FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.2812.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.2812.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053
Sequences flagged as poor quality0
Sequence length19-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTGAAGTCCCGAGTTATCGCTTTCTG1185.747686312713103No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGT1125.455431076473453No Hit
TACTTAATTGTGAACTCATGCCGGGGAA894.335119337554798No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACATC602.922552362396493No Hit
TCCCGAGTTATCGCTTTCTGTAATTGTCTC542.6302971261568433No Hit
TCCATAATTGGCGGTTTGCTGGTAGATA502.4354603019970775No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTG462.2406234778373113No Hit
TAACTCTTCGTCCGACTCGACATCCATA381.8509498295177789No Hit
TACTTAATTGTGAACTCATGCCGGGGAAT311.509985387238188No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGTC291.412566975158305No Hit
TGCTTCCAGTAGACTTCCTTCGGTTGACCT271.3151485630784217No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAG251.2177301509985388No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACT251.2177301509985388No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACA241.169020944958597No Hit
TCACAGGGGACGTCGGTCTGGGACACTC221.071602532878714No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGC221.071602532878714No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTC200.974184120798831No Hit
TCACAGGGGCCGTCGGTCTGGGACACTC190.9254749147588894No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCGT180.876765708718948No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTGA170.8280565026790063No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGAGT160.7793472966390648No Hit
TCTTGAAGTCCCGAGTTATCGCTTTCTGT150.7306380905991232No Hit
TTCACAGGGGACGTCGGTCTGGGACACTC140.6819288845591817No Hit
TTCAACGGCGCAGCAGGGTGGTTCCGAAC140.6819288845591817No Hit
TCGTGTATAACGTGGACGTGGTCGTGGT140.6819288845591817No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGTT130.6332196785192401No Hit
TAGATATGGGCACATCTGCACTGGTTGGAT120.5845104724792985No Hit
TACTTAATTGTGAATTCATGCCGGGGAA120.5845104724792985No Hit
TCCTCCCATACTTGCGCTTGCTGATCGGG120.5845104724792985No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATT110.535801266439357No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCAC110.535801266439357No Hit
TCCGGGCACAGTTTCTTCACAGGGGCCGTC110.535801266439357No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCAC110.535801266439357No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACTC110.535801266439357No Hit
TCCGAACGAACTCGAGTAATTGTTCTCCCG100.4870920603994155No Hit
TATTGTTGTAGATCGTCTGGAACTTGGT100.4870920603994155No Hit
TCGGTCTGGGACACTCTTGGAAGGTCAGC100.4870920603994155No Hit
TTCGAGCTTCATTACCAAATCGTGGGATC100.4870920603994155No Hit
TGCTGATCGGGAGTCAAATGCACTTGCACTT90.438382854359474No Hit
TGGGCACATCTGCACTGGTTGGATTGATA90.438382854359474No Hit
TGCTTGCTTCCAGTAGACTTCCTTCGGT90.438382854359474No Hit
TACTTAATTGTGAATTCATGCCGGGGA90.438382854359474No Hit
TCTCCATAATCCATTAGTGACCAAGCCGCC80.3896736483195324No Hit
TGCCCCGTCTGCGCACTGGATGAAACCG80.3896736483195324No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTG80.3896736483195324No Hit
TTAGTGACCAAGCCGCCAATTCAATTGCT70.34096444227959083No Hit
TTTGAAGAATTCTAGCACCACGATGAAAT70.34096444227959083No Hit
TTGCCCCGTCTGCGCACTGGATGAAACCG70.34096444227959083No Hit
TGAAACCGTGCACAATGTCTTCCAAGTTGC70.34096444227959083No Hit
TTGGCGGTTTGCTGGTAGATATGGGCACAT70.34096444227959083No Hit
TCCAAGTTGCTTGCTTCCAGTAGACTTCCT70.34096444227959083No Hit
TCGACATCCATAATTGGCGGTTTGCTGGT70.34096444227959083No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGTC70.34096444227959083No Hit
TCATCTCCAGATTCGCATGCTGACGCGAC60.2922552362396493No Hit
TAATTGGCGGTTTGCTGGTAGATATGGGCACA60.2922552362396493No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGGG60.2922552362396493No Hit
TGAAATAGTGGGGGCAGCAGACCTATAGTCACGCCAGT60.2922552362396493No Hit
TTGTCTCAACAGTTGCCCCGTCTGCGCACT60.2922552362396493No Hit
TGACGGTAAATGCGTTATTAATAACTTTTCGTACTCCTCGTGTATATATT60.2922552362396493No Hit
TAGATATGGGCACATCTGCACTGGTTGGA60.2922552362396493No Hit
TCTGTAATTGTCTCAACAGTTGCCCCGTC60.2922552362396493No Hit
TGCTGGTAGATATGGGCACATCTGCACTG50.24354603019970775No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCT50.24354603019970775No Hit
TTCGAGCTTCATTACCAAATCGTGGGAT50.24354603019970775No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACT50.24354603019970775No Hit
TCCATAATCCATTAGTGACCAAGCCGCCAAT50.24354603019970775No Hit
TACAGGGTCAATTGTTGCGTAGTAATCTCC50.24354603019970775No Hit
TAGTCGGCTTCTTTCATAATTATTTGATGC40.1948368241597662No Hit
TTTGCTGGTAGATATGGGCACATCTGCACT40.1948368241597662No Hit
TCTGCACTGGTTGGATTGATAGGAACAGTC40.1948368241597662No Hit
TCTTCGTCCGACTCGACATCCATAATTGGC40.1948368241597662No Hit
TCTTCACAGGGGCCGTCGGTCTGGGACACT40.1948368241597662No Hit
TACTTGCGCTTGCTGATCGGGAGTCAAA40.1948368241597662No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAAT40.1948368241597662No Hit
TACTTAATTGTGAACTCATGCCGGGGAATAACTCTTCGTCCGACTCGACA40.1948368241597662No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGGG40.1948368241597662No Hit
TGTAGATCGTCTGGAACTTGGTAAGAGA40.1948368241597662No Hit
TCGATGAAATAGTGGGGGCAGCAGACCT40.1948368241597662No Hit
TATGAGCTTCAACGGCGCAGCAGGGTGG40.1948368241597662No Hit
TGCACTTTGGCTCCGGATGCTTTCTC40.1948368241597662No Hit
TTCCTCCCATACTTGCGCTTGCTGATCGG40.1948368241597662No Hit
TATGGGCACATCTGCACTGGTTGGATT40.1948368241597662No Hit
TGTCTCAACAGTTGCCCCGTCTGCGCACTG40.1948368241597662No Hit
TGAGTACAGTTTGAAGAATTCTAGCACC40.1948368241597662No Hit
TTGGTCCAGTGAGGCGTTTGAAATATGAGC40.1948368241597662No Hit
TCACAGGGGCCGTCGGTCTGGGACACTCT40.1948368241597662No Hit
TCCGGGCACAGTTTCTTCACAGGGGACG40.1948368241597662No Hit
TGACCAAGCCGCCAATTCAATTGCTCTGTT40.1948368241597662No Hit
TTCGTCCGACTCGACATCCATAATTGGCG40.1948368241597662No Hit
GAGTAATTGTTCTCCCGTATTTCTGGGGC40.1948368241597662No Hit
TATGAGCTTCAACGGCGCAGCAGGGTG40.1948368241597662No Hit
CATACTTGCGCTTGCTGATCGGGAGTCAAATGCACTTGCACTTTGGCTCCGGATGCTTTCTCCGGGC40.1948368241597662No Hit
TGTTGTAGATCGTCTGGAACTTGGTAAGA40.1948368241597662No Hit
TTTGCTGGTAGATATGGGCACATCTGCACTG40.1948368241597662No Hit
TTGAAATATGAGCTTCAACGGCGCAGCAGGG30.14612761811982464No Hit
TGCCCCGTCTGCGCACTGGATGAAACCGTA30.14612761811982464No Hit
TCCGGGCACAGTTTCTTCACAGGGGACGT30.14612761811982464No Hit
TGATATTGACCCAACTAGACTAGTAGAACT30.14612761811982464No Hit
TAGATATGGGCACATCTGCACTGGTTGG30.14612761811982464No Hit
TTCTGTAATTGTCTCAACAGTTGCCCCGT30.14612761811982464No Hit
TGCTTCCAGTAGACTTCCTTCGGTTG30.14612761811982464No Hit
TTCACAGGGGCCGTCGGTCTGGGACACTCC30.14612761811982464No Hit
TTCGGCCCATAGCATTGACATTTTCTTCATC30.14612761811982464No Hit
TCTGTTGTTGAGTACAGTTTGAAGAATTCTAGCACCACAATGAAATAAAGCAAACTTCGATTTGGTCCA30.14612761811982464No Hit
TCACAGGGGACGTCGGTCTGGGACACTCT30.14612761811982464No Hit
TAGAGATGGGCACATCTGCACTGGTTGGAT30.14612761811982464No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTCT30.14612761811982464No Hit
TAGATATGGGCACATCTGCACTGGTTG30.14612761811982464No Hit
TAGGAGGTGAACTCTCAAGGGATTCGTATGCATTCAAATCCAAATGAAATAGATTGTTTCGAACCAATCTACATA30.14612761811982464No Hit
TGAGTACAGTTTGAAGAATTCTAGCACCACAATGAA30.14612761811982464No Hit
TTCTTCACAGGGGCCGTCGGTCTGGGAC30.14612761811982464No Hit
TGCTCGATGAAATAGTGGGGGCAGCAGAC30.14612761811982464No Hit
AGTTTCTTCACAGGGGCCGTCGGTCTGG30.14612761811982464No Hit
TCGGTCTGGGACACTCTTGGAAGGTCA30.14612761811982464No Hit
TGATAACTTGATCCTCTTGAAATTTAACTCTAATGGAAC30.14612761811982464No Hit
TGCACTGGTTGGATTGATAGGAACAGTC30.14612761811982464No Hit
TTATCGCTTTCTGTAATTGTCTCAACAGTTGCCCCGTCTGCGCACTGGATGAAACCGTGCACAATGTCTTCCAAG30.14612761811982464No Hit
TCCAGATTCGCATGCTGACGCGACCACGCT30.14612761811982464No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCT30.14612761811982464No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCGA30.14612761811982464No Hit
ACAGTTTGAAGAATTCTAGCACCACAATG30.14612761811982464No Hit
TGTTCTCCCGTATTTCTGGGGCGCTTAAACTGCCCATCTCGCCT30.14612761811982464No Hit
TCATCTCCAGATTCGCATGCTGACGCGACC30.14612761811982464No Hit
TTCTGAAATTGTCTCAACAGTTGCCCCGTC30.14612761811982464No Hit
TTCATCTCCAGATTCGCATGCTGACGCGACC30.14612761811982464No Hit
TGCCCATCTCGCCTTTCTTGAAGTCCCG30.14612761811982464No Hit
TGAAATATGAGTTTCAACGGCGCAGCAGG30.14612761811982464No Hit
CCCCGTCTGCGCACTGGATGAAACCGTGC30.14612761811982464No Hit
TACAGGGTCAATTGTTGCATAGTAATCTCC30.14612761811982464No Hit
TGTATAACGTGGACGTGGTCGTGGTGTT30.14612761811982464No Hit
TCATCTCCAGATTCGCATGCTGACGCGACCACGCTGCTGCTCGA30.14612761811982464No Hit
TTAGATTGATAATTCCCTGAGGATGTTCCTCCC30.14612761811982464No Hit
TATGGGCACATCTGCACTGGTTGGA30.14612761811982464No Hit
TACAATTCGAGCTTCATTACCAAATCGTGGGATCGAT30.14612761811982464No Hit
TGCTCTGTTGTTGAGTACAGTTTGAAGAATTCTAGCACCACGATGAAATA30.14612761811982464No Hit
TGTAAAACGTTTGCTTTGACAGGTATCCT30.14612761811982464No Hit
TCTGGGACACTCTTGGAAGGTCAGCTTCCTC30.14612761811982464No Hit
TACTTAATTGTGAACTCATGCCGGGGAATA30.14612761811982464No Hit
TTCGCATGCTGACGCGACCACGCTGCTGC30.14612761811982464No Hit
TGAAGTCCCGAGTTATCGCTTTCTGTAATTGTCTCAACAGTTGCCCCGTC30.14612761811982464No Hit
TTCCAGTAGACTTCCTTCGGTTGACCTACA30.14612761811982464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)