FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.16463.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.16463.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length11-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTACGGCCGCCGATTTGTTTCTCTTGGC119.401709401709402No Hit
TATTACGGCCGCCGATTTGTTTCTCTTGG97.6923076923076925No Hit
TAATTAGGGGAAAGCTGAATTTTGGATATGTGATAGAACATGTTAAGAGACAC43.418803418803419No Hit
TCCACCCCCCGCCTGCCTATATGA32.564102564102564No Hit
AGGTTATTTCCACCCCCCGCCTGCCTATATGAGTTGAACCC32.564102564102564No Hit
CCCCGCCTGCCTATATGAGTTGAACA32.564102564102564No Hit
AATTAGGGGAAAGCTGAATTTTGGATATGTGATAGAACATGTT32.564102564102564No Hit
TTTTTTCCACCCCCCGCCTGCCTATATG32.564102564102564No Hit
TTCCACCCCCCTCCTGCCTATATGAGTTGAAC32.564102564102564No Hit
GACTGGACTATTGGTCATTAAATATCCTCTGCCTGACCAGCAGTGGTGGCGCTCGGG32.564102564102564No Hit
TTATTACGGCCGCCGATTTGTTTCTCTTG21.7094017094017095No Hit
CTCATGTGGGCAGGTGGGGGGGAGT21.7094017094017095No Hit
TTCCACCCCCCTCCTGCCTATATGAGTTGAA21.7094017094017095No Hit
TTCCACCCCCCTCCTGCCTATATGAGTT21.7094017094017095No Hit
TTTCCACCCCCCGCCTGCCTATATGA21.7094017094017095No Hit
TTCCACCCCCCTCCTGCCTATATGAGTTG21.7094017094017095No Hit
TTTTTTCCACCCCCCGCCTGCCTATATGA21.7094017094017095No Hit
ATTTATTACGGCCGCCGATTTGTTTCTC21.7094017094017095No Hit
TTATTTCCACCCCCCGCCTGCCTATATGAGTT21.7094017094017095No Hit
TTACGGCCGCCGATTTGTTTCTCTTGG21.7094017094017095No Hit
TCCACCCCCCGCCTGCCTATGTGAGTTGAAC21.7094017094017095No Hit
TTTCCACCCCCCTCCTGCCTATATGAGT10.8547008547008548No Hit
TTATTTCCACCCCCCGCCTGCCTATATGAGT10.8547008547008548No Hit
TGCCACTTGGGAGCGCTAGTAGTAAAAACAGC10.8547008547008548No Hit
TTCCACCCCCCGCCTGCCTATATGAGTTGAACCC10.8547008547008548No Hit
ATTTCCACCCCCCGCCTGC10.8547008547008548No Hit
GTTTCCACCCCCCGCCTGCCT10.8547008547008548No Hit
TAAATAATACCCCCCAAGATTACATAAACCT10.8547008547008548No Hit
TGAAAGTTCAATTTATTTATTACGGCCGCC10.8547008547008548No Hit
AGCCTAGGAAACGAAGCCTCAAAAACGCAGTTTCACGCAAATAATTAGGGGAAAGCTGAATTTTGGATATG10.8547008547008548No Hit
TTCCACCCCCCGCCTGC10.8547008547008548No Hit
TACTAACTGATAGTCTGCAAGATGCTGGAGCATACATGGAGATTATACCGATCTCAAGAGAGGAATGCAGGACAC10.8547008547008548No Hit
TCCACCCCCCTCCTGCCTATATGAGTTGAAC10.8547008547008548No Hit
TATTTCCACCCCCCGCCTGCCTATATGAGTT10.8547008547008548No Hit
TTCCACCCCCCGCCT10.8547008547008548No Hit
TTGGGAGCGCTAGTAGTAAAAACATCACTC10.8547008547008548No Hit
TATAATACATTCTTAAATAATACCCCCCAAG10.8547008547008548No Hit
CTGTATGTAGAAACTCCTAGTGTAGATTACAGGCCCTTGTTCTAGCCATTTGCTAGAAACCCT10.8547008547008548No Hit
TTATTACGGCCGCCGATTTGTTTCTCTT10.8547008547008548No Hit
TCCACCCCTCGCCTGCCTATATGAGTTGAAC10.8547008547008548No Hit
CACCCCCTGCCTGCCTATATGAGTTGAACC10.8547008547008548No Hit
TTTCCACCCCCCGCCTGCCTATATGAGTTG10.8547008547008548No Hit
CACCCCCCCGCCTGCCTATATGAGT10.8547008547008548No Hit
CACCCCCCGCCTG10.8547008547008548No Hit
TTCCACCCCCCTCCTGCCTATATGAGT10.8547008547008548No Hit
TACGGCCGCCGATTTGTTTCTCTTGGCGAG10.8547008547008548No Hit
TTATTACGGCCGCCGATTTGTTTCTCTTGG10.8547008547008548No Hit
TTCAATTTATTTATTACGGCCGCCGATTT10.8547008547008548No Hit
CCACCCCCCGCCTGTCTATATGAGTTGAACC10.8547008547008548No Hit
TTTCCACCTCCCGCCTGCCTATATGA10.8547008547008548No Hit
TGCCACTTGGGAGCGCTAGTAGTAAAAACAG10.8547008547008548No Hit
TCTGGACTCCCACCCTTCCAGAATGTGAAGGA10.8547008547008548No Hit
AACCCCGGCTTG10.8547008547008548No Hit
TTACGGCCGCCGATTTGTTTCTCTTGGC10.8547008547008548No Hit
TTCCACCCCCCGCCTGCCTCTATGAGTTG10.8547008547008548No Hit
ATTTCCACCCCCCGCCTGCCTATATG10.8547008547008548No Hit
TTTCCACCCCCCGCCTGCCT10.8547008547008548No Hit
TAATTAGGGGAAAGCTGAATTTTGGATATG10.8547008547008548No Hit
TTTTTCCACCCCCCGCCTGCCTATATGA10.8547008547008548No Hit
TGAAAGTTCAATTTATTTATTACGGCCGCCGATTTGTTTCTC10.8547008547008548No Hit
GCCTAGGAAACGAAGCCTCAAAAACGCCT10.8547008547008548No Hit
CCCCCCGCCTGCCTATATGAGTTGAACCC10.8547008547008548No Hit
TTTTCCAAAAA10.8547008547008548No Hit
GGAAAGCTGAATTTTGGATATGTGATAGAAC10.8547008547008548No Hit
TATGAGTTGAACCAATCTCATGTGGGCAG10.8547008547008548No Hit
CCGCCTGCCTATATGAGTTGAACC10.8547008547008548No Hit
CCACCCCCCTCCTGCCTATATGAGTTG10.8547008547008548No Hit
CCCCCCGCCTGCCTATATGAGTT10.8547008547008548No Hit
CCCCCCGCCTGCCTATATGAG10.8547008547008548No Hit
TCCCCCCGCCTG10.8547008547008548No Hit
TATTTGACTGCC10.8547008547008548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)