FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.16656.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.16656.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133
Sequences flagged as poor quality0
Sequence length12-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTCAGACTAGAGACAACCCGAGAGC62029.067041725269572No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAGC21610.126582278481013No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAG1386.469760900140647No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGA713.3286451007969995No Hit
TCAGACTAGAGACAACCCGAGAGCTCAAT612.8598218471636194No Hit
TCAGTTAGTACACCACACCGGGAGACGAC552.5785278949835915No Hit
TTCCTGTCAGACTAGAGACAACCCGAGAG522.437880918893577No Hit
TAGGTTCTTGAGAATATCGGCACGGAGC452.109704641350211No Hit
TTGGAGGAAAACTACGGCTCAACCCGGGC401.875293014533521No Hit
TCCTGTCAGACTAGAGACAACCCGAGAG391.8284106891701828No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGA291.3595874355368025No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGC261.2189404594467885No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATA251.1720581340834506No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATAT251.1720581340834506No Hit
TTTGGAGGAAAACTACGGCTCAACCCGGGC251.1720581340834506No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAG241.1251758087201125No Hit
TACGCCCTTCTCTATACTGCCGAAACTGGT130.6094702297233943No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGCT130.6094702297233943No Hit
TTTCCTGTCAGACTAGAGACAACCCGAGAGC120.5625879043600562No Hit
TCCAAGGACCCATCTCTCACGTGATTAGCA110.5157055789967182No Hit
TAATCACATCGTAATCTGTCTCTGACAGAATGTTTGACGCCT110.5157055789967182No Hit
TCCCCTGGCTGTACCAATTGCCTGACAAAT110.5157055789967182No Hit
CTTTTCCTGTCAGACTAGAGACAACCCGAG100.46882325363338023No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAA90.42194092827004215No Hit
TCTACCCCACCACAGAGCAAAGAATCCTCCC90.42194092827004215No Hit
TTCTTGAGAATATCGGCACGGAGCTCGC90.42194092827004215No Hit
TCTTCGTCGAGACGAGAATTCATTATACGC90.42194092827004215No Hit
TGTCAGACTAGAGACAACCCGAGAGCTCAAT80.3750586029067042No Hit
TAGGTTCTTGAGAATATCGGCACGGAGCT80.3750586029067042No Hit
TTAACGGTTTGGTCACCACACTTGAATGCC70.32817627754336615No Hit
TCAGACTAGAGACAACCCGAGAGCTCAA70.32817627754336615No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACT70.32817627754336615No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGCA60.2812939521800281No Hit
TGTAATCATAGAACCTGAACCACGGTAAACC60.2812939521800281No Hit
TGCATACGCCCTTCTCTATACTGCCGAAAC60.2812939521800281No Hit
TTCGTAATCCGCCTCACCTGTCCCTAACTG60.2812939521800281No Hit
TAATATAAAATGGAGGGTTAATTAGAGT50.23441162681669012No Hit
TTAGTACACCACACCGGGAGACGACATGTC50.23441162681669012No Hit
TTGAGAAAATCGGCACGGAGCTCGCATC50.23441162681669012No Hit
TACTGCATTGAAACCGGAGTCCGAAAAAT50.23441162681669012No Hit
CCTGTCAGACTAGAGACAACCCGAGAGC50.23441162681669012No Hit
TGTTTGACGCCTTCATTCCCTCTGGCAGC50.23441162681669012No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGCC50.23441162681669012No Hit
TGAGAAAATCGGCACGGAGCTCGCATCGTT50.23441162681669012No Hit
TGAAGGAGGTTAGAATCAAAGTGAGCCT50.23441162681669012No Hit
TGAAATCTACCCCACCACAGAGCAAAGAATC50.23441162681669012No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAAT50.23441162681669012No Hit
TTAGTACACCACACCGGGAGACGACATGT40.1875293014533521No Hit
CTGTCAGACTAGAGACAACCCGAGAGCTCAATATGTCTTCGTCGAGACGAGAATTCATTA40.1875293014533521No Hit
TTCTTGAGAAAATCGGCACGGAGCTCGC40.1875293014533521No Hit
TGCCCATGTTGCCTCCCGATTAGTACTC40.1875293014533521No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAGC40.1875293014533521No Hit
GACTATCAGTTAGTACACCACACCGGGA40.1875293014533521No Hit
TCGATACAATCTCTCAACCTGTTGCTTT40.1875293014533521No Hit
TTGGCCGCTAGAGTGCCACCACTGGTGCATAAA40.1875293014533521No Hit
ATGTCAATAGGTTCTTGAGAATATCGGCACGGAGCTCGCATCGTTTCTTTTCAAACA40.1875293014533521No Hit
TCTGTCTCTGACAGAATGTTTGACGCCT40.1875293014533521No Hit
TATGACAGACCAGAAACATTGCCCTAACTA40.1875293014533521No Hit
TAGGTTCTTGAGAAAATCGGCACGGAGCT40.1875293014533521No Hit
TATCAGTTAGTACACCACACCGGGAGAC40.1875293014533521No Hit
TTTCCTGTCAGACTAGAGACAACCCGAG40.1875293014533521No Hit
TCGTCACCTCCGAGATCTAGTAGGTCAGAT40.1875293014533521No Hit
TCACCTCCGAGATCTAGTAGGTCAGATTC40.1875293014533521No Hit
TCCTGTCAGACTAGAGACAACCCGAGAGCTCAAA40.1875293014533521No Hit
TATGACAGACCAGAAACATTGCCCAAACTA30.14064697609001406No Hit
TTGGAGGAAAACTACGGCTCAACCCGTGC30.14064697609001406No Hit
TTTTCCTGTCAGACTAGAGACAACCCGAGAG30.14064697609001406No Hit
TTGGTCAAGCAGGGCAGGATTTGATAGGA30.14064697609001406No Hit
TGTCAGACTAGAGACAACCCGAGAGCTC30.14064697609001406No Hit
TCAATAGGTTCTTGAGAATATCGGCACGG30.14064697609001406No Hit
TTCCTGTCAGACTAGAGACAACCCGAGA30.14064697609001406No Hit
TCCAGAGTTTGCCCATGTTGCCTCCCAATT30.14064697609001406No Hit
TATGGGTATTTGGTCAAGCAGGGCAGGA30.14064697609001406No Hit
TCTTGAGAATATCGGCACGGAGCTCGCATC30.14064697609001406No Hit
TTGGTCAAGCAGGGCAGGATTAGATAGG30.14064697609001406No Hit
TCAGACTAGAGACAACCCGAGAGCTCAATAA30.14064697609001406No Hit
TCATAGAACCTGAACCACGGTAAACCAATG30.14064697609001406No Hit
TTCTTGAGAATATCGGCACGGAGCTCGCA30.14064697609001406No Hit
TATGACAGACCAGAAACATTGCCCAAACTAT30.14064697609001406No Hit
TACGCAAAATCTTCTGGGCTAACTTTGGC30.14064697609001406No Hit
TGTGTACCACCTTCACAAGCTTGTCTCCTT30.14064697609001406No Hit
TAGGTTCTTGAGAATATCGGCACGGAG30.14064697609001406No Hit
TCAGATTCGTAATCCGCCTCACCTGTCCCT30.14064697609001406No Hit
TCATTCCCTCTGGCAGCGCACCTTTTCCTC30.14064697609001406No Hit
TCAGACTAGAGACAACCCGAGAGCTCA30.14064697609001406No Hit
TCGTAATCCGCCTCACCTGTCCCTAACTG30.14064697609001406No Hit
TTGAAATTGGAAATCGTAGGATTCATGAGTTATGTATGTGTACCACCTTCACAAGCTTGTCTCCTTCTGGGTATG30.14064697609001406No Hit
CTTTTCCTGTCAGACTAGAGACAACCCGAGAGCTCAA30.14064697609001406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)