FastQCFastQC Report
Mon 30 Nov 2020
S33.raw.28821.reverse-mapped.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameS33.raw.28821.reverse-mapped.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160
Sequences flagged as poor quality0
Sequence length12-75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAAGTACCCCTGAACCTTCAGGCCCA2012.5No Hit
TAATCGCTGTACATCTCTCTGAACCGTGG74.375No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATAGG74.375No Hit
TACGTCTGTATGACTCAGCCCCGTACATA63.75No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCCT53.125No Hit
TCGGAGCCATCAACATGCAAAGTTAAGCCT53.125No Hit
TGAAGAACTCTCGTGCTTGCGCTGGGGT42.5No Hit
TGATCTTCCAACTTGACCTCCTCTGGTACT42.5No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCAAT31.875No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCC31.875No Hit
TCCAATAGAACTCGTGAATCGCAGTGGGC31.875No Hit
TAGAACTCGTGAATCGCAGTGGGCTGC31.875No Hit
TGCAATGGCCGCTTGGCATAGATGGGG21.25No Hit
TGAAGAACTCTCGTGCTTGCGCTGGG21.25No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGT21.25No Hit
TCTCCCATTTCTTTACCAAGTACCCCTGAAC21.25No Hit
TCGCTGTACATCTCTCTGAACCGTGGGA21.25No Hit
TCAACGATCTTATACAGAGGCCACGCGGC21.25No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGG21.25No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCCGGC21.25No Hit
TACTAGTCTCTTCGCAGGAGCTGGACCGCCT21.25No Hit
TTCACTAAGAGTTTGTATCCAGATGTCGAG21.25No Hit
GTGTCGGAGCCA21.25No Hit
TCCATGTCGCAAACCCTCTTTCTCCTCCA21.25No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCA21.25No Hit
TCCGCTGCGCTAGGTACTAGTCTCTTCGC21.25No Hit
TAACCTACCATTCTGAGACAGTTGATCTTCC21.25No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCG21.25No Hit
TCCTAGGACCTCTTTAGCTTGACTTATAACAACCGATGTATCTAATTTCTTAGCTACCA21.25No Hit
TCTTCCAACTTGACCTCCTCTGGTACTTG21.25No Hit
TAAGACTTTCACGATATCAACTTGTCCCT21.25No Hit
TTTCAAGGGAGATGGGCTATTCACTAGG10.625No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGTT10.625No Hit
TCCAACCTCCTGAGAACATCTACGCATCTT10.625No Hit
TTACCAAGTACCCCTGAACCTTCAGGCCCA10.625No Hit
CTAGCAGCTTCCCGGCCTCATTTATTCCTAGGACC10.625No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATA10.625No Hit
TACGTCTGTATGACTCAGCCCCGTACATACAAGTCCATGCAATGGCCGCTTGGCATAGATGGGGCCACT10.625No Hit
TTGAAGAACTCTCGTGCTTGCGCTGG10.625No Hit
TAGAACTCGTGAATCGCAGTGGGCTGCGGTTTTTGGT10.625No Hit
TAGTGAAATCCGATACCCAGGTGATCTTC10.625No Hit
TCAACATGCAAAGTTAAGCCTGGCTCAAGCT10.625No Hit
TACCAAGTACCCCTGAACCTTCAGGCCCAATGTCTGGGTGAACACCCATCTCCTCTAGCAGCTTCCCGGCCTCAT10.625No Hit
TTTACCAAGTACCCCTGAACCTTCAGGCCC10.625No Hit
TCTGAACCGTGGGAGATGAGTAAATGGCCTTA10.625No Hit
TTTAACTTGCCCTGCTTCTCTCACAGACA10.625No Hit
TTTCTTTACCAAGTACCCCTGAACCTTCAGGCC10.625No Hit
TCACGATATCAACTTGTCCCTTTGACACCG10.625No Hit
TAACCTACCATTCTGAGACAGTTGATCTTC10.625No Hit
TCTACATCGATAACTACCCCGTCATCTGG10.625No Hit
TACAACCATCCCACCACCATCTACTCCAACCT10.625No Hit
GAAGAACTCTCGTGCTTGCGCTGGGGT10.625No Hit
AACATCGACATGT10.625No Hit
TGCGATATGGTTTTTCAAGGGAGATGGGC10.625No Hit
TGAACATCGACATGT10.625No Hit
ATAGAACTCGTGAATCGCAGTGGGCTGC10.625No Hit
ACCCATCTCCTCTAGCAGCTTCCCGG10.625No Hit
GAGGACCAAAGGCTGTCTCATCTGCGATAGGGTA10.625No Hit
TAGGTACTAGTCTCTTCGCAGGAGCTGGAC10.625No Hit
TACATCGATAACTACCCCGTCATCTGG10.625No Hit
TAGGACCTCTTTAGCTTGACTTATAACAA10.625No Hit
TTTACCAAGTACCCCTGAACCTTCAGGCCCA10.625No Hit
TTTGAAGAACTCTCGTGCTTGCGCTGGG10.625No Hit
TCATACGTCTGTATGACTCAGCCCCGTA10.625No Hit
TTGTATCCAGATGTCGAGATGAACAACTC10.625No Hit
CGATGTTGGGTTT10.625No Hit
TAGTCTCTTCGCAGGAGCTGGACCGCCTCAA10.625No Hit
TATGACTCAGCCCCGTACATACAAGTCCAT10.625No Hit
TACGTCTGTATGACTCAGCCCCGTACAT10.625No Hit
TCTGCGATATGGTTTTTCAAGGGAGATGGGC10.625No Hit
TCTTTGAAGAACTCTCGTGCTTGCGCTG10.625No Hit
TATGGTTTTTCAAGGGAGATGGGCTATT10.625No Hit
TTCCAATAGAACTCGTGAATCGCAGTGGGC10.625No Hit
TCTAGCAGCTTCCCGGCCTCATTTATTCCTAGGACCTCTTT10.625No Hit
TCTGTCCATGTCGCAAACCCTCTTTCTCCT10.625No Hit
TTGATCTTCCAACTTGACCTCCTCTGGTACT10.625No Hit
TTTCTTTACCAAGTACCCCTGAACCTTC10.625No Hit
TCCTAGGACCTCTTTAGCTTGACTTATAACAACCGATGTATCTAATTTCTTAGCTACCAAATGGA10.625No Hit
AGGGGCAGAACTTTTTTCATTAGAGGACC10.625No Hit
TAGGGGCAGAACTTTTTTCATTAGAGGACC10.625No Hit
TGAACACCCATCTCCTCTAGCAGCTTCCC10.625No Hit
TTGAAGAACTCTCGTGCTTGCGCTGGGGT10.625No Hit
TCTTTACCAAGTACCCCTGAACCTTCAGG10.625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)