827352085354025No Hit
TTAACGACCCGCTCTGTTCCATATCGATA343.2977691561590685No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCT302.909796314258002No Hit
TATTGTCGAATAACCCACCTCTTGG282.7158098933074686No Hit
TAACGACCCGCTCTGTTCCATATCGATAT252.424830261881668No Hit
CACGTCGTGTACGACGCGAGACCGCGGAG201.939864209505335No Hit
TCGGTACTGACTCGACAAGCGCCTCGTAC191.842870999030068No Hit
TTAACGACCCGCTCTGTTCCATATCGATAT171.6488845780795343No Hit
TATACGTCGTGTACGACGCGAGACCGCGGA171.6488845780795343No Hit
TGGGCTCGGTACTGACTCGACAAGCGCCT171.6488845780795343No Hit
TTAGATGGACCCGGTATAGGATTTTCGCTC161.5518913676042678No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTC161.5518913676042678No Hit
TAACACAAGCCCGATCGCTCCGATACATG141.3579049466537343No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTC141.3579049466537343No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGAC131.2609117361784674No Hit
TGGGCTCGGTACTGACTCGACAAGCGCC131.2609117361784674No Hit
TCGGTACTGACTCGACAAGCGCCTCGTACT121.1639185257032008No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGT100.9699321047526674No Hit
AAAAGTCACCGTCGTCCTCATGACTGCCT100.9699321047526674No Hit
TACAATTTCTGCATCGTTATAGCACTGAT80.7759456838021339No Hit
TCTACATTGTCGAATAACCCACCTCTTGGT80.7759456838021339No Hit
TACTGACTCGACAAGCGCCTCGTACTTGT70.6789524733268671No Hit
TCATCTTCTTGTTCGGAAAAGTCGCTGGCT70.6789524733268671No Hit
TATTGTCGAATAACCCACCTCTTGGTG70.6789524733268671No Hit
TACTTGGATTTGTGGGATGATGGCATCGC60.5819592628516004No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAG60.5819592628516004No Hit
TAACGACCCGCTCTGTTCCATATCGATA60.5819592628516004No Hit
TCTATATTGTCGAATAACCCACCTCTTGGT60.5819592628516004No Hit
TGCCGAAGCCATAAAAGGACCGATTCGGGC50.4849660523763337No Hit
TAGATGGACCCGGTATAGGATTTTCGCTC50.4849660523763337No Hit
TATTGTCGAATAACCCACCTCTTGGT50.4849660523763337No Hit
CACGACGCATTTTCGTAGTTCTCCCCAAGC50.4849660523763337No Hit
TCTGAGAAAAAGTCACCGTCGTCCTCATGAC40.38797284190106696No Hit
TACACGTCGTGTACGACGCGAGACCGCGGA40.38797284190106696No Hit
TTAGATGGACCCGGTATAGGATTTTCGCT40.38797284190106696No Hit
TTCCAACGTCTCGTTCCATCGGATC40.38797284190106696No Hit
TAGACGTTAACGGCGCTGAGGGGTGTTG40.38797284190106696No Hit
CGCGAGACCGCGGAGGCACTCTTTGAAGG40.38797284190106696No Hit
TATTCACTGGCTTCCGCTTCAAAACTCCT40.38797284190106696No Hit
TTGGGCTCGGTACTGACTCGACAAGCGCCC40.38797284190106696No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCTC40.38797284190106696No Hit
TCCCAAACTTCGACTCGGACCAGTGCCGG40.38797284190106696No Hit
TAGGGAATTTTTGAATGGCTGCTATTTT40.38797284190106696No Hit
TATTTTTTTTGGGCGGATTCTTTCGGGG40.38797284190106696No Hit
TGAATTGCCGTGGCTCGGAAGTTGGGCTCT40.38797284190106696No Hit
TCCGAATCGATATTGTCGAATAACCCACCT40.38797284190106696No Hit
TTTGTTAACTGGCCGACTTCCCCCGCCTGA30.2909796314258002No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGCC30.2909796314258002No Hit
ATCTATATTGTCGAATAACCCACCTCTTG30.2909796314258002No Hit
TTGAATTGCCGTGGCTCGGAAGTTGGGCTC30.2909796314258002No Hit
TCGAGAAATTGGGACACTGTAATATTGGGT30.2909796314258002No Hit
TTTACGGTAGTAGCCTAGTAATCCGAGAAA30.2909796314258002No Hit
TGAGGACCAGGGGCTGTGTGAGGGCCTAA30.2909796314258002No Hit
TTGGGGATCCCGTAAGAAATACACGTCGTG30.2909796314258002No Hit
TATATTGTCGAATAACCCACCTCTTGG30.2909796314258002No Hit
TACTGACTCGACAAGCGCCTCGTACT30.2909796314258002No Hit
TAACGACCCGCTCTGTTCCATATCGATATT30.2909796314258002No Hit
TTTGTCCGGAGGCATCTATTTTTTTGGGA30.2909796314258002No Hit
TCTTTTGTTAACTGGCCGACTTCCCCCGC30.2909796314258002No Hit
TAACAGGAGTACGGCCCACAGTAGCGTC30.2909796314258002No Hit
TTGTTAACTGGCCGACTTCCCCCGCCT30.2909796314258002No Hit
TTCTACTTGGATTTGTGGGATGATGGCATC30.2909796314258002No Hit
TCTTCTTGTTCGGAAAAGTCGCTGGCTTCGT30.2909796314258002No Hit
TCGGAAAAGTCGCTGGCTTCGTATTCACT30.2909796314258002No Hit
TCGGTAGTTAATTCGAAGGGTTTCGAGAA30.2909796314258002No Hit
TGATGGCAGGAGATTGAACGTTTGTTTCG30.2909796314258002No Hit
TACTGGAAGTGCGGAGAACTTTAGTCCCA30.2909796314258002No Hit
TGCAATATAGACGTTAACGGCGCTGAGGG20.19398642095053348No Hit
TCGATTAACGAACTCCACACTTCTTTGGG20.19398642095053348No Hit
TGCACAGACGCGGTGTTTAGTCTCTGAAA20.19398642095053348No Hit
TTTGTTAACTGGCCGACTTCCCCCGCC20.19398642095053348No Hit
GGATGAAGACGCAGCGGCATGTTGAACTG20.19398642095053348No Hit
TTGGGCGGATTCTTTCGGGGGTGACAATA20.19398642095053348No Hit
TTTGTTGTAACATTTTTTGTATTTGATTGT20.19398642095053348No Hit
TGAGAAAAAGTCACCGTCGTCCTCATGA20.19398642095053348No Hit
TCACTGGCTTCCGCTTCAAAACTCCTCT20.19398642095053348No Hit
TCGATATTGTCGAATAACCCACCTCTTGG20.19398642095053348No Hit
TTAGAGTTTCAAATGCTGTTGGTTCTGCT20.19398642095053348No Hit
TACATTGTCGAATAACCCACCTCTTGGT20.19398642095053348No Hit
TTAACGACCCGCTCTGTTCCATATCGATAA20.19398642095053348No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTGC20.19398642095053348No Hit
TTCGGTGTCCACACGAGAAACAGAAACGC20.19398642095053348No Hit
ACCACCCAAAGAGATGAGGACCAGGGGCTGT20.19398642095053348No Hit
TCAAAACTCCCTCTTCGAACGTAACACAAGC20.19398642095053348No Hit
TCACCGTCGTCCTCATGACTGCCTCCTGC20.19398642095053348No Hit
TAAAAGGACCGATTCGGGCAGTGAGTGTCT20.19398642095053348No Hit
TCGTGGATGACGTGGACGTGGTCGTTGTG20.19398642095053348No Hit
TGCCTCCTGCCACCAATCCTTCTTGTTTT20.19398642095053348No Hit
TTGTTCGGAAAAGTCGCTGGCTTCGTATT20.19398642095053348No Hit
TGCCACGACGCATTTTCGTAGTTCTCCCC20.19398642095053348No Hit
CATTGTCGAATAACCCACCTCTTGGT20.19398642095053348No Hit
TCCGGCGCGCTAGAGTGGCTGCATGAAGGAA20.19398642095053348No Hit
TTGGTATGATCACCTCTGAGTATGGGGCT20.19398642095053348No Hit
CTTGTTCGGAAAAGTCGCTGGCTTCGTATT20.19398642095053348No Hit
TGGCTTCCGCTTCAAAACTCCTCTCGAT20.19398642095053348No Hit
TATAGACGTTAACGGCGCTGAGGGGTGTT20.19398642095053348No Hit
TTAACGACCCGCTCTGTTCCATATCGATATT20.19398642095053348No Hit
TCACACACACTACTGATACTATCGGTCTCA20.19398642095053348No Hit
TTCTTGTTCGGAAAAGTCGCTGGCTTCGTAT20.19398642095053348No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAC20.19398642095053348No Hit
TATACGTCGTGTACGACGCGAGACCGCGGAA20.19398642095053348No Hit
TTTTGTTAACTGGCCGACTTCCCCCGCCT20.19398642095053348No Hit
TCATGACTGCCTCCTGCCACCAATCCTTC20.19398642095053348No Hit
TTAGATGGACCCGGTATAGGATTTTCGGTC20.19398642095053348No Hit
TATTAATGAGACCGCGGAGGACTATCTC20.19398642095053348No Hit
TTCGACTGTTAGGTGGGCAAAATTTGGGA20.19398642095053348No Hit
TTCAAATGCTGTTGGTTCTGCTTGCGGCTC20.19398642095053348No Hit
TTCTAACAAACTGACGGACACAAACCCTG20.19398642095053348No Hit
TTAACGACCCGCTCTGTTCCATATCGAT20.19398642095053348No Hit
AACGACCCGCTCTGTTCCATATCGATATTGTC20.19398642095053348No Hit
TGCGGCTCATTCCAAAGAAGAATTCTTTT20.19398642095053348No Hit
TCCTCATGACTGCCTCCTGCCACCAATCC20.19398642095053348No Hit
TATCGCCTATTGTTTTTTCGTTTAGTTTC20.19398642095053348No Hit
CTCCCTCTTCGAACGTAACACAAGCCCGATC20.19398642095053348No Hit
TTACGGTAGTAGCCTAGTAATCCGAGAAAT20.19398642095053348No Hit
TCTTGCTTTCGACGCGCTAGAGTGGCTG20.19398642095053348No Hit
TCGGATCCTATTGCATATGCGGATGCATC20.19398642095053348No Hit
TGCGAGACCGCGGAGGCACTCTTTGAAGCAG20.19398642095053348No Hit
TCGTACTTGTCGATTAACGAACTCCACACT20.19398642095053348No Hit
TTATTAATGAGACCGCGGAGGACTATCTC20.19398642095053348No Hit
TCGTCCTCATGACTGCCTCCTGCCACCAATC20.19398642095053348No Hit
TATATTGTCGAATAACCCACCTCTTGGT20.19398642095053348No Hit
TTAATTCGAAGGGTTTCGAGAAATTGGGAC20.19398642095053348No Hit
TCCCTCTTCGAACGTAACACAAGCCCGATC20.19398642095053348No Hit
TAACACAAGCCCGATCGCTCCGATACATGG20.19398642095053348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)