FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.2812.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.2812.forward-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length12-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAGAACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGC68.21917808219178No Hit
CCTGTGAAGAAACTGTGCCCGGA45.47945205479452No Hit
AAAAGGACCAAGAAAAATCCATTACTTGCAAATCGGTAGGGAATC34.10958904109589No Hit
ACTGAAACACTGGCTACATTGCCTGAACCTGCATCCCAAAAACCAAGACA34.10958904109589No Hit
GGGAGAACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGC34.10958904109589No Hit
CCTGTGAAGAAACTGTGCCCGGAGAA34.10958904109589No Hit
AATTGTACGATTACTCGAAATGTGTAGGTCAACCGAAGGAAGTCT34.10958904109589No Hit
TCCCAGACCGACGGCCCCTGTGAAGAAACT34.10958904109589No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAAGC22.73972602739726No Hit
ATATCTACCAGCAAACCGCCAATTATGGATGTCGAGTCGGACGAAGAGTTATTCCCCGGCATGAGTTCA22.73972602739726No Hit
CCTGTGAAGAAACTGTGCCCGGAGA22.73972602739726No Hit
TTCCTATCAATCCAACCAGTGCAGATGTGCCCATATCTACCAGCAAACCGC22.73972602739726No Hit
CTGTGAAGAAACTGTGCCCGGAGAAA22.73972602739726No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAA22.73972602739726No Hit
CAGAACACCACGACCACGTCCACGTTATACACGACT22.73972602739726No Hit
GGGAGAACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGCTCATATTTCAAACGCCTCAC11.36986301369863No Hit
TTAGGCAGGCAAGTAGAATGGTATAAAGC11.36986301369863No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGAAAGC11.36986301369863No Hit
TGCCTGAACCTGCATCCCAAAAACCAAGAC11.36986301369863No Hit
CTGTGAAGAAACTGTGCCCGGAGA11.36986301369863No Hit
GCCCCTGTGAAGAAACTGTGCCCGGAG11.36986301369863No Hit
TTCTTAGGCAGGCAAGTAGAATGGTATAAAGCTTTACATTTCTCACAAGAAACGAGTTTGGTTTT11.36986301369863No Hit
CCTGTGAAGAAACTGTGCCCGGAGAAA11.36986301369863No Hit
TTTTAGAACTTCCTTCATCCATTGAAAGC11.36986301369863No Hit
CATCGCAGAACACCACGACCACGTCCACGTTATACACG11.36986301369863No Hit
ATTTTCATCATCATGTGGGGCTAAAATAGGCAGGCAAGTAGA11.36986301369863No Hit
CCCCTGTGAAGAAACTGTGCCCGGAGA11.36986301369863No Hit
GCCCCTGTGAAGAAACTGTGCCCGGAGAAAGCT11.36986301369863No Hit
TTCGGAACCACCCTGCTGCGCCGTTGAA11.36986301369863No Hit
GGTTCGTTCGGA11.36986301369863No Hit
AGGAGTACGAAAAGTTATTAATAACGCATTTACCGTCATCGCAGAACACC11.36986301369863No Hit
TTTTACATGAAATCTATCTCTTACCAAGTTCCAGACGATCTACAACAATATATACACGAGGAGTACGAAAAGTTA11.36986301369863No Hit
TAGCAACTGAAACACTGGCTACATTGCCTGAACCTGCATCCCAAAAACCAAGACATTCATCTGGTTGTGAATAGT11.36986301369863No Hit
TAGGCAGGCAAGTAGAATGGTATAAAGCT11.36986301369863No Hit
ATTTACCGTCATCGCAGAACACCACGACCACGTCCACGTTATACACGACTGTTCCT11.36986301369863No Hit
TCGCAGAACACCACGACCACGTCCACGTTATACACG11.36986301369863No Hit
TGAAGCTCGAATTGTACGATTACTCGAAATGTGTAGGTC11.36986301369863No Hit
TAGGCAGGCAAGTAGAATGGTATAAAGCA11.36986301369863No Hit
TTTACCGTCATCGCAGAACACCACGACCACGTCCACG11.36986301369863No Hit
CCCCTGTGAAGAAACTGTGCCCGGA11.36986301369863No Hit
TGTACGATTACTCGAAATGTGTAGGTCAACCGAAGGAA11.36986301369863No Hit
ACAATTACTCGAGTTCGTTCGGAACCACCCTGCTGCGCCGTTGAAGCT11.36986301369863No Hit
TTTACCGTCATCGCAGAACACCACGACCACGTCCACGTTATACACGACTG11.36986301369863No Hit
ATCACTCGTACTCCGGTTTTTGTCATTACAAATGAAGACATTTTTCCTCAAGACAATGCTTTTCGAACTC11.36986301369863No Hit
GGATGTCGAGTCGGACGAAGAGTTATTCCCCGGCATGAGTTCACAATTAAGTATGCCGTCAATTCCGTGCTCTAT11.36986301369863No Hit
ACTGGAAGCAAGCAACTTGGAAGACATTGTGCACGGTTTCATCCAGTGCGCAGACGGGGCAACTGTTGAGACA11.36986301369863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)