FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.28821.forward-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.28821.forward-mapped.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences15
Sequences flagged as poor quality0
Sequence length11-34
%GC7

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAAAAAAAAAAAAA533.33333333333333No Hit
AAAAAAAAAAAAAAA533.33333333333333No Hit
AAAAAAAAAAAAAAAAAAAAAAA213.333333333333334No Hit
GAAATGGGAGA16.666666666666667No Hit
AAAAAAAAAAAAAAAAAAAAAA16.666666666666667No Hit
TGCGACATGGACAGTAGATCAGAAGAAGATCACC16.666666666666667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)