FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.22483.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.22483.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length11-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTGCAAGCGCTCTGAAAAAGTTGCCGACCCCT412.5No Hit
TATGACTGCGCACGCGAGCTCAGTTTTTAAAGCTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCCC26.25No Hit
GTACTGGTCCG26.25No Hit
ATGACTGCGCACGCGAGCTCAGTTTTTAAAGCTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCCCTGGACTG26.25No Hit
TATGCCTGCCATGGCCGATCTTTTTCTTCT26.25No Hit
ACTGCGCACGCGAGCTCAGTTTTTAAAGCTGTTTTTGCACGCGCTCTGAA26.25No Hit
TTTGTTCAAAATATGACTGCGCACGCGAGC13.125No Hit
TTCCTAGGGATACAGCACTCGCACCTCCT13.125No Hit
TTTCCTAGGGATACAGCACTCGCACCTCCT13.125No Hit
CGGCGTCCCAATCGCCCCGTTCCACAG13.125No Hit
ATGACTGCGCACGCGAGCTCAGTTTTCAAAGCTGTTTTTGCACGCGCTCT13.125No Hit
TTTTAAAGCTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCCCTGCCATAGACA13.125No Hit
TGTTCAAAATATGACTGCGCACGCGAGCTC13.125No Hit
TTATAATGCGCACTTAGAGCCTTTTGCACAA13.125No Hit
TGCACGTGCTCTGAAAAAGTTGCCGACCCC13.125No Hit
GTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCCCTGA13.125No Hit
TTGTTCAAAATATGACTGCGCACGCGAG13.125No Hit
CAAAATATGACTGCGCACGCGAGCTCAGTTTTTAAAGCTGTTTTTGCA13.125No Hit
TTCAAAATATGACTGCGCCCGCGAGCTCAGTTTTCAAAGCTGTTTTCGCA13.125No Hit
CTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCC13.125No Hit
TATGCCTGCCATGGCCGATCTTTTTCTAATT13.125No Hit
TTTTGCACGCGCTCTGAAAAAGTTGCCGACCCCTGACT13.125No Hit
TATGCCTGCCATGGCCGATCTTTTTGTTCT13.125No Hit
TTTTAAAGCTGTTTTTGCACGCGCTCTGAAAAAGTTGCCGACCC13.125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)